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Product Info Summary
| SKU: | M05879 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, ICC, WB |
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Product info
Product Name
Anti-Hu CD164 Purified Monoclonal Antibody
SKU/Catalog Number
M05879
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD164 Purified Monoclonal Antibody (Catalog# M05879). Tested in Flow Cytometry, IP, WB, ICC application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD164 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05879)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
67D2
Isotype
Mouse IgG1
Immunogen
Breast tumor cell line T-47D. The mouse monoclonal antibody 67D2 recognizes an extracellular class III epitope (not sensitive to sialidase, N-glycanase, O-glycosidase, and O-sialoglycoprotease) of CD164, a sialomucin expressed in hematopoietic myeloid and erythroid progenitors, activated basophils, and in various carcinomas and leukemic cells.
Cross-reactivity
Cross-reactivity with CLIC5b was not determined.
Reactive Species
M05879 is reactive to CD164 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
20.9 kDa
Background of CD164
CD164, also known as endolyn, is a type I transmembrane protein with heavily glycosylated extracellular part containing sialic acid and glycosaminoglycan residues. CD164 plays both adhesive and antiadhesive role and serves as a potent negative regulator for CD34+ CD38- hematopoietic progenitor cell proliferation. It has also been reported to be involved in myogenic differentiation and cancer metastasis. The adhesive and negative regulatory functions seem to depend on different posttranslational modifications of CD164 protein.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05879 is guaranteed for Flow Cytometry, IP, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 μg/ml.
Validation Images & Assay Conditions
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Flow cytometry analysis (surface staining) of human peripheral blood cells using anti-CD164 (67D2) purified, GAM-FITC.
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Western blotting analysis of human CD164 expression in various cell lines under reducing and non-reducing conditions using mouse monoclonal antibody 67D2. The same amount of total protein (20 µg) was loaded in each lane. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody.
Specific Publications For Anti-Hu CD164 Purified Monoclonal Antibody (M05879)
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