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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).|
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Human CD164 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human CD164 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CD164 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CD164 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1811)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: D24-D162
93.8 pg/ml - 6,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1811 is reactive to CD164 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).
EK1811 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CD164
Sialomucin core protein 24 also known as endolyn or CD164， is a protein that in humans is encoded by the CD164 gene. CD164 functions as a cell adhesion molecule. This gene is mapped to 6q21. The encoded protein also interacts with the C-X-C chemokine receptor type 4 and may regulate muscle development. Elevated expression of this gene has been observed in human patients with Sezary syndrome, a type of blood cancer, and a mutation in this gene may be associated with impaired hearing.
|EK1811-CAP||Anti-Human CD164 Pre-coated 96-well strip microplate||1|
|EK1811-ST||Human CD164 Standard||2 vials, 10 ng/tube|
|EK1811-DA||Human CD164 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human CD164 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CD164, Dilution ratio of 1:1, concentration in serum is 1-2ng/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CD164 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of CD164 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For CD164 (Source: Uniprot.Org, NCBI)
Sialomucin core protein 24
CD164 antigen; CD164 molecule, sialomucin; CD164; Endolyn; MGC-24; MGC-24v; MUC-24; Multi-glycosylated core protein 24; sialomucin CD164 DFNA66, MGC-24, MGC-24v, MUC-24, endolyn CD164 molecule sialomucin core protein 24|CD164 antigen, sialomucin|multi-glycosylated core protein 24*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CD164, check out the CD164 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CD164: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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