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Product Info Summary
| SKU: | M12477-10 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Histone H3 HIST1H3A Rabbit Monoclonal Antibody
SKU/Catalog Number
M12477-10
BM4715 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Histone H3 HIST1H3A Rabbit Monoclonal Antibody catalog # M12477-10. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Histone H3 HIST1H3A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M12477-10)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
HDE-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Histone H3
Reactive Species
M12477-10 is reactive to HIST1H3A in Human, Mouse, Rat
Observed Molecular Weight
17 kDa
Calculated molecular weight
15.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M12477-10 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Recommend Dilution
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
Tested application
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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Western blot analysis of Histone H3 expression in (1) HeLa cell lysate; (2) 3T3 cell lysate.
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Immunohistochemical analysis of paraffin-embedded Human tonsil, using the Antibody at 1:2000 dilution.
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Immunohistochemical analysis of paraffin-embedded Human colon cancer, using the Antibody at 1:6000 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat thymus, using the Antibody at 1:3000 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse spleen, using the Antibody at 1:3000 dilution.
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Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma, using the Antibody at 1:2000 dilution.
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Immunohistochemical analysis of paraffin-embedded mouse brain, using Histone H3 Antibody.
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Immunofluorescent analysis of Hela cells, using Histone H3 Antibody.
Specific Publications For Anti-Histone H3 HIST1H3A Rabbit Monoclonal Antibody (M12477-10)
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