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Product Info Summary
| SKU: | M01926 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Radixin Rabbit Monoclonal Antibody
SKU/Catalog Number
M01926
BM4489 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Radixin Rabbit Monoclonal Antibody catalog # M01926. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Radixin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01926)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
FBC-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Radixin
Reactive Species
M01926 is reactive to RDX in Human, Mouse, Rat
Observed Molecular Weight
80 kDa
Calculated molecular weight
68.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01926 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Recommend Dilution
WB 1:1000-5000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20
Tested application
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Validation Images & Assay Conditions
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Western blot analysis of Radixin using anti-Radixin antibody (M01926).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HUVEC whole cell lysates,
Lane 3: human U251 whole cell lysates,
Lane 4: human SiHa whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Radixin antigen affinity purified monoclonal antibody (Catalog # M01926) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Radixin at approximately 80 kDa. The expected band size for Radixin is at 80 kDa.
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IHC analysis of smc4 using anti-smc4 antibody (M01926).
smc4 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-smc4 Antibody (M01926) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Radixin Rabbit Monoclonal Antibody (M01926)
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6 Customer Q&As for Anti-Radixin Rabbit Monoclonal Antibody
Question
Is there a BSA free version of anti-Radixin Rabbit Monoclonal antibody M01926 available?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Radixin Rabbit Monoclonal antibody M01926 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-23
Question
See below the WB image, lot number and protocol we used for metanephros using anti-Radixin Rabbit Monoclonal antibody M01926. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-06
Question
I see that the anti-Radixin Rabbit Monoclonal antibody M01926 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-11-22
Question
Will M01926 anti-Radixin Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
H. Anderson
Verified customer
Asked: 2019-03-08
Answer
You can see on the product datasheet, M01926 anti-Radixin Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-03-08
Question
Is a blocking peptide available for product anti-Radixin Rabbit Monoclonal antibody (M01926)?
Verified Customer
Verified customer
Asked: 2017-07-03
Answer
We do provide the blocking peptide for product anti-Radixin Rabbit Monoclonal antibody (M01926). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-07-03
Question
Does anti-Radixin Rabbit Monoclonal antibody M01926 work on monkey IF with hippocampus?
Verified Customer
Verified customer
Asked: 2017-05-12
Answer
Our lab technicians have not validated anti-Radixin Rabbit Monoclonal antibody M01926 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Radixin Rabbit Monoclonal antibody M01926 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey hippocampus in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-05-12
