Anti-SREBF1 Antibody

Product Name

Anti-SREBF1 Antibody

View all SREBF1 Antibodies

SKU/Catalog Number

A00282

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-SREBF1 Antibody (Catalog # A00282). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

SREBF1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-SREBF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00282)

Host

Rabbit

Contents

SREBF1 antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

SREBF1 antibody was raised against a 17 amino acid peptide near the center of human SREBF1. The immunogen is located within amino acids 450 - 500 of SREBF1.

Cross-reactivity

SREBF1 antibody is human and mouse reactive. At least three isoforms of SREBF1 are known to exist. SREBF1 antibody is predicted not to cross-react with SREBF2.

Reactive Species

A00282 is reactive to SREBF1 in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

121.7 kDa

Background of SREBF1

The sterol regulatory element binding transcription factor 1 (SREBF1) is a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis (1). The related protein SREBF2 also binds SRE1 and activates transcription in an additive fashion to SREBF1 (2). SREBF1 is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1 (3). The SREBF1 proteins are important in the regulation of genes involved in lipid metabolism, while SREBF2 has been more closely associated with cholesterol synthesis and accumulation (4).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00282 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

SREBF1 antibody can be used for detection of SREBF1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information SREBF1

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