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Product Info Summary
| SKU: | M01871 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-TMEM173 Monoclonal Antibody
SKU/Catalog Number
M01871
BM5485 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-TMEM173 Monoclonal Antibody catalog # M01871. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TMEM173 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01871)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AEAO-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human TMEM173 Facilitator of innate immune signaling that promotes the production of type I interferon (IFN-alpha and IFN-beta) . Innate immune response is triggered in response to non-CpG double-stranded DNA from viruses and bacteria delivered to the cytoplasm.
Reactive Species
M01871 is reactive to TMEM173 in Human
Observed Molecular Weight
37 kDa
Calculated molecular weight
42.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01871 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Recommend Dilution
WB 1:500-2000
ICC/IF 1:50-200
FC 1:20
Validation Images & Assay Conditions
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Western blot analysis of TMEM173 using anti-TMEM173 antibody (M01871).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HEL whole cell lysates,
Lane 3: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM173 antigen affinity purified monoclonal antibody (Catalog # M01871) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM173 at approximately 37 kDa. The expected band size for TMEM173 is at 37,42 kDa.
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All lanes use the Antibody at 1:4K dilution for 1 hour at room temperature.
Specific Publications For Anti-TMEM173 Monoclonal Antibody (M01871)
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4 Customer Q&As for Anti-TMEM173 Monoclonal Antibody
Question
My question regarding product M01871, anti-TMEM173 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01871 anti-TMEM173 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-07
Question
What sample was tested in WB and IHC applications with M01871?
Verified customer
Asked: 2020-01-09
Answer
Western blot analysis of TMEM173 expression in HeLa cell lysate.
Boster Scientific Support
Answered: 2020-01-09
Question
Is there a BSA free version of anti-TMEM173 Monoclonal antibody M01871 available?
R. Mangal
Verified customer
Asked: 2018-11-06
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TMEM173 Monoclonal antibody M01871 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-11-06
Question
Is a blocking peptide available for product anti-TMEM173 Monoclonal antibody (M01871)?
T. Li
Verified customer
Asked: 2015-04-14
Answer
We do provide the blocking peptide for product anti-TMEM173 Monoclonal antibody (M01871). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2015-04-14
