Click image to see more details
Product Info Summary
| SKU: | A08583 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-TyrRS YARS Antibody
SKU/Catalog Number
A08583
Size
100μl
Form
Liquid
Description
Boster Bio Anti-TyrRS YARS Antibody catalog # A08583. Tested in WB, IHC, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TyrRS YARS Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08583)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the C-terminal region of human YARS. AA range:451-500
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A08583 is reactive to YARS in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
59.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08583 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
ELISA 1:10000-20000
Validation Images & Assay Conditions
Click image to see more details
Western Blot analysis of Hela cells using TyrRS Polyclonal Antibody diluted at 1:500. Secondary antibody was diluted at 1:20000
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human-colon-cancer, antibody was diluted at 1:200
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200
Specific Publications For Anti-TyrRS YARS Antibody (A08583)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-TyrRS YARS Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-TyrRS YARS Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Anti-TyrRS YARS Antibody
Question
I was wanting to use your anti-TyrRS antibody for WB for mouse brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse brain identification?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
You can see on the product datasheet, A08583 anti-TyrRS antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-27
Question
My question regarding product A08583, anti-TyrRS antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A08583 anti-TyrRS antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-21
Question
Will A08583 anti-TyrRS antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-01-14
Answer
It shows on the product datasheet, A08583 anti-TyrRS antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-01-14
Question
We are currently using anti-TyrRS antibody A08583 for rat tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2018-11-26
Answer
The anti-TyrRS antibody (A08583) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-11-26
Question
We need to test anti-TyrRS antibody A08583 on mouse brain for research purposes, then I may be interested in using anti-TyrRS antibody A08583 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
K. Jones
Verified customer
Asked: 2018-05-30
Answer
The products we sell, including anti-TyrRS antibody A08583, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-05-30
Question
Is a blocking peptide available for product anti-TyrRS antibody (A08583)?
Verified Customer
Verified customer
Asked: 2018-01-29
Answer
We do provide the blocking peptide for product anti-TyrRS antibody (A08583). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-01-29
Question
Does anti-TyrRS antibody A08583 work on zebrafish WB with brain?
N. Johnson
Verified customer
Asked: 2014-07-17
Answer
Our lab technicians have not validated anti-TyrRS antibody A08583 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-TyrRS antibody A08583 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-07-17
