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Product Info Summary
| SKU: | M00293 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Ret Rabbit Monoclonal Antibody
SKU/Catalog Number
M00293
BM4563 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Ret Rabbit Monoclonal Antibody catalog # M00293. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ret Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00293)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
FIF-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Ret
Reactive Species
M00293 is reactive to RET in Human, Mouse, Rat
Observed Molecular Weight
150, 175 kDa
Calculated molecular weight
124.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00293 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: SH-SY5Y cell
Validation Images & Assay Conditions
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Western blot analysis of Ret expression in SH-SY5Y cell lysate.
Specific Publications For Anti-Ret Rabbit Monoclonal Antibody (M00293)
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Customer Q&As
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4 Customer Q&As for Anti-Ret Rabbit Monoclonal Antibody
Question
We have been able to see staining in rat prostate. Are there any suggestions? Is anti-Ret Rabbit Monoclonal antibody supposed to stain prostate positively?
Verified Customer
Verified customer
Asked: 2018-04-10
Answer
From literature prostate does express RET. From Uniprot.org, RET is expressed in dorsal root ganglion, prostate, brain, fibroblast, thyroid papillary carcinoma, blood, leukocyte, peripheral blood leukocyte, among other tissues. Regarding which tissues have RET expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 7633441
Brain, Pubmed ID: 15489334
Fibroblast, Pubmed ID: 2734021
Leukocyte, Pubmed ID: 7581377
Peripheral blood leukocyte, Pubmed ID: 9360560
Prostate, Pubmed ID: 14702039
Thyroid papillary carcinoma, Pubmed ID: 2406025
Boster Scientific Support
Answered: 2018-04-10
Question
We are currently using anti-Ret Rabbit Monoclonal antibody M00293 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
J. Anderson
Verified customer
Asked: 2016-04-11
Answer
The anti-Ret Rabbit Monoclonal antibody (M00293) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-04-11
Question
My team were satisfied with the WB result of your anti-Ret Rabbit Monoclonal antibody. However we have observed positive staining in blood cell membrane using this antibody. Is that expected? Could you tell me where is RET supposed to be expressed?
E. Kulkarni
Verified customer
Asked: 2016-02-25
Answer
From literature, blood does express RET. Generally RET expresses in cell membrane. Regarding which tissues have RET expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 7633441
Brain, Pubmed ID: 15489334
Fibroblast, Pubmed ID: 2734021
Leukocyte, Pubmed ID: 7581377
Peripheral blood leukocyte, Pubmed ID: 9360560
Prostate, Pubmed ID: 14702039
Thyroid papillary carcinoma, Pubmed ID: 2406025
Boster Scientific Support
Answered: 2016-02-25
Question
We ordered your anti-Ret Rabbit Monoclonal antibody for WB on leukocyte last year. I am using rat, and We are going to use the antibody for IP next. I am interested in examining leukocyte as well as blood in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?
D. Mitchell
Verified customer
Asked: 2015-01-02
Answer
I looked at the website and datasheets of our anti-Ret Rabbit Monoclonal antibody and I see that M00293 has been validated on rat in both WB and IP. Thus M00293 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-01-02
