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Product Info Summary
| SKU: | M04136 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-p23 Monoclonal Antibody
SKU/Catalog Number
M04136
BM5211 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-p23 Monoclonal Antibody catalog # M04136. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p23 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04136)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACAH-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human p23 Molecular chaperone that localizes to genomic response elements in a hormone-dependent manner and disrupts receptor-mediated transcriptional activation, by promoting disassembly of transcriptional regulatory complexes.
Reactive Species
M04136 is reactive to PTGES3 in Human, Mouse, Rat
Observed Molecular Weight
23 kDa
Calculated molecular weight
18.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04136 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
IP 1:20
Positive Control
WB: HeLa cell, NIH/3T3 cell, PC12 cell
Validation Images & Assay Conditions
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Western blot analysis of p23 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate; (3) PC12 cell lysate.
Specific Publications For Anti-p23 Monoclonal Antibody (M04136)
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3 Customer Q&As for Anti-p23 Monoclonal Antibody
Question
Can you help my question with product M04136, anti-p23 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M04136 anti-p23 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-13
Question
Is this M04136 anti-p23 Monoclonal antibody reactive to the isotypes of PTGES3?
K. Dhar
Verified customer
Asked: 2017-08-31
Answer
The immunogen of M04136 anti-p23 Monoclonal antibody is A synthesized peptide derived from human p23 Molecular chaperone that localizes to genomic response elements in a hormone-dependent manner and disrupts receptor-mediated transcriptional activation, by promoting disassembly of transcriptional regulatory complexes. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-08-31
Question
Would anti-p23 Monoclonal antibody M04136 work for IF with cervix carcinoma?
T. Collins
Verified customer
Asked: 2013-03-01
Answer
According to the expression profile of cervix carcinoma, PTGES3 is highly expressed in cervix carcinoma. So, it is likely that anti-p23 Monoclonal antibody M04136 will work for IF with cervix carcinoma.
Boster Scientific Support
Answered: 2013-03-01
