Anti-Angiotensin Converting Enzyme 1/ACE Antibody Picoband™

ACE/CD143 antibody

Boster Bio Anti-Angiotensin Converting Enzyme 1/ACE Antibody Picoband™ catalog # PB9124. Tested in IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 4 publication(s).

Product Info Summary

SKU: PB9124
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC, IHC-F, ICC, WB

Product Name

Anti-Angiotensin Converting Enzyme 1/ACE Antibody Picoband™

View all ACE/CD143 Antibodies

SKU/Catalog Number

PB9124

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Angiotensin Converting Enzyme 1/ACE Antibody Picoband™ catalog # PB9124. Tested in IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Angiotensin Converting Enzyme 1/ACE Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9124)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human ACE recombinant protein (Position: K651-Y864). Human ACE shares 73% and 76% amino acid (aa) sequences identity with mouse and rat ACE, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9124 is reactive to ACE in Human, Mouse, Rat

Applications

PB9124 is guaranteed for IF, IHC, IHC-F, ICC, WB Boster Guarantee

Observed Molecular Weight

180 kDa

Calculated molecular weight

149.715kDa

Background of ACE/CD143

Angiotensin-converting enzyme (ACE), an exopeptidase, is a circulating enzyme that participates in the body's renin-angiotensin system (RAS), which mediates extracellular volume (i.e. that of the blood plasma, lymph and interstitial fluid), and arterial vasoconstriction. It is secreted by pulmonary and renal endothelial cells and catalyzes the conversion of decapeptide angiotensin I to octapeptide angiotensin II. Using a DNA marker at the growth hormone gene locus, which they characterized as 'extremely polymorphic' and which showed no recombination with ACE, ACE was mapped to 17q22-q24, consistent with the in situ hybridization mapping to 17q23. ACE, or kininase II, is a dipeptidyl carboxypeptidase that plays an important role in blood pressure regulation and electrolyte balance by hydrolyzing angiotensin I into angiotensin II, a potent vasopressor, and aldosterone-stimulating peptide. The enzyme is also able to inactivate bradykinin, a potent vasodilator.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For ACE (Source: Uniprot.org, NCBI)

Gene Name

ACE

Full Name

Angiotensin-converting enzyme

Weight

149.715kDa

Superfamily

peptidase M2 family

Alternative Names

ACE; ACE1angiotensin converting enzyme, somatic isoform; angiotensin I converting enzyme (peptidyl-dipeptidase A) 1; carboxycathepsin; CD143 antigen; CD143; DCP; DCP1; DCP1angiotensin-converting enzyme; dipeptidyl carboxypeptidase 1; Dipeptidyl carboxypeptidase I; EC 3.2.1.-; EC 3.4.15.1; Kininase II; MGC26566; MVCD3; peptidase P; testicular ECA ACE ACE1, CD143, DCP, DCP1 angiotensin I converting enzyme angiotensin-converting enzyme|CD143 antigen|angiotensin I converting enzyme (peptidyl-dipeptidase A) 1|carboxycathepsin|dipeptidyl carboxypeptidase 1|dipeptidyl carboxypeptidase I|kininase II|peptidase P

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ACE, check out the ACE Infographic

ACE infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ACE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9124 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Low concentrations of bilirubin inhibit activation of hepatic stellate cells in vitro

Local renin-angiotensin system is associated with bone mineral density of glucocorticoid-induced osteoporosis patients

Telmisartan protects chronic intermittent hypoxic mice via modulating cardiac renin-angiotensin system activity

Aliskiren has chondroprotective efficacy in a rat model of osteoarthritis through suppression of the local renin-angiotensin system

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5 Customer Q&As for Anti-Angiotensin Converting Enzyme 1/ACE Antibody Picoband™

Question

We are currently using anti-Angiotensin Converting Enzyme 1/ACE antibody PB9124 for rat tissue, and we are well pleased with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-14

Answer

The anti-Angiotensin Converting Enzyme 1/ACE antibody (PB9124) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-14

Question

Our lab used your anti-Angiotensin Converting Enzyme 1/ACE antibody for IHC-P on testis in the past. I am using human, and We want to use the antibody for IF next. you antibody examining testis as well as umbilical vein endothelial cell in our next experiment. Do you have any suggestion on which antibody would work the best for IF?

Verified Customer

Verified customer

Asked: 2019-07-18

Answer

I have checked the website and datasheets of our anti-Angiotensin Converting Enzyme 1/ACE antibody and it seems that PB9124 has been tested on human in both IHC-P and IF. Thus PB9124 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-07-18

Question

Our lab were well pleased with the WB result of your anti-Angiotensin Converting Enzyme 1/ACE antibody. However we have observed positive staining in lung angiotensin-converting enzyme using this antibody. Is that expected? Could you tell me where is ACE supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-07-04

Answer

Based on literature, lung does express ACE. Generally ACE expresses in angiotensin-converting enzyme, soluble form:, cell membrane. Regarding which tissues have ACE expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 16625196
Liver, Pubmed ID: 19159218
Lung, Pubmed ID: 2558109
Plasma, Pubmed ID: 16335952
Testis, Pubmed ID: 14702039
Umbilical vein endothelial cell, Pubmed ID: 9642152

Boster Scientific Support

Answered: 2019-07-04

Question

I am interested in using your anti-Angiotensin Converting Enzyme 1/ACE antibody for positive regulation of blood pressure studies. Has this antibody been tested with western blotting on mouse lung tissue? We would like to see some validation images before ordering.

C. Moore

Verified customer

Asked: 2016-08-17

Answer

Thanks for your inquiry. This PB9124 anti-Angiotensin Converting Enzyme 1/ACE antibody is validated on rat lung tissue, mouse lung tissue, human placenta tissue. It is guaranteed to work for IF, IHC-P, IHC-F, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2016-08-17

Question

We have seen staining in mouse testis. Are there any suggestions? Is anti-Angiotensin Converting Enzyme 1/ACE antibody supposed to stain testis positively?

E. Parker

Verified customer

Asked: 2015-01-13

Answer

From literature testis does express ACE. From Uniprot.org, ACE is expressed in testis, brain, lung, umbilical vein endothelial cell, plasma, liver, among other tissues. Regarding which tissues have ACE expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 16625196
Liver, Pubmed ID: 19159218
Lung, Pubmed ID: 2558109
Plasma, Pubmed ID: 16335952
Testis, Pubmed ID: 14702039
Umbilical vein endothelial cell, Pubmed ID: 9642152

Boster Scientific Support

Answered: 2015-01-13

Size

Conjugation

Total: $370

SKU:PB9124

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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