Product Info Summary
SKU: | PA2196-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Angiotensin Converting Enzyme 1/ACE Antibody
SKU/Catalog Number
PA2196-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Angiotensin Converting Enzyme 1/ACE Antibody catalog # PA2196-1. Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Angiotensin Converting Enzyme 1/ACE Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2196-1)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Angiotensin Converting Enzyme 1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2196-1 is reactive to ACE in Human
Applications
PA2196-1 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
150 kDa
Calculated molecular weight
149.715kDa
Background of ACE/CD143
Angiotensin I converting enzyme (ACE), also called DCP or CD143, is a zinc-containing dipeptidyl carboxypeptidase widely distributed in mammalian tissues and is though to play a critical role in blood pressure regulation. ACE gene is mapped to 17q23.3. This gene encodes an enzyme involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme or cardiovascular pathophysiologies.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Anti-ACE antibody, PA2196-1, IHC(P)
IHC(P): Human Placenta Tissue
Click image to see more details
Anti-ACE antibody, PA2196-1, IHC(P)
IHC(P): Human Prostatic Cancer Tissue
Click image to see more details
Anti-ACE antibody, PA2196-1, Western blotting
All lanes: Anti Angiotensin Converting Enzyme 1 (PA2196-1) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: 22RV1 Whole Cell Lysate at 40ug
Predicted bind size: 150KD
Observed bind size: 150KD
Protein Target Info & Infographic
Gene/Protein Information For ACE (Source: Uniprot.org, NCBI)
Gene Name
ACE
Full Name
Angiotensin-converting enzyme
Weight
149.715kDa
Superfamily
peptidase M2 family
Alternative Names
ACE; ACE1angiotensin converting enzyme, somatic isoform; angiotensin I converting enzyme (peptidyl-dipeptidase A) 1; carboxycathepsin; CD143 antigen; CD143; DCP; DCP1; DCP1angiotensin-converting enzyme; dipeptidyl carboxypeptidase 1; Dipeptidyl carboxypeptidase I; EC 3.2.1.-; EC 3.4.15.1; Kininase II; MGC26566; MVCD3; peptidase P; testicular ECA ACE ACE1, CD143, DCP, DCP1 angiotensin I converting enzyme angiotensin-converting enzyme|CD143 antigen|angiotensin I converting enzyme (peptidyl-dipeptidase A) 1|carboxycathepsin|dipeptidyl carboxypeptidase 1|dipeptidyl carboxypeptidase I|kininase II|peptidase P
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ACE, check out the ACE Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ACE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Angiotensin Converting Enzyme 1/ACE Antibody (PA2196-1)
Hello CJ!
PA2196-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Aliskiren has chondroprotective efficacy in a rat model of osteoarthritis through suppression of the local renin-angiotensin system
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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16 Customer Q&As for Anti-Angiotensin Converting Enzyme 1/ACE Antibody
Question
Does PA2196-1 anti-Angiotensin Converting Enzyme 1/ACE antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
It shows on the product datasheet, PA2196-1 anti-Angiotensin Converting Enzyme 1/ACE antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-21
Question
We are currently using anti-Angiotensin Converting Enzyme 1/ACE antibody PA2196-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
The anti-Angiotensin Converting Enzyme 1/ACE antibody (PA2196-1) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-24
Question
Here is the WB image, lot number and protocol we used for lung using anti-Angiotensin Converting Enzyme 1/ACE antibody PA2196-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-11
Question
I have a question about product PA2196-1, anti-Angiotensin Converting Enzyme 1/ACE antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2196-1 anti-Angiotensin Converting Enzyme 1/ACE antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-02
Question
We have seen staining in human lung. What should we do? Is anti-Angiotensin Converting Enzyme 1/ACE antibody supposed to stain lung positively?
Verified Customer
Verified customer
Asked: 2019-12-10
Answer
From what I have seen in literature lung does express ACE. From what I have seen in Uniprot.org, ACE is expressed in testis, brain, lung, umbilical vein endothelial cell, plasma, liver, among other tissues. Regarding which tissues have ACE expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 16625196
Liver, Pubmed ID: 19159218
Lung, Pubmed ID: 2558109
Plasma, Pubmed ID: 16335952
Testis, Pubmed ID: 14702039
Umbilical vein endothelial cell, Pubmed ID: 9642152
Boster Scientific Support
Answered: 2019-12-10
Question
Is this PA2196-1 anti-Angiotensin Converting Enzyme 1/ACE antibody reactive to the isotypes of ACE?
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
The immunogen of PA2196-1 anti-Angiotensin Converting Enzyme 1/ACE antibody is A synthetic peptide corresponding to a sequence in the middle region of human Angiotensin Converting Enzyme 1(702-715aa TQARKFDVNQLQNT). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-20
Question
We were content with the WB result of your anti-Angiotensin Converting Enzyme 1/ACE antibody. However we have seen positive staining in liver soluble form: using this antibody. Is that expected? Could you tell me where is ACE supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
According to literature, liver does express ACE. Generally ACE expresses in angiotensin-converting enzyme, soluble form:, cell membrane. Regarding which tissues have ACE expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 16625196
Liver, Pubmed ID: 19159218
Lung, Pubmed ID: 2558109
Plasma, Pubmed ID: 16335952
Testis, Pubmed ID: 14702039
Umbilical vein endothelial cell, Pubmed ID: 9642152
Boster Scientific Support
Answered: 2019-11-04
Question
I see that the anti-Angiotensin Converting Enzyme 1/ACE antibody PA2196-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-28
Question
My question regards using your anti-Angiotensin Converting Enzyme 1/ACE antibody for hormone catabolic process studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-05-16
Answer
I appreciate your inquiry. This PA2196-1 anti-Angiotensin Converting Enzyme 1/ACE antibody is tested on human placenta tissue, prostatic cancer tissue, hela whole cell lysate, a549 whole cell lysate, 22rv1 whole cell lysate. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-05-16
Question
Is a blocking peptide available for product anti-Angiotensin Converting Enzyme 1/ACE antibody (PA2196-1)?
Verified Customer
Verified customer
Asked: 2018-03-14
Answer
We do provide the blocking peptide for product anti-Angiotensin Converting Enzyme 1/ACE antibody (PA2196-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-03-14
Question
Would anti-Angiotensin Converting Enzyme 1/ACE antibody PA2196-1 work for WB with lung?
Verified Customer
Verified customer
Asked: 2017-11-10
Answer
According to the expression profile of lung, ACE is highly expressed in lung. So, it is likely that anti-Angiotensin Converting Enzyme 1/ACE antibody PA2196-1 will work for WB with lung.
Boster Scientific Support
Answered: 2017-11-10
Question
Is there a BSA free version of anti-Angiotensin Converting Enzyme 1/ACE antibody PA2196-1 available?
Verified Customer
Verified customer
Asked: 2017-07-05
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Angiotensin Converting Enzyme 1/ACE antibody PA2196-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-07-05
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-Angiotensin Converting Enzyme 1/ACE antibody PA2196-1. Let me know if you need anything else.
M. Mangal
Verified customer
Asked: 2017-01-12
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-01-12
Question
We bought anti-Angiotensin Converting Enzyme 1/ACE antibody for IHC on lung in the past. I am using human, and We intend to use the antibody for WB next. We want examining lung as well as testis in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
E. Anderson
Verified customer
Asked: 2014-09-08
Answer
I looked at the website and datasheets of our anti-Angiotensin Converting Enzyme 1/ACE antibody and it appears that PA2196-1 has been tested on human in both IHC and WB. Thus PA2196-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-09-08
Question
I was wanting to use your anti-Angiotensin Converting Enzyme 1/ACE antibody for WB for human lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human lung identification?
R. Wu
Verified customer
Asked: 2014-07-30
Answer
As indicated on the product datasheet, PA2196-1 anti-Angiotensin Converting Enzyme 1/ACE antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-07-30
Question
We are interested in to test anti-Angiotensin Converting Enzyme 1/ACE antibody PA2196-1 on human lung for research purposes, then I may be interested in using anti-Angiotensin Converting Enzyme 1/ACE antibody PA2196-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
O. Anderson
Verified customer
Asked: 2013-06-27
Answer
The products we sell, including anti-Angiotensin Converting Enzyme 1/ACE antibody PA2196-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-06-27