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Product Info Summary
| SKU: | A05087-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-ACSL5 Antibody Picoband®
SKU/Catalog Number
A05087-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ACSL5 Antibody Picoband® catalog # A05087-2. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ACSL5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05087-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human ACSL5, different from the related mouse and rat sequences by six amino acids.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05087-2 is reactive to ACSL5 in Human, Mouse, Rat
Observed Molecular Weight
76 kDa
Calculated molecular weight
76.0 kDa
Background of ACSL5
Long-chain-fatty-acid—CoA ligase 5 is an enzyme that in humans is encoded by the ACSL5 gene. The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in uterus and spleen, and in trace amounts in normal brain, but has markedly increased levels in malignant gliomas. This gene functions in mediating fatty acid-induced glioma cell growth. Three transcript variants encoding two different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05087-2 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: rat liver tissue, mouse liver tissue
FCM: HepG2 cell
Validation Images & Assay Conditions
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Western blot analysis of ACSL5 using anti-ACSL5 antibody (A05087-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACSL5 antigen affinity purified polyclonal antibody (Catalog # A05087-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACSL5 at approximately 76 kDa. The expected band size for ACSL5 is at 76 kDa.
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Flow Cytometry analysis of HepG2 cells using anti-ACSL5 antibody (A05087-2).
Overlay histogram showing HepG2 cells stained with A05087-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACSL5 Antibody (A05087-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Specific Publications For Anti-ACSL5 Antibody Picoband® (A05087-2)
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3 Customer Q&As for Anti-ACSL5 Antibody Picoband®
Question
We are currently using anti-ACSL5 antibody A05087-2 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
The anti-ACSL5 antibody (A05087-2) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-25
Question
I was wanting to use your anti-ACSL5 antibody for WB for human small intestine mucosa on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human small intestine mucosa identification?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
It shows on the product datasheet, A05087-2 anti-ACSL5 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human small intestine mucosa in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-17
Question
I would like to test anti-ACSL5 antibody A05087-2 on human small intestine mucosa for research purposes, then I may be interested in using anti-ACSL5 antibody A05087-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
E. Brown
Verified customer
Asked: 2018-04-10
Answer
The products we sell, including anti-ACSL5 antibody A05087-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-04-10
