Anti-ACSL5 Antibody Picoband™

ACSL5 antibody

Boster Bio Anti-ACSL5 Antibody Picoband™ catalog # A05087-2. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A05087-2
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, WB

Product Name

Anti-ACSL5 Antibody Picoband™

View all ACSL5 Antibodies

SKU/Catalog Number

A05087-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ACSL5 Antibody Picoband™ catalog # A05087-2. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ACSL5 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05087-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human ACSL5, different from the related mouse and rat sequences by six amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A05087-2 is reactive to ACSL5 in Human, Mouse, Rat

Applications

A05087-2 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

76 kDa

Calculated molecular weight

75.991kDa

Background of ACSL5

Long-chain-fatty-acid—CoA ligase 5 is an enzyme that in humans is encoded by the ACSL5 gene. The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in uterus and spleen, and in trace amounts in normal brain, but has markedly increased levels in malignant gliomas. This gene functions in mediating fatty acid-induced glioma cell growth. Three transcript variants encoding two different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For ACSL5 (Source: Uniprot.org, NCBI)

Gene Name

ACSL5

Full Name

Long-chain-fatty-acid--CoA ligase 5

Weight

75.991kDa

Superfamily

ATP-dependent AMP-binding enzyme family

Alternative Names

ACS2; ACS5FACL5 for fatty acid coenzyme A ligase 5; acyl-CoA synthetase long-chain family member 5; EC 6.2.1; EC 6.2.1.3; FACL5fatty acid coenzyme A ligase 5; fatty-acid-Coenzyme A ligase, long-chain 5; LACS 5; Long-chain acyl-CoA synthetase 5; long-chain fatty acid coenzyme A ligase 5; long-chain-fatty-acid--CoA ligase 5 ACSL5 ACS2, ACS5, FACL5 acyl-CoA synthetase long chain family member 5 long-chain-fatty-acid--CoA ligase 5|FACL5 for fatty acid coenzyme A ligase 5|LACS 5|arachidonate--CoA ligase|fatty acid coenzyme A ligase 5|fatty-acid-Coenzyme A ligase, long-chain 5|long-chain acyl-CoA synthetase 5|long-chain fatty acid coenzyme A ligase 5

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ACSL5, check out the ACSL5 Infographic

ACSL5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ACSL5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-ACSL5 Antibody Picoband™

Question

We are currently using anti-ACSL5 antibody A05087-2 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2020-02-25

Answer

The anti-ACSL5 antibody (A05087-2) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-02-25

Question

I was wanting to use your anti-ACSL5 antibody for WB for human small intestine mucosa on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human small intestine mucosa identification?

Verified Customer

Verified customer

Asked: 2020-02-17

Answer

It shows on the product datasheet, A05087-2 anti-ACSL5 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human small intestine mucosa in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-02-17

Question

I would like to test anti-ACSL5 antibody A05087-2 on human small intestine mucosa for research purposes, then I may be interested in using anti-ACSL5 antibody A05087-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

E. Brown

Verified customer

Asked: 2018-04-10

Answer

The products we sell, including anti-ACSL5 antibody A05087-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-04-10

Size

Conjugation

Total: $370

SKU:A05087-2

In stock, 3+ left.

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A05087-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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