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recombinant

ACTA1 antibody (M02014-4) performs well in Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.

  • Validated for inter-lot consistency, specificity, and reproducibility
  • Committed to quality since 1993
  • Over 1 Publication
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m02014 4 if_1

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Immunofluorescent analysis of Hela cells, using Actin (Alpha-Actin) Antibody .
m02014 4 acta1 primary antibodies ihc testing 1m02014 4 acta1 primary antibodies ihc testing 2m02014 4 acta1 primary antibodies ihc testing 3m02014 4 acta1 primary antibodies ihc testing 4m02014 4 wb_1m02014 4 if_1

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Anti-Actin (Alpha-Actin) ACTA1 Rabbit Monoclonal Antibody

ACTA1 antibody

1 Publication
Reactive SpeciesHostApplication
Human, Mouse, RatRabbitFlow Cytometry, IP, IF, IHC, ICC, WB

SKU: M02014-4

Size: 100 μl

Bundle to save: I do not want free secondary ab
Price:
$370/100 μl

2 in stock. Ships Next Business Day.

Quantity: 1

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Overview

Tested Applications

ACTA1 antibody was positively detected by WB in:

A431 cell, MCF-7 cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

ACTA1 antibody was positively detected by IHC in:

mouse muscle tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

ACTA1 antibody was positively detected by ICC/IF in:

Hela cell

Recommend Dilution

Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20

* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.

Reactive Data

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Boster Guarantee:What is this?
IHC
IF
IP
FC
Mouse
Tested
Recommend
Recommend
Recommend
Human
Recommend
Recommend
Rat
Recommend
Recommend

Product Information

Product Name
Anti-Actin (Alpha-Actin) ACTA1 Rabbit Monoclonal Antibody
View all ACTA1 Antibodies
Form
Liquid
Uniprot
P68133
Gene ID
58
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
C-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Actin (Alpha-Actin)
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
42 kDa
Calculated Molecular Weight

42.1 kDa

Cite This Product
Anti-Actin (Alpha-Actin) ACTA1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02014-4)

Protocols

Western Blot protocol for ACTA1 antibody
Review Protocol
Immunohistochemistry (IHC) protocol for ACTA1 antibody
Review Protocol
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for ACTA1 antibody
Review Protocol

Publications

Application:
Species:
Dexmedetomidine attenuates H2O2-induced neonatal rat cardiomyocytes apoptosis through mitochondria- and ER-medicated oxidative stress pathways
Published: 2018-03-15
Journal: Molecular Medicine Reports
Application: IF
Impact Factor: 1.92
Species: Rat
PMID: 29568958
View Article

Review

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Product Promise

We promise Anti-Actin (Alpha-Actin) ACTA1 Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more

In stock
Order Product
M02014-4
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

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Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.

This product is for Research Use Only. Not for use in diagnostic procedures.

Update date: Oct 3, 2026