Anti-Actin (Alpha-Actin) ACTA1 Rabbit Monoclonal Antibody

ACTA1 antibody

Boster Bio Anti-Actin (Alpha-Actin) ACTA1 Rabbit Monoclonal Antibody catalog # M02014-4. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. Cited in 8 publication(s).

Product Info Summary

SKU: M02014-4
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IP, IF, IHC, ICC, WB

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Product Name

Anti-Actin (Alpha-Actin) ACTA1 Rabbit Monoclonal Antibody

View all ACTA1 Antibodies

SKU/Catalog Number

M02014-4

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Actin (Alpha-Actin) ACTA1 Rabbit Monoclonal Antibody catalog # M02014-4. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Actin (Alpha-Actin) ACTA1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02014-4)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

C-1

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Actin (Alpha-Actin)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M02014-4 is reactive to ACTA1 in Human, Mouse, Rat

Applications

M02014-4 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

66 kDa

Calculated molecular weight

42.051kDa

Background of ACTA1

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For ACTA1 (Source: Uniprot.org, NCBI)

Gene Name

ACTA1

Full Name

Actin, alpha skeletal muscle

Weight

42.051kDa

Superfamily

actin family

Alternative Names

ACTA; Actin; actin, alpha 1, skeletal muscle; alpha skeletal muscle actin; alpha skeletal muscle; alpha-actin-1; ASMA; CFTD; CFTDM; MPFD; NEM1; NEM2; NEM3 ACTA1 ACTA, ASMA, CFTD, CFTD1, CFTDM, MPFD, NEM1, NEM2, NEM3, SHPM actin alpha 1, skeletal muscle actin, alpha skeletal muscle|nemaline myopathy type 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ACTA1, check out the ACTA1 Infographic

ACTA1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ACTA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M02014-4 has been cited in 8 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Shen X,Zhang J,Zhang X,Wang Y,Hu Y,Guo J.Retinoic Acid-Induced Protein 14 (RAI14) Promotes mTOR-Mediated Inflammation Under Inflammatory Stress and Chemical Hypoxia in a U87 Glioblastoma Cell Line.Cell Mol Neurobiol.2019 Mar;39(2):241-254.doi:10.1007/s10571-018-0644-z.Epub 2018 Dec 15.PMID:30554401.
Species: Human
M02014-4 usage in article: APP:WB, SAMPLE:HUVECS, DILUTION:1:300

Cheng J,Luan J,Chen P,Kuang X,Jiang P,Zhang R,Chen S,Cheng F,Gou X.Immunosuppressive receptor LILRB1 acts as a potential regulator in hepatocellular carcinoma by integrating with SHP1.Cancer Biomark.2020; 28(3):309-319.doi:10.3233/CBM-190940.PMID:32390601
Species: Human
M02014-4 usage in article: APP:WB, SAMPLE:LO2,MHCC97L,HUH 7 AND HEPG2 CELLS, DILUTION:NA

Sandulescu T, Weniger J, Philippou S, Mücke T, Naumova EA, Arnold WH. Immunohistochemical evidence of striated muscle cells within midfacial superficial musculoaponeurotic system. Ann Anat. 2020 Nov 19:151647. doi: 10.1016/j.aanat.2020.151647. Epub ahead
Species: Human
M02014-4 usage in article: APP:IHC, SAMPLE:SMAS FIBROUS SEPTA, DILUTION:NA

Expression changes of ionic channels in early phase of cultured rat atrial myocytes induced by rapid pacing

The Effect of 5?-Adenylic Acid on Hepatic Proteome of Mice Radiated by 60Co ?-ray

Inhibition of human La protein by RNA interference downregulates hepatitis B virus mRNA in 2.2.15 cells

Engineered myocardial tissues constructed in vivo using cardiomyocyte-like cells derived from bone marrow mesenchymal stem cells in rats

Li Ys, Chen Bf, Li Xj, Zhang Wk, Tang Hb. Plos One. 2014 Sep 24;9(9):E107361. Doi: 10.1371/Journal.Pone.0107361. Ecollection 2014. Cytotoxicity Of Polyaniline Nanomaterial On Rat Celiac Macrophages In Vitro.

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16 Customer Q&As for Anti-Actin (Alpha-Actin) ACTA1 Rabbit Monoclonal Antibody

Question

I would like using your anti-Actin (Alpha-Actin) Rabbit Monoclonal antibody for skeletal muscle fiber adaptation studies. Has this antibody been tested with western blotting on a431 cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-03-24

Answer

I appreciate your inquiry. This M02014-4 anti-Actin (Alpha-Actin) Rabbit Monoclonal antibody is validated on a431 cell lysate, hela cells. It is guaranteed to work for Flow Cytometry, IP, IF, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-03-24

Question

We were well pleased with the WB result of your anti-Actin (Alpha-Actin) Rabbit Monoclonal antibody. However we have been able to see positive staining in biceps brachii cytoskeleton. using this antibody. Is that expected? Could you tell me where is ACTA1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-02-21

Answer

From literature, biceps brachii does express ACTA1. Generally ACTA1 expresses in cytoplasm, cytoskeleton. Regarding which tissues have ACTA1 expression, here are a few articles citing expression in various tissues:
Skeletal muscle, Pubmed ID: 6190133, 15489334

Boster Scientific Support

Answered: 2020-02-21

Question

Do you have a BSA free version of anti-Actin (Alpha-Actin) Rabbit Monoclonal antibody M02014-4 available?

Verified Customer

Verified customer

Asked: 2020-02-07

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Actin (Alpha-Actin) Rabbit Monoclonal antibody M02014-4 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-02-07

Question

Is this M02014-4 anti-Actin (Alpha-Actin) Rabbit Monoclonal antibody reactive to the isotypes of ACTA1?

V. Parker

Verified customer

Asked: 2020-02-05

Answer

The immunogen of M02014-4 anti-Actin (Alpha-Actin) Rabbit Monoclonal antibody is A synthesized peptide derived from human Actin (Alpha-Actin). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-02-05

Question

Would anti-Actin (Alpha-Actin) Rabbit Monoclonal antibody M02014-4 work for IP with biceps brachii?

Verified Customer

Verified customer

Asked: 2020-01-14

Answer

According to the expression profile of biceps brachii, ACTA1 is highly expressed in biceps brachii. So, it is likely that anti-Actin (Alpha-Actin) Rabbit Monoclonal antibody M02014-4 will work for IP with biceps brachii.

Boster Scientific Support

Answered: 2020-01-14

Question

See below the WB image, lot number and protocol we used for biceps brachii using anti-Actin (Alpha-Actin) Rabbit Monoclonal antibody M02014-4. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-08-16

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-16

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for biceps brachii using anti-Actin (Alpha-Actin) Rabbit Monoclonal antibody M02014-4. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-06-19

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-19

Question

I have a question about product M02014-4, anti-Actin (Alpha-Actin) Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

M. Roberts

Verified customer

Asked: 2019-06-05

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02014-4 anti-Actin (Alpha-Actin) Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-06-05

Question

I was wanting to use your anti-Actin (Alpha-Actin) Rabbit Monoclonal antibody for IP for rat biceps brachii on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat biceps brachii identification?

Verified Customer

Verified customer

Asked: 2019-04-26

Answer

As indicated on the product datasheet, M02014-4 anti-Actin (Alpha-Actin) Rabbit Monoclonal antibody has been tested for Flow Cytometry, IP, IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat biceps brachii in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-04-26

Question

I see that the anti-Actin (Alpha-Actin) Rabbit Monoclonal antibody M02014-4 works with IP, what is the protocol used to produce the result images on the product page?

O. Krishna

Verified customer

Asked: 2019-01-04

Answer

You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-01-04

Question

We have seen staining in rat biceps brachii. Are there any suggestions? Is anti-Actin (Alpha-Actin) Rabbit Monoclonal antibody supposed to stain biceps brachii positively?

Verified Customer

Verified customer

Asked: 2017-12-18

Answer

From what I have seen in literature biceps brachii does express ACTA1. From what I have seen in Uniprot.org, ACTA1 is expressed in biceps brachii, skeletal muscle, among other tissues. Regarding which tissues have ACTA1 expression, here are a few articles citing expression in various tissues:
Skeletal muscle, Pubmed ID: 6190133, 15489334

Boster Scientific Support

Answered: 2017-12-18

Question

We are currently using anti-Actin (Alpha-Actin) Rabbit Monoclonal antibody M02014-4 for mouse tissue, and we are well pleased with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?

E. Yang

Verified customer

Asked: 2017-12-12

Answer

The anti-Actin (Alpha-Actin) Rabbit Monoclonal antibody (M02014-4) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-12-12

Question

Would M02014-4 anti-Actin (Alpha-Actin) Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-09-13

Answer

As indicated on the product datasheet, M02014-4 anti-Actin (Alpha-Actin) Rabbit Monoclonal antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-09-13

Question

We purchased anti-Actin (Alpha-Actin) Rabbit Monoclonal antibody for IHC on skeletal muscle last year. I am using mouse, and We are going to use the antibody for Flow Cytometry next. Our lab want to know about examining skeletal muscle as well as biceps brachii in our next experiment. Could you please give me some suggestion on which antibody would work the best for Flow Cytometry?

L. Baker

Verified customer

Asked: 2016-09-15

Answer

I looked at the website and datasheets of our anti-Actin (Alpha-Actin) Rabbit Monoclonal antibody and it appears that M02014-4 has been tested on mouse in both IHC and Flow Cytometry. Thus M02014-4 should work for your application. Our Boster satisfaction guarantee will cover this product for Flow Cytometry in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for Flow Cytometry detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2016-09-15

Question

Is a blocking peptide available for product anti-Actin (Alpha-Actin) Rabbit Monoclonal antibody (M02014-4)?

B. Johnson

Verified customer

Asked: 2015-04-28

Answer

We do provide the blocking peptide for product anti-Actin (Alpha-Actin) Rabbit Monoclonal antibody (M02014-4). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2015-04-28

Question

My question regards to test anti-Actin (Alpha-Actin) Rabbit Monoclonal antibody M02014-4 on rat biceps brachii for research purposes, then I may be interested in using anti-Actin (Alpha-Actin) Rabbit Monoclonal antibody M02014-4 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

B. Williams

Verified customer

Asked: 2015-04-16

Answer

The products we sell, including anti-Actin (Alpha-Actin) Rabbit Monoclonal antibody M02014-4, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2015-04-16

Order DetailsPrice
M02014-4

100μL

$370
M02014-4-30ul

30μL sample

$99
M02014-4-carrier-free

Carrier Free

$370

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M02014-4
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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