Anti-Prosurfactant Protein B/SFTPB Antibody Picoband®

Product Name

Anti-Prosurfactant Protein B/SFTPB Antibody Picoband®

View all SFTPB Antibodies

SKU/Catalog Number

A03441-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Prosurfactant Protein B/SFTPB Antibody Picoband® catalog # A03441-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Prosurfactant Protein B/SFTPB Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03441-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human SFTPB, which shares 80% amino acid (aa) sequence identity with both mouse and rat SFTPB.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03441-1 is reactive to SFTPB in Human, Mouse, Rat

Observed Molecular Weight

46 kDa

Calculated molecular weight

42.1 kDa

Background of SFTPB

Pulmonary surfactant-associated protein B is a protein that in humans is encoded by the SFTPB gene. This gene encodes the pulmonary-associated surfactant protein B (SPB), an amphipathic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. The SPB enhances the rate of spreading and increases the stability of surfactant monolayers in vitro. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 1, also called pulmonary alveolar proteinosis due to surfactant protein B deficiency, and are associated with fatal respiratory distress in the neonatal period. Alternatively spliced transcript variants encoding the same protein have been identified.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A03441-1 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml

Positive Control

WB: human MCF-7 whole cell, human COLO-320 whole cell, human HepG2 whole cell

Validation Images & Assay Conditions

See full target information SFTPB

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