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Product Info Summary
| SKU: | M02014-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Actin ACTA1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02014-1
BM3872 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Actin ACTA1 Rabbit Monoclonal Antibody catalog # M02014-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Actin ACTA1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02014-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
BE-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Actin
Reactive Species
M02014-1 is reactive to ACTA1 in Human, Mouse, Rat
Observed Molecular Weight
42 kDa
Calculated molecular weight
42.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02014-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-5000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20
Positive Control
WB: human HepG2 whole cell, human Caco-2 whole cell, human 293T whole cell, human SH-SY5Y whole cell, rat heart tissue, rat small intestine tissue, mouse heart tissue, mouse small intestine tissue
IHC: mouse heart tissue
Validation Images & Assay Conditions
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Western blot analysis of ACTA1 using anti-ACTA1 antibody (M02014-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human SH-SY5Y whole cell lysates,
Lane 5: rat heart tissue lysates,
Lane 6: rat small intestine tissue lysates,
Lane 7: mouse heart tissue lysates,
Lane 8: mouse small intestine tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACTA1 antigen affinity purified monoclonal antibody (Catalog # M02014-1) at 1:3000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACTA1 at approximately 42 kDa. The expected band size for ACTA1 is at 42 kDa.
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Immunohistochemical analysis of paraffin-embedded mouse heart, using Actin Antibody.
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Immunofluorescent analysis of Hela cells, using Actin Antibody.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Specific Publications For Anti-Actin ACTA1 Rabbit Monoclonal Antibody (M02014-1)
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Customer Q&As
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16 Customer Q&As for Anti-Actin ACTA1 Rabbit Monoclonal Antibody
Question
My team were content with the WB result of your anti-Actin Rabbit Monoclonal antibody. However we have observed positive staining in skeletal muscle cytoplasm using this antibody. Is that expected? Could you tell me where is ACTA1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
From literature, skeletal muscle does express ACTA1. Generally ACTA1 expresses in cytoplasm, cytoskeleton. Regarding which tissues have ACTA1 expression, here are a few articles citing expression in various tissues:
Skeletal muscle, Pubmed ID: 6190133, 15489334
Boster Scientific Support
Answered: 2020-04-16
Question
I see that the anti-Actin Rabbit Monoclonal antibody M02014-1 works with ICC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-04-07
Question
We have observed staining in human biceps brachii. What should we do? Is anti-Actin Rabbit Monoclonal antibody supposed to stain biceps brachii positively?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
From literature biceps brachii does express ACTA1. From Uniprot.org, ACTA1 is expressed in biceps brachii, skeletal muscle, among other tissues. Regarding which tissues have ACTA1 expression, here are a few articles citing expression in various tissues:
Skeletal muscle, Pubmed ID: 6190133, 15489334
Boster Scientific Support
Answered: 2020-01-08
Question
I would like to test anti-Actin Rabbit Monoclonal antibody M02014-1 on human skeletal muscle for research purposes, then I may be interested in using anti-Actin Rabbit Monoclonal antibody M02014-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
K. Krishna
Verified customer
Asked: 2020-01-01
Answer
The products we sell, including anti-Actin Rabbit Monoclonal antibody M02014-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-01
Question
Will anti-Actin Rabbit Monoclonal antibody M02014-1 work for ICC with skeletal muscle?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
According to the expression profile of skeletal muscle, ACTA1 is highly expressed in skeletal muscle. So, it is likely that anti-Actin Rabbit Monoclonal antibody M02014-1 will work for ICC with skeletal muscle.
Boster Scientific Support
Answered: 2019-12-06
Question
I was wanting to use your anti-Actin Rabbit Monoclonal antibody for ICC for human skeletal muscle on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human skeletal muscle identification?
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
You can see on the product datasheet, M02014-1 anti-Actin Rabbit Monoclonal antibody has been tested for Flow Cytometry, IP, IF, IHC, ICC, WB on chicken, human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human skeletal muscle in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-22
Question
Do you have a BSA free version of anti-Actin Rabbit Monoclonal antibody M02014-1 available?
Verified Customer
Verified customer
Asked: 2019-09-06
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Actin Rabbit Monoclonal antibody M02014-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-06
Question
My question regarding product M02014-1, anti-Actin Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-08-17
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02014-1 anti-Actin Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-08-17
Question
Is a blocking peptide available for product anti-Actin Rabbit Monoclonal antibody (M02014-1)?
Verified Customer
Verified customer
Asked: 2018-05-08
Answer
We do provide the blocking peptide for product anti-Actin Rabbit Monoclonal antibody (M02014-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-05-08
Question
We are currently using anti-Actin Rabbit Monoclonal antibody M02014-1 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says chicken, human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-05
Answer
The anti-Actin Rabbit Monoclonal antibody (M02014-1) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-05
Question
We bought anti-Actin Rabbit Monoclonal antibody for ICC on skeletal muscle in the past. I am using mouse, and We are going to use the antibody for WB next. I would like examining skeletal muscle as well as biceps brachii in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
A. Roberts
Verified customer
Asked: 2017-10-12
Answer
I looked at the website and datasheets of our anti-Actin Rabbit Monoclonal antibody and it appears that M02014-1 has been validated on mouse in both ICC and WB. Thus M02014-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-10-12
Question
Will M02014-1 anti-Actin Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
J. Singh
Verified customer
Asked: 2016-08-09
Answer
You can see on the product datasheet, M02014-1 anti-Actin Rabbit Monoclonal antibody as been tested on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-08-09
Question
I have attached the WB image, lot number and protocol we used for skeletal muscle using anti-Actin Rabbit Monoclonal antibody M02014-1. Please let me know if you require anything else.
T. Dhar
Verified customer
Asked: 2016-07-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-07-08
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skeletal muscle using anti-Actin Rabbit Monoclonal antibody M02014-1. Let me know if you need anything else.
L. Roberts
Verified customer
Asked: 2015-08-21
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-08-21
Question
My question regards using your anti-Actin Rabbit Monoclonal antibody for striated muscle contraction studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
O. Patel
Verified customer
Asked: 2014-06-17
Answer
Thanks for your inquiry. This M02014-1 anti-Actin Rabbit Monoclonal antibody is tested on hela cells. It is guaranteed to work for Flow Cytometry, IP, IF, IHC, ICC, WB in chicken, human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2014-06-17
Question
Is this M02014-1 anti-Actin Rabbit Monoclonal antibody reactive to the isotypes of ACTA1?
F. Yang
Verified customer
Asked: 2014-01-30
Answer
The immunogen of M02014-1 anti-Actin Rabbit Monoclonal antibody is A synthesized peptide derived from human Actin. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-01-30
