Anti-ACTN3 Antibody Picoband™

Alpha Actinin 3 antibody

Boster Bio Anti-ACTN3 Antibody Picoband™ catalog # PB10026. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: PB10026
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IHC, WB

Product Name

Anti-ACTN3 Antibody Picoband™

View all Alpha Actinin 3 Antibodies

SKU/Catalog Number

PB10026

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ACTN3 Antibody Picoband™ catalog # PB10026. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ACTN3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10026)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human ACTN3, different from the related mouse sequence by five amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB10026 is reactive to ACTN3 in Human, Mouse, Rat

Applications

PB10026 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee

Observed Molecular Weight

103 kDa

Calculated molecular weight

103.241kDa

Background of Alpha Actinin 3

Alpha-actinin-3, also known as alpha-actinin skeletal muscle isoform 3 or F-actin cross-linking protein, is a protein that in humans is encoded by the ACTN3 gene. This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For ACTN3 (Source: Uniprot.org, NCBI)

Gene Name

ACTN3

Full Name

Alpha-actinin-3

Weight

103.241kDa

Superfamily

alpha-actinin family

Alternative Names

actinin, alpha 3; Alpha-actinin skeletal muscle isoform 3; alpha-actinin skeletal muscle; alpha-actinin-3; F-actin cross-linking protein; MGC117002; MGC117005 ACTN3 ACTN3D actinin alpha 3 alpha-actinin-3|F-actin cross-linking protein|alpha-actinin skeletal muscle

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ACTN3, check out the ACTN3 Infographic

ACTN3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ACTN3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB10026 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Protein profile of fiber types in human skeletal muscle: a single-fiber proteomics study

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1 Customer Q&As for Anti-ACTN3 Antibody Picoband™

Question

We are currently using anti-ACTN3 antibody PB10026 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2018-09-26

Answer

The anti-ACTN3 antibody (PB10026) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-09-26

Size

Conjugation

Total: $370

SKU:PB10026

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PB10026
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

Troubleshooting

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