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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
|Application:||Flow Cytometry, IF, IHC, ICC, WB|
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Anti-POMT2 Antibody Picoband™
Boster Bio Anti-POMT2 Antibody Picoband™ catalog # A04876-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-POMT2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04876-1)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
A synthetic peptide corresponding to a sequence of human POMT2 (QAARAAGRDVAAEAVARSPKR).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A04876-1 is reactive to POMT2 in Human, Mouse, Rat
A04876-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Background of POMT2
Protein O-mannosyl-transferase 2 is an enzyme that in humans is encoded by the POMT2 gene. This gene is mapped to chromosome 14q24.3. The protein encoded by this gene is an O-mannosyltransferase that requires interaction with the product of the POMT1 gene for enzymatic function. The encoded protein is found in the membrane of the endoplasmic reticulum. Defects in this gene are a cause of Walker-Warburg syndrome (WWS).
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat
Flow Cytometry, 1-3μg/1x106 cells
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of POMT2 using anti-POMT2 antibody (A04876-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: rat lung tissue lysates,
Lane 5: rat testis tissue lysates,
Lane 6: rat spleen tissue lysates,
Lane 7: mouse lung tissue lysates,
Lane 8: mouse testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POMT2 antigen affinity purified polyclonal antibody (Catalog # A04876-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POMT2 at approximately 84KD. The expected band size for POMT2 is at 84KD.
Gene/Protein Information For POMT2 (Source: Uniprot.Org, NCBI)
Protein O-mannosyl-transferase 2
glycosyltransferase 39 family
DKFZp686G10254; Dolichyl-phosphate-mannose--protein mannosyltransferase 2; EC 184.108.40.206; FLJ22309; MDDGA2; MDDGB2; MDDGC2; protein O-mannosyl-transferase 2; protein-O-mannosyltransferase 2 POMT2 LGMD2N, LGMDR14, MDDGA2, MDDGB2, MDDGC2 protein O-mannosyltransferase 2 protein O-mannosyl-transferase 2|dolichyl-phosphate-mannose--protein mannosyltransferase 2*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on POMT2, check out the POMT2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for POMT2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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