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Product Info Summary
| SKU: | M11888 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Monkey |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-ACY3 Mouse Monoclonal Antibody [Clone ID: OTI2D5]
SKU/Catalog Number
M11888
Size
100 µl
Description
Boster Bio ACY3 mouse monoclonal antibody, clone OTI2D5 (formerly 2D5). Catalog# M11888. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Monkey.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-ACY3 Mouse Monoclonal Antibody [Clone ID: OTI2D5] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M11888)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI2D5
Isotype
IgG2b
Immunogen
Full length human recombinant protein of human ACY3 (NP_542389) produced in HEK293T cell.
Reactive Species
M11888 is reactive to ACY3 in Human, Monkey
Calculated molecular weight
35.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M11888 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500~2000
IHC 1:50
IF 1:100
Flow Cytometry 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACY3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACY3.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ACY3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ACY3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either ACY3 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACY3 antibody (M11888)
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Anti-ACY3 mouse monoclonal antibody (M11888) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACY3.
Specific Publications For Anti-ACY3 Mouse Monoclonal Antibody [Clone ID: OTI2D5] (M11888)
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