Western blot analysis of SMURF2 using anti-SMURF2 antibody (A02585-1).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HCT116 whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were traSMURF2erred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMURF2 antigen affinity purified polyclonal antibody (A02585-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SMURF2 at approximately 86 kDa. The expected band size for SMURF2 is at 86 kDa.