Anti-SMAD2 Antibody

Smad2 antibody

Boster Bio Anti-SMAD2 Antibody catalog # A00090-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 3 publication(s).

Product Info Summary

SKU: A00090-1
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-SMAD2 Antibody

View all Smad2 Antibodies

SKU/Catalog Number

A00090-1

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SMAD2 Antibody catalog # A00090-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SMAD2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00090-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human SMAD2 recombinant protein (Position: E83-Q264).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00090-1 is reactive to SMAD2 in Human, Mouse, Rat

Applications

A00090-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Background of Smad2

Smad2 (Mothers against decapentaplegic homolog 2), also known as MADR2, MADH2, SMAD family member 2 or SMAD2, is a protein that in humans is encoded by the SMAD2 gene. MAD homolog 2 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. Eppert et al. mapped the MADR2 gene close to DPC4 at 18q21, a region which is frequently deleted in colorectal cancers. Riggins et al. mapped the human MADH2 gene to 18q21. Nakao et al. refined the localization of the SMAD2 gene to 18q21.1, approximately 3 Mb proximal to DPC4, by fluorescence in situ hybridization. SMAD2 mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 5μg/ml
Flow Cytometry, 1-3μg/1x106 cells
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For SMAD2 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q15796

Gene ID

4087

Gene Name

SMAD2

Full Name

Mothers against decapentaplegic homolog 2

Weight

52.306kDa

Superfamily

dwarfin/SMAD family

Alternative Names

hMAD-2; hSMAD2; JV18; JV18-1SMAD, mothers against DPP homolog 2 (Drosophila); MAD homolog 2; Mad protein homolog; Mad, mothers against decapentaplegic homolog 2; MADH2MGC34440; MADR2MGC22139; Mad-related protein 2; mother against DPP homolog 2; mothers against decapentaplegic homolog 2; Mothers against DPP homolog 2; Sma- and Mad-related protein 2; SMAD 2; SMAD family member 2MAD, mothers against decapentaplegic homolog 2 (Drosophila); SMAD, mothers against DPP homolog 2; Smad2; Smad2/3 SMAD2 JV18, JV18-1, MADH2, MADR2, hMAD-2, hSMAD2 SMAD family member 2 mothers against decapentaplegic homolog 2|MAD homolog 2|SMAD, mothers against DPP homolog 2|Sma- and Mad-related protein 2|mother against DPP homolog 2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on SMAD2, check out the SMAD2 Infographic

SMAD2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for SMAD2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A00090-1 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Effect of Kuijie Granule on the Expression of TGF-β/Smads Signaling Pathway in Patients with Ulcerative Colitis

Mouse hepatic neoplasm formation induced by trace level and low frequency exposure to diethylnitrosamine through %u03B2-catenin signaling pathway

Astragaloside effect on TGF-%u03B21, SMAD2/3, and %u03B1-SMA expression in the kidney tissues of diabetic KKAy mice

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16 Customer Q&As for Anti-SMAD2 Antibody

Question

My question regards to test anti-SMAD2 antibody A00090-1 on mouse kidney for research purposes, then I may be interested in using anti-SMAD2 antibody A00090-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-04-29

Answer

The products we sell, including anti-SMAD2 antibody A00090-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-04-29

Question

I see that the anti-SMAD2 antibody A00090-1 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-11-28

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-11-28

Question

We ordered your anti-SMAD2 antibody for IHC-P on placenta a few years ago. I am using human, and I plan to use the antibody for WB next. I am interested in examining placenta as well as colon adenocarcinoma in our next experiment. Do you have any suggestion on which antibody would work the best for WB?

L. Taylor

Verified customer

Asked: 2019-10-29

Answer

I have checked the website and datasheets of our anti-SMAD2 antibody and it appears that A00090-1 has been validated on human in both IHC-P and WB. Thus A00090-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-10-29

Question

Would A00090-1 anti-SMAD2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-05-29

Answer

It shows on the product datasheet, A00090-1 anti-SMAD2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-05-29

Question

We were happy with the WB result of your anti-SMAD2 antibody. However we have seen positive staining in placenta cytoplasm using this antibody. Is that expected? Could you tell me where is SMAD2 supposed to be expressed?

A. Johnson

Verified customer

Asked: 2019-02-15

Answer

According to literature, placenta does express SMAD2. Generally SMAD2 expresses in cytoplasm. Regarding which tissues have SMAD2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Chronic myeloid leukemia cell, Pubmed ID: 8980228
Colon adenocarcinoma, Pubmed ID: 9702198
Kidney, Pubmed ID: 8752209
Kidney, Pancreas, and Spleen, Pubmed ID: 15489334
Placenta, Pubmed ID: 8774881

Boster Scientific Support

Answered: 2019-02-15

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-SMAD2 antibody A00090-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-01-03

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-01-03

Question

Do you have a BSA free version of anti-SMAD2 antibody A00090-1 available?

Verified Customer

Verified customer

Asked: 2018-07-06

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-SMAD2 antibody A00090-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-07-06

Question

We are currently using anti-SMAD2 antibody A00090-1 for rat tissue, and we are content with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?

W. Parker

Verified customer

Asked: 2017-12-15

Answer

The anti-SMAD2 antibody (A00090-1) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-12-15

Question

I have attached the WB image, lot number and protocol we used for kidney using anti-SMAD2 antibody A00090-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-11-13

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-11-13

Question

Is a blocking peptide available for product anti-SMAD2 antibody (A00090-1)?

Verified Customer

Verified customer

Asked: 2017-07-24

Answer

We do provide the blocking peptide for product anti-SMAD2 antibody (A00090-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-07-24

Question

I am interested in using your anti-SMAD2 antibody for smad4 mh2 domain mutants in cancer studies. Has this antibody been tested with western blotting on mouse heart tissue? We would like to see some validation images before ordering.

A. Jackson

Verified customer

Asked: 2016-09-09

Answer

We appreciate your inquiry. This A00090-1 anti-SMAD2 antibody is validated on human placenta tissue, rat liver tissue, mouse heart tissue, lung tissue. It is guaranteed to work for ELISA, IHC-P, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2016-09-09

Question

Is this A00090-1 anti-SMAD2 antibody reactive to the isotypes of SMAD2?

G. Li

Verified customer

Asked: 2015-05-13

Answer

The immunogen of A00090-1 anti-SMAD2 antibody is E.coli-derived human SMAD2 recombinant protein (Position: E83-Q264). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2015-05-13

Question

We have been able to see staining in mouse cervix carcinoma erythroleukemia. Do you have any suggestions? Is anti-SMAD2 antibody supposed to stain cervix carcinoma erythroleukemia positively?

S. Mangal

Verified customer

Asked: 2014-09-19

Answer

From what I have seen in literature cervix carcinoma erythroleukemia does express SMAD2. From what I have seen in Uniprot.org, SMAD2 is expressed in kidney, placenta, kidney, pancreas spleen, chronic myeloid leukemia cell, colon adenocarcinoma, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have SMAD2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Chronic myeloid leukemia cell, Pubmed ID: 8980228
Colon adenocarcinoma, Pubmed ID: 9702198
Kidney, Pubmed ID: 8752209
Kidney, Pancreas, and Spleen, Pubmed ID: 15489334
Placenta, Pubmed ID: 8774881

Boster Scientific Support

Answered: 2014-09-19

Question

I was wanting to use your anti-SMAD2 antibody for WB for mouse kidney on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse kidney identification?

N. Jackson

Verified customer

Asked: 2013-08-29

Answer

As indicated on the product datasheet, A00090-1 anti-SMAD2 antibody has been tested for ELISA, IHC-P, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse kidney in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-08-29

Question

My question regarding product A00090-1, anti-SMAD2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

E. Baker

Verified customer

Asked: 2013-05-06

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00090-1 anti-SMAD2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2013-05-06

Question

Would anti-SMAD2 antibody A00090-1 work for WB with kidney?

T. Miller

Verified customer

Asked: 2013-01-30

Answer

According to the expression profile of kidney, SMAD2 is highly expressed in kidney. So, it is likely that anti-SMAD2 antibody A00090-1 will work for WB with kidney.

Boster Scientific Support

Answered: 2013-01-30

Conjugation

how to order through PO

Size

Total: $315

Size:100μg

SKU:A00090-1

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A00090-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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