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- Table of Contents
ADAM10 antibody (A00566-2) performs well in ELISA, IF, IHC-P, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunofluorescence Validation of ADAM10 in MOLT4 Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed MOLT4 cells labeling ADAM10 with A00566-2 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunofluorescent analysis of 4% paraformaldehyde-fixed MOLT4 cells labeling ADAM10 with A00566-2 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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Anti-ADAM10 Antibody
ADAM10 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IF, IHC-P, ICC, WB |
Recommend Dilution
Application
Dilution
Species
WB: 1-2 μg/mL
IHC-P: 1-2 μg/mL
IF: 10-20 μg/mL
ICC/IF: 10-20 μg/mL.
Antibody validated: Western Blot in human
mouse and rat samples; Immunofluorescence in human
mouse and rat samples; Immunohistochemistry in human and mouse samples. All other applications and species not yet tested.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-ADAM10 Antibody
View all ADAM10 Antibodies
View all ADAM10 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
ADAM10 antibody can be stored at 4°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Host
Rabbit
Contents
ADAM10 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-ADAM10 antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human ADAM10. The immunogen is located within the last 50 amino acids of ADAM10.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
84.1 kDa
Background of ADAM10
Proinflammatory cytokine tumor necrosis factor-alpha (TNF-α) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-α, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-α, notch and its ligand delta. The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively. ADAM10 mRNA is expressed in a variety of human and bovine tissues.
Cite This Product
Anti-ADAM10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00566-2)
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Product Promise
We promise Anti-ADAM10 Antibody performs excellently in relevant applications for ELISA, IF, IHC-P, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A00566-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 6, 2026