Anti-ADAM10 Antibody Picoband™

ADAM10 antibody

Boster Bio Anti-ADAM10 Antibody Picoband™ catalog # A00566-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: A00566-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-ADAM10 Antibody Picoband™

View all ADAM10 Antibodies

SKU/Catalog Number

A00566-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ADAM10 Antibody Picoband™ catalog # A00566-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ADAM10 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00566-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human ADAM10 recombinant protein (Position: T214-D325).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00566-1 is reactive to ADAM10 in Human, Mouse, Rat

Applications

A00566-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

84.142kDa

Background of ADAM10

ADAM10, A Disintegrin and Metalloproteinase Domain 10, is also known as AD10. ADAM10 is a member of the ADAM family and members of this family are cell surface proteins with a unique structure possessing both potential adhesion and protease function. The ADAM10 gene is mapped to chromosome 15q21.3-q23. ADAM proteins contain an N-terminal signal sequence, followed by a prodomain, a metalloprotease-like domain, a disintegrin-like domain, a cysteine-rich region, an EGF -like repeat, a transmembrane domain, and a C-terminal cytoplasmic tail. Conversion of the membrane-bound precursor to a secreted mature protein is mediated by a protease termed TNFA convertase. ADAM10 possesses TNFA convertase activity.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For ADAM10 (Source: Uniprot.org, NCBI)

Gene Name

ADAM10

Full Name

Disintegrin and metalloproteinase domain-containing protein 10

Weight

84.142kDa

Alternative Names

a disintegrin and metalloprotease domain 10; a disintegrin and metalloproteinase domain 10; AD10; ADAM 10; ADAM metallopeptidase domain 10; ADAM10; CD156c antigen; CD156c; CDw156; disintegrin and metalloproteinase domain-containing protein 10; EC 3.4.24; HsT18717; kuz; Kuzbanian protein homolog; Kuzbanian; MADM; MADMEC 3.4.24.81; Mammalian disintegrin-metalloprotease ADAM10 AD10, AD18, CD156c, CDw156, HsT18717, MADM, RAK, kuz ADAM metallopeptidase domain 10 disintegrin and metalloproteinase domain-containing protein 10|a disintegrin and metalloprotease domain 10|kuzbanian protein homolog|mammalian disintegrin-metalloprotease

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ADAM10, check out the ADAM10 Infographic

ADAM10 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ADAM10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A00566-1 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Gemcitabine inhibits immune escape of pancreatic cancer by down regulating the soluble ULBP2 protein

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16 Customer Q&As for Anti-ADAM10 Antibody Picoband™

Question

We are currently using anti-ADAM10 antibody A00566-1 for mouse tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2020-03-05

Answer

The anti-ADAM10 antibody (A00566-1) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-05

Question

We have observed staining in human liver. Do you have any suggestions? Is anti-ADAM10 antibody supposed to stain liver positively?

Verified Customer

Verified customer

Asked: 2019-12-12

Answer

From literature liver does express ADAM10. From Uniprot.org, ADAM10 is expressed in testis, placenta, platelet, liver, leukemic t-cell, cervix carcinoma, among other tissues. Regarding which tissues have ADAM10 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 19159218, 24275569
Placenta, Pubmed ID: 14702039
Platelet, Pubmed ID: 16263699

Boster Scientific Support

Answered: 2019-12-12

Question

We were satisfied with the WB result of your anti-ADAM10 antibody. However we have observed positive staining in placenta cell membrane using this antibody. Is that expected? Could you tell me where is ADAM10 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-11-29

Answer

From literature, placenta does express ADAM10. Generally ADAM10 expresses in cell membrane. Regarding which tissues have ADAM10 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 19159218, 24275569
Placenta, Pubmed ID: 14702039
Platelet, Pubmed ID: 16263699

Boster Scientific Support

Answered: 2019-11-29

Question

Is there a BSA free version of anti-ADAM10 antibody A00566-1 available?

Verified Customer

Verified customer

Asked: 2019-11-01

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-ADAM10 antibody A00566-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-11-01

Question

Will A00566-1 anti-ADAM10 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-09-12

Answer

It shows on the product datasheet, A00566-1 anti-ADAM10 antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-09-12

Question

I have attached the WB image, lot number and protocol we used for liver using anti-ADAM10 antibody A00566-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-09-04

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-04

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-ADAM10 antibody A00566-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-08-15

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-15

Question

Would anti-ADAM10 antibody A00566-1 work for ELISA with liver?

R. Thomas

Verified customer

Asked: 2019-07-09

Answer

According to the expression profile of liver, ADAM10 is highly expressed in liver. So, it is likely that anti-ADAM10 antibody A00566-1 will work for ELISA with liver.

Boster Scientific Support

Answered: 2019-07-09

Question

Is this A00566-1 anti-ADAM10 antibody reactive to the isotypes of ADAM10?

Verified Customer

Verified customer

Asked: 2019-03-07

Answer

The immunogen of A00566-1 anti-ADAM10 antibody is E. coli-derived human ADAM10 recombinant protein (Position: T214-D325). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-03-07

Question

Our lab want to know about using your anti-ADAM10 antibody for toxin transport studies. Has this antibody been tested with western blotting on rat stomach tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2018-07-05

Answer

We appreciate your inquiry. This A00566-1 anti-ADAM10 antibody is tested on rat stomach tissue, thymus tissue. It is guaranteed to work for ELISA, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-07-05

Question

I have a question about product A00566-1, anti-ADAM10 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-05-22

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00566-1 anti-ADAM10 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-05-22

Question

We need to test anti-ADAM10 antibody A00566-1 on rat liver for research purposes, then I may be interested in using anti-ADAM10 antibody A00566-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-04-24

Answer

The products we sell, including anti-ADAM10 antibody A00566-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-04-24

Question

Our lab used your anti-ADAM10 antibody for WB on placenta in the past. I am using mouse, and We want to use the antibody for ELISA next. I am interested in examining placenta as well as liver in our next experiment. Could give a recommendation on which antibody would work the best for ELISA?

Verified Customer

Verified customer

Asked: 2018-01-12

Answer

I looked at the website and datasheets of our anti-ADAM10 antibody and it appears that A00566-1 has been validated on mouse in both WB and ELISA. Thus A00566-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-01-12

Question

I see that the anti-ADAM10 antibody A00566-1 works with ELISA, what is the protocol used to produce the result images on the product page?

C. Jones

Verified customer

Asked: 2014-07-18

Answer

You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2014-07-18

Question

Is a blocking peptide available for product anti-ADAM10 antibody (A00566-1)?

E. Wu

Verified customer

Asked: 2014-05-14

Answer

We do provide the blocking peptide for product anti-ADAM10 antibody (A00566-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2014-05-14

Question

I was wanting to use your anti-ADAM10 antibody for ELISA for rat liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat liver identification?

E. Zhang

Verified customer

Asked: 2013-02-06

Answer

As indicated on the product datasheet, A00566-1 anti-ADAM10 antibody has been validated for ELISA, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat liver in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-02-06

Size

Conjugation

Total: $370

SKU:A00566-1

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A00566-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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