Human SMPD1 ELISA Kit PicoKine®

SMPD1 ELISA kit for Human

Human SMPD1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human SMPD1 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK2025
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin).

Product Name

Human SMPD1 ELISA Kit PicoKine®

View all SMPD1 ELISA kits

SKU/Catalog Number

EK2025

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human SMPD1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human SMPD1 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human SMPD1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2025)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: CHO; Immunogen sequence: L47-C631

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2025 is reactive to SMPD1 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin).

Application Guarantee

EK2025 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of SMPD1

Sphingomyelin phosphodiesterase 1 (SMPD1), also known as acid sphingomyelinase (ASM), is an enzyme that in humans is encoded by the SMPD1 gene. This gene is mapped to 11p15.4. The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. Sphingomyelin phosphodiesterase 1 belongs to the sphingomyelin phosphodiesterase family.

Kit Components

Catalog Number Description Quantity
EK2025-CAP Anti-Human SMPD1 Pre-coated 96-well strip microplate 1
EK2025-ST Human SMPD1 Standard 2 vials, 10 ng/tube
EK2025-DA Human SMPD1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0320.0740.1400.2300.3690.6361.0581.702

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human SMPD1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3301190417335112154524
Standard deviation14.1961.88191.9621.4171.69334.78
CV (%)4.3%5.2%4.6%6.1%5.9%7.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of SMPD1 in ELISA kits from four different production batches/lots.

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 133033230830531915.34.8%
Sample 21190118512011218119943.150.9%
Sample 341734545462247964534231.235.1%
*number of samples for each test n=16.

Gene/Protein Information For SMPD1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P17405

Gene ID

6609

Gene Name

SMPD1

Full Name

Sphingomyelin phosphodiesterase

Weight

69.936kDa

Superfamily

acid sphingomyelinase family

Alternative Names

Acid sphingomyelinase; aSMase; ASMsphingomyelin phosphodiesterase; EC 3.1.4.12; NPD; SMPD1; sphingomyelin phosphodiesterase 1, acid lysosomal SMPD1 ASM, ASMASE, NPD sphingomyelin phosphodiesterase 1 sphingomyelin phosphodiesterase|Niemann-Pick type A/B|acid sphingomyelinase|sphingomyelin phosphodiesterase 1, acid lysosomal

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on SMPD1, check out the SMPD1 Infographic

SMPD1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for SMPD1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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7 Customer Q&As for Human SMPD1 ELISA Kit PicoKine®

Question

Q: is it okay to use EDTA plasma as samples in Human SMPD1 Picokine® ELISA Kit (Catalog # EK2025)?

Z. Allen

Verified customer

Asked: 2020-12-23

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of SMPD1 causing degradation of its protein structure. SMPD1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the SMPD1 ELISA, treating samples with different anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-12-23

Question

Q: if the enzyme conjugated SMPD1 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the SMPD1 antigen?

Verified Customer

Verified customer

Asked: 2020-11-13

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-11-13

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2019-02-21

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-02-21

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification SMPD1 using the EK2025 Human SMPD1 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-12-30

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.

Boster Scientific Support

Answered: 2018-12-30

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

C. Moore

Verified customer

Asked: 2018-12-27

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-12-27

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2018-01-31

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-01-31

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2017-05-22

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2017-05-22

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