Anti-ADAM10/ADAM10 Antibody

ADAM10 antibody

Boster Bio Anti-ADAM10/ADAM10 Antibody catalog # PA1535. Tested in WB applications. This antibody reacts with Human. Cited in 1 publication(s).

Product Info Summary

SKU: PA1535
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

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Product Name

Anti-ADAM10/ADAM10 Antibody

View all ADAM10 Antibodies

SKU/Catalog Number

PA1535

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ADAM10/ADAM10 Antibody catalog # PA1535. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ADAM10/ADAM10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1535)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human ADAM10.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1535 is reactive to ADAM10 in Human

Applications

PA1535 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

84.142kDa

Background of ADAM10

ADAM10, A Disintegrin and Metalloproteinase Domain 10, is also know as AD10. ADAM10 is a member of the ADAM family and members of this family are cell surface proteins with a unique structure possessing both potential adhesion and protease function. The ADAM10 gene is mapped to chromosome 15q21.3-q23. ADAM proteins contain an N-terminal signal sequence, followed by a prodomain, a metalloprotease-like domain, a disintegrin-like domain, a cysteine-rich region, an EGF -like repeat, a transmembrane domain, and a C-terminal cytoplasmic tail. Conversion of the membrane-bound precursor to a secreted mature protein is mediated by a protease termed TNFA convertase. ADAM10 possesses TNFA convertase activity.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For ADAM10 (Source: Uniprot.org, NCBI)

Gene Name

ADAM10

Full Name

Disintegrin and metalloproteinase domain-containing protein 10

Weight

84.142kDa

Alternative Names

a disintegrin and metalloprotease domain 10; a disintegrin and metalloproteinase domain 10; AD10; ADAM 10; ADAM metallopeptidase domain 10; ADAM10; CD156c antigen; CD156c; CDw156; disintegrin and metalloproteinase domain-containing protein 10; EC 3.4.24; HsT18717; kuz; Kuzbanian protein homolog; Kuzbanian; MADM; MADMEC 3.4.24.81; Mammalian disintegrin-metalloprotease ADAM10 AD10, AD18, CD156c, CDw156, HsT18717, MADM, RAK, kuz ADAM metallopeptidase domain 10 disintegrin and metalloproteinase domain-containing protein 10|a disintegrin and metalloprotease domain 10|kuzbanian protein homolog|mammalian disintegrin-metalloprotease

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ADAM10, check out the ADAM10 Infographic

ADAM10 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ADAM10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1535 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Gemcitabine inhibits immune escape of pancreatic cancer by down regulating the soluble ULBP2 protein

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3 Customer Q&As for Anti-ADAM10/ADAM10 Antibody

Question

We have observed staining in human placenta. Are there any suggestions? Is anti-ADAM10/ADAM10 antibody supposed to stain placenta positively?

B. Mitchell

Verified customer

Asked: 2020-02-27

Answer

From what I have seen in literature placenta does express ADAM10. From what I have seen in Uniprot.org, ADAM10 is expressed in testis, placenta, platelet, liver, leukemic t-cell, cervix carcinoma, among other tissues. Regarding which tissues have ADAM10 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 19159218, 24275569
Placenta, Pubmed ID: 14702039
Platelet, Pubmed ID: 16263699

Boster Scientific Support

Answered: 2020-02-27

Question

We are currently using anti-ADAM10/ADAM10 antibody PA1535 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-11-29

Answer

The anti-ADAM10/ADAM10 antibody (PA1535) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-11-29

Question

Our team were content with the WB result of your anti-ADAM10/ADAM10 antibody. However we have seen positive staining in platelet cell membrane using this antibody. Is that expected? Could you tell me where is ADAM10 supposed to be expressed?

M. Parker

Verified customer

Asked: 2015-04-28

Answer

From what I have seen in literature, platelet does express ADAM10. Generally ADAM10 expresses in cell membrane. Regarding which tissues have ADAM10 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 19159218, 24275569
Placenta, Pubmed ID: 14702039
Platelet, Pubmed ID: 16263699

Boster Scientific Support

Answered: 2015-04-28

Size

Conjugation

Total: $370

SKU:PA1535

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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