Product Info Summary
| SKU: | PA1535 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-ADAM10/ADAM10 Antibody Picoband®
SKU/Catalog Number
PA1535
BA3490-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ADAM10/ADAM10 Antibody catalog # PA1535. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ADAM10/ADAM10 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1535)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human ADAM10.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1535 is reactive to ADAM10 in Human
Observed Molecular Weight
31 kDa
Calculated molecular weight
84.1 kDa
Background of ADAM10
ADAM10, A Disintegrin and Metalloproteinase Domain 10, is also know as AD10. ADAM10 is a member of the ADAM family and members of this family are cell surface proteins with a unique structure possessing both potential adhesion and protease function. The ADAM10 gene is mapped to chromosome 15q21.3-q23. ADAM proteins contain an N-terminal signal sequence, followed by a prodomain, a metalloprotease-like domain, a disintegrin-like domain, a cysteine-rich region, an EGF -like repeat, a transmembrane domain, and a C-terminal cytoplasmic tail. Conversion of the membrane-bound precursor to a secreted mature protein is mediated by a protease termed TNFA convertase. ADAM10 possesses TNFA convertase activity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1535 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Anti-ADAM10 antibody, PA1535, Western blotting
Lane 1: Recombinant Human ADAM10 Protein 10ng
Lane 2: Recombinant Human ADAM10 Protein 5ng
Lane 3: Recombinant Human ADAM10 Protein 2.5ng
Click image to see more details
Gemcitabine-mediated shedding of ULBP2 is ADAM10-dependent. A. ADAM10 expression of PANC-1 and MIA PACA-2 cells was determined when 2 μmol/l gemcitabine was added into cell culture. B. Cells were transfected with ADAM10 siRNA or control siRNA for 48 h and the expression of mRNA and protein of ADAM10 was evaluated by Q-PCR and western blot. C. sULBP2 in the culture supernatant was evaluated by ELISA. *P<0.05.
Index in PubMed under a CC BY license. PMID: 27602753
Click image to see more details
Immunohistochemical staining for ADAM10. The ADAM10 were principally localized in cytoplasm of tumor cells with varying staining intensity. A. High expression of ADAM10 (200x). B. High expression of ADAM10 (400x). C. Low expression of ADAM10 (200x). D. Low expression of ADAM10 (400x). E. Negative ADAM10 expression (200x). F. Partial enlargement of ADAM10 staining with the magnifying power of 400x.
Index in PubMed under a CC BY license. PMID: 27602753
Specific Publications For Anti-ADAM10/ADAM10 Antibody Picoband® (PA1535)
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Customer Q&As
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3 Customer Q&As for Anti-ADAM10/ADAM10 Antibody Picoband®
Question
We have observed staining in human placenta. Are there any suggestions? Is anti-ADAM10/ADAM10 antibody supposed to stain placenta positively?
B. Mitchell
Verified customer
Asked: 2020-02-27
Answer
From what I have seen in literature placenta does express ADAM10. From what I have seen in Uniprot.org, ADAM10 is expressed in testis, placenta, platelet, liver, leukemic t-cell, cervix carcinoma, among other tissues. Regarding which tissues have ADAM10 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 19159218, 24275569
Placenta, Pubmed ID: 14702039
Platelet, Pubmed ID: 16263699
Boster Scientific Support
Answered: 2020-02-27
Question
We are currently using anti-ADAM10/ADAM10 antibody PA1535 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
The anti-ADAM10/ADAM10 antibody (PA1535) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-29
Question
Our team were content with the WB result of your anti-ADAM10/ADAM10 antibody. However we have seen positive staining in platelet cell membrane using this antibody. Is that expected? Could you tell me where is ADAM10 supposed to be expressed?
M. Parker
Verified customer
Asked: 2015-04-28
Answer
From what I have seen in literature, platelet does express ADAM10. Generally ADAM10 expresses in cell membrane. Regarding which tissues have ADAM10 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 19159218, 24275569
Placenta, Pubmed ID: 14702039
Platelet, Pubmed ID: 16263699
Boster Scientific Support
Answered: 2015-04-28


