Anti-ADAMTS4/ADAMTS4 Antibody

Rabbit IgG polyclonal antibody for A disintegrin and metalloproteinase with thrombospondin motifs 4(ADAMTS4) detection. Tested with WB in Human;Mouse;Rat.

SKU PA1479
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications WB

Overview

Product Name Anti-ADAMTS4/ADAMTS4 Antibody
See all ADAMTS4 primary antibodies, ELISA kits and proteins
SKU/Catalog Number PA1479
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Polyclonal antibody for ADAMTS4 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. ADAMTS4 information: Molecular Weight: 90197 MW; Subcellular Localization: Secreted, extracellular space, extracellular matrix ; Tissue Specificity: Expressed in brain, lung and heart. Expressed at very low level in placenta and skeletal muscles. Isoform 2 is detected in osteoarthritic synovium.
Cite This Product Anti-ADAMTS4/ADAMTS4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1479)
Specificity Anti-ADAMTS4/ADAMTS4 Antibody (PA1479) reacts with Human, Mouse, Rat ADAMTS4, in native form and recombinant. Superfamily members of ADAMTS4 are not reactive to PA1479.
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Form Lyophilized
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human ADAMTS4(820-837aa RRAQILEILRRRPWAGRK), different from the related rat and mouse sequences by three amino acids.
Reactivity Human, Mouse, Rat

Assay Details

Our Boster Quality Guarantee for Anti-ADAMTS4/ADAMTS4 Antibody covers its use in the following applications.

*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Boster's Compatible Products

The following reagents are used to generate the images below for Anti-ADAMTS4/ADAMTS4 Antibody (PA1479).

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Images And Assay Conditions

/antibody/pa1479-1-WB-anti-adamts4-antibody.jpg

Anti-ADAMTS4 antibody, PA1479, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Mouse Brain Tissue Lysate

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id O75173
Gene Name ADAMTS4
Protein Name A disintegrin and metalloproteinase with thrombospondin motifs 4
Tissue Specificity Expressed in brain, lung and heart. Expressed at very low level in placenta and skeletal muscles. Isoform 2 is detected in osteoarthritic synovium. .
Alternative Names A disintegrin and metalloproteinase with thrombospondin motifs 4;ADAM-TS 4;ADAM-TS4;ADAMTS-4;3.4.24.82;ADMP-1;Aggrecanase-1;ADAMTS4;KIAA0688;UNQ769/PRO1563;
Subcellular Localization Secreted, extracellular space, extracellular matrix .
Molecular Weight 90197 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu-|-Ala-393' site.
Research Areas Human, Mouse, Rat

*You can search these to find other products in these research areas.
Background ADAMTS4, A disintegrin and metalloproteinase with thrombospondin motifs 4 is an enzyme that in humans is encoded by the ADAMTS4 gene. ADAMTS4 is a member of the large ADAMTS family of zinc-dependent proteases. The human ADAMTS4 gene is mapped to chromosome 1 by somatic cell hybrid analysis. The enzyme encoded by this gene lacks a C-terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma.

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PA1479
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

Troubleshooting

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Publications

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Customer Q&As

  • Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
    A: Yes, please contact us at [email protected] for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact [email protected]
  • Q: What should I use for negative control?
    A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
  • Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
    A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
  • Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
    A: You can find the immunogen sequence under "
  • Q: What is the expected band size? Why is it different than the observed band size?
    A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
  • Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
    A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
  • Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
    A: Check our protocols under the tech support tab.
  • Q: What are some alternative names that could be used to describe this product?
    A: Some common names include but are not limited to adamts2 antibody, adamts4 antibody
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