Anti-ADAMTS4/ADAMTS4 Antibody Picoband™

ADAMTS4 antibody

Boster Bio Anti-ADAMTS4/ADAMTS4 Antibody Picoband™ catalog # A02899. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. Cited in 1 publication(s).

Product Info Summary

SKU: A02899
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA, IHC, WB

Product Name

Anti-ADAMTS4/ADAMTS4 Antibody Picoband™

View all ADAMTS4 Antibodies

SKU/Catalog Number

A02899

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ADAMTS4/ADAMTS4 Antibody Picoband™ catalog # A02899. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ADAMTS4/ADAMTS4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02899)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human ADAMTS4, different from the related mouse sequence by five amino acids, and from the related rat sequence by six amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A02899 is reactive to ADAMTS4 in Human

Applications

A02899 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

58 kDa

Calculated molecular weight

90.197kDa

Background of ADAMTS4

ADAMTS4, A disintegrin and metalloproteinase with thrombospondin motifs 4, is an enzyme that in humans is encoded by the ADAMTS4 gene. ADAMTS4 is a member of the large ADAMTS family of zinc-dependent proteases. The human ADAMTS4 gene is mapped to chromosome 1 by somatic cell hybrid analysis. The enzyme encoded by this gene lacks a C-terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
ELISA , 0.1-0.5μg/ml, Human, -
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For ADAMTS4 (Source: Uniprot.org, NCBI)

Gene Name

ADAMTS4

Full Name

A disintegrin and metalloproteinase with thrombospondin motifs 4

Weight

90.197kDa

Alternative Names

a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 4; ADAM metallopeptidase with thrombospondin type 1 motif, 4; ADAM-TS 4; ADAMTS2; ADAMTS-2; ADAMTS4; ADAM-TS4; ADAMTS-4; ADMP-1; ADMP-1EC 3.4.24.82; Aggrecanase 1; aggrecanase-1; EC 3.4.24; KIAA0688A disintegrin and metalloproteinase with thrombospondin motifs 4 ADAMTS4 ADAMTS-2, ADAMTS-4, ADMP-1 ADAM metallopeptidase with thrombospondin type 1 motif 4 A disintegrin and metalloproteinase with thrombospondin motifs 4|ADAM-TS 4|ADAM-TS4|a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 4|aggrecanase-1|epididymis secretory sperm binding protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ADAMTS4, check out the ADAMTS4 Infographic

ADAMTS4 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ADAMTS4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A02899 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Electrospun Poly(L-lactide)/Poly(?-caprolactone) Blend Nanofibrous Scaffold: Characterization and Biocompatibility with Human Adipose-Derived Stem Cells

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7 Customer Q&As for Anti-ADAMTS4/ADAMTS4 Antibody Picoband™

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for left coronary artery using anti-ADAMTS4/ADAMTS4 antibody A02899. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-03-19

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-19

Question

Is a blocking peptide available for product anti-ADAMTS4/ADAMTS4 antibody (A02899)?

Verified Customer

Verified customer

Asked: 2020-03-06

Answer

We do provide the blocking peptide for product anti-ADAMTS4/ADAMTS4 antibody (A02899). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-03-06

Question

Can A02899 be used to detect swine samples?

Verified Customer

Verified customer

Asked: 2019-12-16

Answer

With A02899, swine samples have a 84% sequence homology identity with human.

Boster Scientific Support

Answered: 2019-12-16

Question

Is this A02899 anti-ADAMTS4/ADAMTS4 antibody reactive to the isotypes of ADAMTS4?

Verified Customer

Verified customer

Asked: 2019-02-14

Answer

The immunogen of A02899 anti-ADAMTS4/ADAMTS4 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human ADAMTS4 (813-837aa TPQDWLHRRAQILEILRRRPWAGRK), different from the related mouse sequence by five amino acids, and from the related rat sequence by six amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-02-14

Question

Will anti-ADAMTS4/ADAMTS4 antibody A02899 work for IHC with left coronary artery?

Verified Customer

Verified customer

Asked: 2017-12-13

Answer

According to the expression profile of left coronary artery, ADAMTS4 is highly expressed in left coronary artery. So, it is likely that anti-ADAMTS4/ADAMTS4 antibody A02899 will work for IHC with left coronary artery.

Boster Scientific Support

Answered: 2017-12-13

Question

I have a question about product A02899, anti-ADAMTS4/ADAMTS4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2017-12-04

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02899 anti-ADAMTS4/ADAMTS4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-12-04

Question

Would A02899 anti-ADAMTS4/ADAMTS4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-10-20

Answer

You can see on the product datasheet, A02899 anti-ADAMTS4/ADAMTS4 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-10-20

Size

Conjugation

Total: $370

SKU:A02899

Backordered.

Lead time for this item is typically 2-3 weeks

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A02899
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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