Click image to see more details
Product Info Summary
| SKU: | A02899 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-ADAMTS4/ADAMTS4 Antibody Picoband®
SKU/Catalog Number
A02899
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ADAMTS4/ADAMTS4 Antibody Picoband® catalog # A02899. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ADAMTS4/ADAMTS4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02899)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human ADAMTS4, different from the related mouse sequence by five amino acids, and from the related rat sequence by six amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A02899 is reactive to ADAMTS4 in Human
Observed Molecular Weight
58 kDa
Calculated molecular weight
90.2 kDa
Background of ADAMTS4
ADAMTS4, A disintegrin and metalloproteinase with thrombospondin motifs 4, is an enzyme that in humans is encoded by the ADAMTS4 gene. ADAMTS4 is a member of the large ADAMTS family of zinc-dependent proteases. The human ADAMTS4 gene is mapped to chromosome 1 by somatic cell hybrid analysis. The enzyme encoded by this gene lacks a C-terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02899 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
ELISA, 0.1-0.5μg/ml, -
Validation Images & Assay Conditions
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Western blot analysis of ADAMTS4 using anti-ADAMTS4 antibody (A02899).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Recombinant Human ADAMTS4 Protein 0.5ng
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAMTS4 antigen affinity purified polyclonal antibody (Catalog # A02899) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAMTS4 at approximately 58KD. The expected band size for ADAMTS4 is at 53KD.
Specific Publications For Anti-ADAMTS4/ADAMTS4 Antibody Picoband® (A02899)
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Customer Q&As
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7 Customer Q&As for Anti-ADAMTS4/ADAMTS4 Antibody Picoband®
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for left coronary artery using anti-ADAMTS4/ADAMTS4 antibody A02899. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-19
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-19
Question
Is a blocking peptide available for product anti-ADAMTS4/ADAMTS4 antibody (A02899)?
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
We do provide the blocking peptide for product anti-ADAMTS4/ADAMTS4 antibody (A02899). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-03-06
Question
Can A02899 be used to detect swine samples?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
With A02899, swine samples have a 84% sequence homology identity with human.
Boster Scientific Support
Answered: 2019-12-16
Question
Is this A02899 anti-ADAMTS4/ADAMTS4 antibody reactive to the isotypes of ADAMTS4?
Verified Customer
Verified customer
Asked: 2019-02-14
Answer
The immunogen of A02899 anti-ADAMTS4/ADAMTS4 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human ADAMTS4 (813-837aa TPQDWLHRRAQILEILRRRPWAGRK), different from the related mouse sequence by five amino acids, and from the related rat sequence by six amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-02-14
Question
Will anti-ADAMTS4/ADAMTS4 antibody A02899 work for IHC with left coronary artery?
Verified Customer
Verified customer
Asked: 2017-12-13
Answer
According to the expression profile of left coronary artery, ADAMTS4 is highly expressed in left coronary artery. So, it is likely that anti-ADAMTS4/ADAMTS4 antibody A02899 will work for IHC with left coronary artery.
Boster Scientific Support
Answered: 2017-12-13
Question
I have a question about product A02899, anti-ADAMTS4/ADAMTS4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-12-04
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02899 anti-ADAMTS4/ADAMTS4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-12-04
Question
Would A02899 anti-ADAMTS4/ADAMTS4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-10-20
Answer
You can see on the product datasheet, A02899 anti-ADAMTS4/ADAMTS4 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-10-20
