Anti-ADAR1 Monoclonal Antibody

ADAR antibody

Boster Bio Anti-ADAR1 Monoclonal Antibody catalog # M00869. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.

Product Info Summary

SKU: M00869
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-ADAR1 Monoclonal Antibody

View all ADAR Antibodies

SKU/Catalog Number

M00869

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-ADAR1 Monoclonal Antibody catalog # M00869. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ADAR1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00869)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AFCB-1

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human ADAR1 Converts multiple adenosines to inosines and creates I/U mismatched base pairs in double-helical RNA substrates without apparent sequence specificity.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00869 is reactive to ADAR in Human

Applications

M00869 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

150 kDa

Calculated molecular weight

136.066kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For ADAR (Source: Uniprot.org, NCBI)

Gene Name

ADAR

Full Name

Double-stranded RNA-specific adenosine deaminase

Weight

136.066kDa

Alternative Names

ADAR1DSH; adenosine deaminase, RNA-specific; DRADAP136; DSRADadenosine deaminase acting on RNA 1-A; EC 3.5.4; EC 3.5.4.-; G1P1double-stranded RNA-specific adenosine deaminase; IFI-4; IFI4dsRNA adenosine deaminase; interferon-induced protein 4; Interferon-inducible protein 4; K88DSRBP; p136,136 kDa double-stranded RNA-binding protein ADAR ADAR1, AGS6, DRADA, DSH, DSRAD, G1P1, IFI-4, IFI4, K88DSRBP, P136 adenosine deaminase RNA specific double-stranded RNA-specific adenosine deaminase|136 kDa double-stranded RNA-binding protein|adenosine deaminase acting on RNA 1-A|dsRNA adenosine deaminase|dsRNA adeonosine deaminase|interferon-induced protein 4|interferon-inducible protein 4

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ADAR, check out the ADAR Infographic

ADAR infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ADAR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-ADAR1 Monoclonal Antibody

Question

Is a blocking peptide available for product anti-ADAR1 Monoclonal antibody (M00869)?

Verified Customer

Verified customer

Asked: 2019-07-01

Answer

We do provide the blocking peptide for product anti-ADAR1 Monoclonal antibody (M00869). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-07-01

Question

Would M00869 anti-ADAR1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-09-29

Answer

It shows on the product datasheet, M00869 anti-ADAR1 Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-09-29

Question

Can you help my question with product M00869, anti-ADAR1 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

P. Wu

Verified customer

Asked: 2017-09-05

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00869 anti-ADAR1 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-09-05

Question

Would anti-ADAR1 Monoclonal antibody M00869 work on zebrafish WB with lymph?

B. Rodriguez

Verified customer

Asked: 2015-09-10

Answer

Our lab technicians have not validated anti-ADAR1 Monoclonal antibody M00869 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-ADAR1 Monoclonal antibody M00869 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish lymph in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-09-10

Size

Total: $370

SKU:M00869

Backordered.

Lead time for this item is typically 3-4 weeks

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Eddy test
In stock
Order Product
M00869
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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