Product Info Summary
SKU: | A05238 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Adenosine receptor A2b ADORA2B Antibody
View all Adenosine A2b R Antibodies
SKU/Catalog Number
A05238
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Adenosine receptor A2b ADORA2B Antibody (Catalog # A05238). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ADORA2B antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Adenosine receptor A2b ADORA2B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05238)
Host
Rabbit
Contents
ADORA2B antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ADORA2B antibody was raised against a 19 amino acid peptide near the carboxy terminus of human ADORA2B. The immunogen is located within the last 50 amino acids of ADORA2B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
ADORA2B antibody is human, mouse and rat reactive. At least two isoforms of ADORA2B are known to exist; this antibody will detect both isoforms. ADORA2B antibody is predicted to not cross-react with ADORA2A.
Reactive Species
A05238 is reactive to ADORA2B in Human, Mouse, Rat
Applications
A05238 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
36.333kDa
Background of Adenosine A2b R
The adenosine receptor ADORA2B is a member of the G protein-coupled receptor superfamily and is an integral membrane protein that stimulates adenylate cyclase activity in the presence of adenosine (1,2). Extracellular adenosine triggers a potent anti-inflammatory response that is mediated in part by ADORA2B including the stimulation of IL-10 production (3-5). Activation of ADORA2B can also enhance the abundance of regulatory T cells (Tregs), a class of cells that work to constrain inflammation (6).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ADORA2B antibody can be used for detection of ADORA2B by Western blot at 0.25 – 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ADORA2B in mouse kidney tissue lysate with ADORA2B antibody at (A) 0.25 and (B) 0.5 μg/mL
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Immunohistochemistry of ADORA2B in rat colon tissue with ADORA2B antibody at 5 μg/mL.
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Immunofluorescence of ADORA2B in rat colon tissue with ADORA2B antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For ADORA2B (Source: Uniprot.org, NCBI)
Gene Name
ADORA2B
Full Name
Adenosine receptor A2b
Weight
36.333kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
Adenosine A2b R; adenosine A2b receptor; Adenosine A2bR; adenosine receptor A2b; ADORA2; ADORA2B ADORA2B ADORA2 adenosine A2b receptor adenosine receptor A2b
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ADORA2B, check out the ADORA2B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ADORA2B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Adenosine receptor A2b ADORA2B Antibody (A05238)
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