Anti-Adenosine receptor A2b ADORA2B Antibody

Product Name

Anti-Adenosine receptor A2b ADORA2B Antibody

View all ADORA2B Antibodies

SKU/Catalog Number

A05238

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Adenosine receptor A2b ADORA2B Antibody (Catalog # A05238). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

ADORA2B antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-Adenosine receptor A2b ADORA2B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05238)

Host

Rabbit

Contents

ADORA2B antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

ADORA2B antibody was raised against a 19 amino acid peptide near the carboxy terminus of human ADORA2B. The immunogen is located within the last 50 amino acids of ADORA2B.

Cross-reactivity

ADORA2B antibody is human, mouse and rat reactive. At least two isoforms of ADORA2B are known to exist; this antibody will detect both isoforms. ADORA2B antibody is predicted to not cross-react with ADORA2A.

Reactive Species

A05238 is reactive to ADORA2B in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

36.3 kDa

Background of ADORA2B

The adenosine receptor ADORA2B is a member of the G protein-coupled receptor superfamily and is an integral membrane protein that stimulates adenylate cyclase activity in the presence of adenosine (1,2). Extracellular adenosine triggers a potent anti-inflammatory response that is mediated in part by ADORA2B including the stimulation of IL-10 production (3-5). Activation of ADORA2B can also enhance the abundance of regulatory T cells (Tregs), a class of cells that work to constrain inflammation (6).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A05238 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ADORA2B antibody can be used for detection of ADORA2B by Western blot at 0.25 – 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information ADORA2B

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