Anti-Alcohol Dehydrogenase/ADH1A Antibody Picoband™

Boster Bio Anti-Alcohol Dehydrogenase/ADH1A Antibody Picoband™ catalog # PB10029. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PB10029
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC-P, ICC, WB

Product Name

Anti-Alcohol Dehydrogenase/ADH1A Antibody Picoband™

See all ADH1A products

SKU/Catalog Number

PB10029

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Alcohol Dehydrogenase/ADH1A Antibody Picoband™ catalog # PB10029. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Alcohol Dehydrogenase/ADH1A Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10029)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived human ADH1A recombinant protein (Position: K213-F375). Human ADH1A shares 87.1% and 82.8% amino acid (aa) sequence identity with mouse and rat ADH1A, respectively.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PB10029 is reactive to ADH1A in Human, Mouse, Rat

Applications

PB10029 is guaranteed for Flow Cytometry, IF, IHC-P, ICC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For ADH1A (Source: Uniprot.Org, NCBI)

Uniprot ID

P07327

Gene ID

124

Gene Name

ADH1A

Full Name

Alcohol dehydrogenase 1A

Weight

39.859kDa

Superfamily

zinc-containing alcohol dehydrogenase family

Alternative Names

ADH, alpha subunit; ADH1alcohol dehydrogenase 1A; alcohol dehydrogenase 1 (class I), alpha polypeptide; alcohol dehydrogenase 1A (class I), alpha polypeptide; Alcohol dehydrogenase subunit alpha; aldehyde reductase; EC 1.1.1; EC 1.1.1.1 ADH1A ADH1 alcohol dehydrogenase 1A (class I), alpha polypeptide alcohol dehydrogenase 1A|ADH, alpha subunit|alcohol dehydrogenase 1 (class I), alpha polypeptide|alcohol dehydrogenase subunit alpha|aldehyde reductase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ADH1A, check out the ADH1A Infographic

ADH1A infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ADH1A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for PB10029

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-Alcohol Dehydrogenase/ADH1A Antibody Picoband™?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-Alcohol Dehydrogenase/ADH1A Antibody Picoband™

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

7 Customer Q&As for Anti-Alcohol Dehydrogenase/ADH1A Antibody Picoband™

Question

I was wanting to use your anti-Alcohol Dehydrogenase/ADH1A antibody for IHC for rat brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat brain identification?

Verified Customer

Verified customer

Asked: 2020-01-22

Answer

As indicated on the product datasheet, PB10029 anti-Alcohol Dehydrogenase/ADH1A antibody has been validated for Flow Cytometry, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-01-22

Question

Do you have a BSA free version of anti-Alcohol Dehydrogenase/ADH1A antibody PB10029 available?

Verified Customer

Verified customer

Asked: 2019-12-09

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Alcohol Dehydrogenase/ADH1A antibody PB10029 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-12-09

Question

We are currently using anti-Alcohol Dehydrogenase/ADH1A antibody PB10029 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2019-10-01

Answer

The anti-Alcohol Dehydrogenase/ADH1A antibody (PB10029) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-10-01

Question

Does anti-Alcohol Dehydrogenase/ADH1A antibody PB10029 work on bovine WB with liver?

Verified Customer

Verified customer

Asked: 2019-08-27

Answer

Our lab technicians have not validated anti-Alcohol Dehydrogenase/ADH1A antibody PB10029 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-Alcohol Dehydrogenase/ADH1A antibody PB10029 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine liver in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-27

Question

Is a blocking peptide available for product anti-Alcohol Dehydrogenase/ADH1A antibody (PB10029)?

Verified Customer

Verified customer

Asked: 2018-11-07

Answer

We do provide the blocking peptide for product anti-Alcohol Dehydrogenase/ADH1A antibody (PB10029). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-11-07

Question

Does PB10029 anti-Alcohol Dehydrogenase/ADH1A antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

P. Patel

Verified customer

Asked: 2016-11-10

Answer

As indicated on the product datasheet, PB10029 anti-Alcohol Dehydrogenase/ADH1A antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2016-11-10

Question

My question regarding product PB10029, anti-Alcohol Dehydrogenase/ADH1A antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

C. Moore

Verified customer

Asked: 2014-09-30

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB10029 anti-Alcohol Dehydrogenase/ADH1A antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2014-09-30

Conjugation

Size

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product
PB10029
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.