Product Info Summary
SKU: | PB10029 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Alcohol Dehydrogenase/ADH1A Antibody Picoband™
SKU/Catalog Number
PB10029
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Alcohol Dehydrogenase/ADH1A Antibody Picoband™ catalog # PB10029. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Alcohol Dehydrogenase/ADH1A Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10029)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human ADH1A recombinant protein (Position: K213-F375). Human ADH1A shares 87.1% and 82.8% amino acid (aa) sequence identity with mouse and rat ADH1A, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB10029 is reactive to ADH1A in Human, Mouse, Rat
Applications
PB10029 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
40 kDa
Calculated molecular weight
39.859kDa
Background of ADH1A
Alcohol dehydrogenase 1A is an enzyme that in humans is encoded by the ADH1A gene. This gene encodes a member of the alcohol dehydrogenase family. The encoded protein is the alpha subunit of class I alcohol dehydrogenase, which consists of several homo- and heterodimers of alpha, beta and gamma subunits. Alcohol dehydrogenases catalyze the oxidation of alcohols to aldehydes. This gene is active in the liver in early fetal life but only weakly active in adult liver. And this gene is found in a cluster with six additional alcohol dehydrogenase genes, including those encoding the beta and gamma subunits, on the long arm of chromosome 4. Mutations in this gene may contribute to variation in certain personality traits and substance dependence.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ADH1A using anti-ADH1A antibody (PB10029).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HCCT tissue lysates,
Lane 2: human HCCP tissue lysates,
Lane 3: rat liver tissue lysates.
Lane 4: rat kidney tissue lysates.
Lane 5: mouse liver tissue lysates.
Lane 6: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADH1A antigen affinity purified polyclonal antibody (Catalog # PB10029) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADH1A at approximately 40 kDa. The expected band size for ADH1A is at 40 kDa.
Click image to see more details
Figure 2. IHC analysis of ADH1A using anti-ADH1A antibody (PB10029).
ADH1A was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ADH1A Antibody (PB10029) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of ADH1A using anti-ADH1A antibody (PB10029).
ADH1A was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ADH1A Antibody (PB10029) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of ADH1A using anti-ADH1A antibody (PB10029).
ADH1A was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ADH1A Antibody (PB10029) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 5. IF analysis of ADH1A using anti-ADH1A antibody (PB10029).
ADH1A was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ADH1A Antibody (PB10029) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 6. Flow Cytometry analysis of U20S cells using anti-ADH1A antibody (PB10029).
Overlay histogram showing U20S cells stained with PB10029 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ADH1A Antibody (PB10029, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For ADH1A (Source: Uniprot.org, NCBI)
Gene Name
ADH1A
Full Name
Alcohol dehydrogenase 1A
Weight
39.859kDa
Superfamily
zinc-containing alcohol dehydrogenase family
Alternative Names
ADH, alpha subunit; ADH1alcohol dehydrogenase 1A; alcohol dehydrogenase 1 (class I), alpha polypeptide; alcohol dehydrogenase 1A (class I), alpha polypeptide; Alcohol dehydrogenase subunit alpha; aldehyde reductase; EC 1.1.1; EC 1.1.1.1 ADH1A ADH1 alcohol dehydrogenase 1A (class I), alpha polypeptide alcohol dehydrogenase 1A|ADH, alpha subunit|alcohol dehydrogenase 1 (class I), alpha polypeptide|alcohol dehydrogenase subunit alpha|aldehyde reductase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ADH1A, check out the ADH1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ADH1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Alcohol Dehydrogenase/ADH1A Antibody Picoband™ (PB10029)
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No publications found for PB10029
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Alcohol Dehydrogenase/ADH1A Antibody Picoband™
Question
I was wanting to use your anti-Alcohol Dehydrogenase/ADH1A antibody for IHC for rat brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat brain identification?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
As indicated on the product datasheet, PB10029 anti-Alcohol Dehydrogenase/ADH1A antibody has been validated for Flow Cytometry, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-22
Question
Do you have a BSA free version of anti-Alcohol Dehydrogenase/ADH1A antibody PB10029 available?
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Alcohol Dehydrogenase/ADH1A antibody PB10029 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-09
Question
We are currently using anti-Alcohol Dehydrogenase/ADH1A antibody PB10029 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-01
Answer
The anti-Alcohol Dehydrogenase/ADH1A antibody (PB10029) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-01
Question
Does anti-Alcohol Dehydrogenase/ADH1A antibody PB10029 work on bovine WB with liver?
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
Our lab technicians have not validated anti-Alcohol Dehydrogenase/ADH1A antibody PB10029 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-Alcohol Dehydrogenase/ADH1A antibody PB10029 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-27
Question
Is a blocking peptide available for product anti-Alcohol Dehydrogenase/ADH1A antibody (PB10029)?
Verified Customer
Verified customer
Asked: 2018-11-07
Answer
We do provide the blocking peptide for product anti-Alcohol Dehydrogenase/ADH1A antibody (PB10029). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-11-07
Question
Does PB10029 anti-Alcohol Dehydrogenase/ADH1A antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
P. Patel
Verified customer
Asked: 2016-11-10
Answer
As indicated on the product datasheet, PB10029 anti-Alcohol Dehydrogenase/ADH1A antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-11-10
Question
My question regarding product PB10029, anti-Alcohol Dehydrogenase/ADH1A antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
C. Moore
Verified customer
Asked: 2014-09-30
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB10029 anti-Alcohol Dehydrogenase/ADH1A antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-09-30