Anti-ADIPOR1 Rabbit Monoclonal Antibody

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human kidney, using ADIPOR1 Antibody.

Mechanisms for local medulla injection of ADPN promoting bone healing. (A) Serum Osteoprotegerin (OPG) levels at weeks 2, 4, and 6. (B) WB analysis of alkaline phosphatase (ALP), bone morphogenic protein 2 (BMP-2), osteocalcin (OCN), and adiponectin receptor 1 (AdipoR1) expressions with corresponding quantification, (C) Immunofluorescent staining of ALP, OCN, BMP-2, and AdipoR1 in G2 (ADPN 1 mg/kg) and G3 (ADPN 2 mg/kg), and the corresponding quantification, n = 5. The white arrow indicates the periosteum, and the blue arrow indicates the lacuna, scale bar = 100 μm.
Index in PubMed under a CC BY license. PMID: 34790669

The immunofluorescent staining of ALP, OCN, BMP-2, and AdipoR1 on BMSCs with ADPN 10 μg/ml and AdipoR1 siRNA + ADPN 10 μg/ml, and the corresponding quantification, scale bar = 100 μm.
Index in PubMed under a CC BY license. PMID: 34790669

Expression of AdipoR1 and AdipoR2 in BV2 cells and microglia in mice brain. a RT-PCR analysis of AdipoR1 and AdipoR2 in BV2 cells. b Western blot analysis of AdipoR1 and AdipoR2 expression in BV2 cells. Expression of AdipoR1 and AdipoR2 from cerebral cortex homogenates was used as a positive control. α-Tubulin was used as a loading control. c , d Co-immunocytochemistry staining of microglia (Iba1) and AdipoR1 or AdipoR2 in BV2 cells and microglia in the cortex of WT mice. Scale bar 50 μm
Index in PubMed under a CC BY license. PMID: 31128596

APN suppressed AβO-induced proinflammatory cytokine release in BV2 cells via AdipoR1. a , b Representative Western blot analysis of AdipoR1 and AdipoR2. BV2 cells were transfected with control siRNA, AdipoR1 siRNA, or AdipoR2 siRNA in a concentration-dependent manner (25, 50, 100 nM). c , d ELISA assays of TNFα and IL-1β were conducted after knockdown of AdipoR1. e , f ELISA assays of TNFα and IL-1β were conducted after knockdown of AdipoR2. Data were presented as the mean ± SEM for at least three independent experiments, and each performed in duplicates ( n = 3). Two-way ANOVA with Tukey’s multiple comparison test revealed a difference between groups . *p < 0.05, **p < 0.01, ***p < 0.001; ns, statistically not significant
Index in PubMed under a CC BY license. PMID: 31128596

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Western blot analysis of ADIPOR1 expression in Human heart lysate.