Product Info Summary
SKU: | A10813 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-ADP-dependent glucokinase ADPGK Antibody
SKU/Catalog Number
A10813
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-ADP-dependent glucokinase ADPGK Antibody (Catalog # A10813). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ADP-dependent glucokinase ADPGK Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10813)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Immunogen
Synthesized peptide derived from internal of human ADPGK.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10813 is reactive to Adpgk in Human, Mouse, Rat
Applications
A10813 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
53.902kDa
Background of Adpgk
Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor. GDP and CDP can replace ADP, but with reduced efficiency By similarity.
Wiemann S., Genome Res. 11:422-435(2001).
Ota T., Nat. Genet. 36:40-45(2004).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from Jurkat cells, using ADPGK antibody A10813.
Protein Target Info & Infographic
Gene/Protein Information For Adpgk (Source: Uniprot.org, NCBI)
Gene Name
Adpgk
Full Name
ADP-dependent glucokinase
Weight
53.902kDa
Superfamily
ADP-dependent glucokinase family
Alternative Names
ADP-dependent glucokinase; ADP-GKrbBP-35; ATP-dependent glucokinase; DKFZp434B195; EC 2.7.1.147,2610017G09Rik; RbBP-35 Adpgk|2610017G09Rik, Adp-gk, C77577|ADP-dependent glucokinase|ADP-dependent glucokinase|ATP-dependent glucokinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Adpgk, check out the Adpgk Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Adpgk: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ADP-dependent glucokinase ADPGK Antibody (A10813)
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No publications found for A10813
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Customer Reviews
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Customer Q&As
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Submit your question
6 Customer Q&As for Anti-ADP-dependent glucokinase ADPGK Antibody
Question
I see that the anti-ADPGK antibody A10813 works with WB, what is the protocol used to produce the result images on the product page?
K. Krishna
Verified customer
Asked: 2020-01-01
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-01
Question
We are currently using anti-ADPGK antibody A10813 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
The anti-ADPGK antibody (A10813) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-11
Question
Would anti-ADPGK antibody A10813 work for WB with teratocarcinoma?
Verified Customer
Verified customer
Asked: 2018-11-06
Answer
According to the expression profile of teratocarcinoma, ADPGK is highly expressed in teratocarcinoma. So, it is likely that anti-ADPGK antibody A10813 will work for WB with teratocarcinoma.
Boster Scientific Support
Answered: 2018-11-06
Question
My question regarding product A10813, anti-ADPGK antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Williams
Verified customer
Asked: 2018-02-26
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A10813 anti-ADPGK antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-02-26
Question
Is there a BSA free version of anti-ADPGK antibody A10813 available?
M. Miller
Verified customer
Asked: 2018-01-16
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ADPGK antibody A10813 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-01-16
Question
We need to test anti-ADPGK antibody A10813 on mouse teratocarcinoma for research purposes, then I may be interested in using anti-ADPGK antibody A10813 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
B. Williams
Verified customer
Asked: 2015-04-16
Answer
The products we sell, including anti-ADPGK antibody A10813, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-04-16