Anti-AHNAK1 Purified Monoclonal Antibody

Product Name

Anti-AHNAK1 Purified Monoclonal Antibody

View all AHNAK1 Antibodies

SKU/Catalog Number

M05378

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-AHNAK1 Purified Monoclonal Antibody (Catalog# M05378). Tested in WB, IHC-F, ICC, IP application(s). This antibody reacts with Human, Mouse.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-AHNAK1 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05378)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

EM-09

Isotype

Mouse IgG1

Immunogen

Bacterially expressed fragment of N-terminal domain of human AHNAK1. The mouse monoclonal antibody EM-09 reacts with AHNAK1, a 700 kDa multi-functional adaptor protein expressed mainly in epithelial cell, various types of muscle cells and immune cells.

Cross-reactivity

This antibody does not cross-react with Thy-1.1 alloantigen.

Reactive Species

M05378 is reactive to AHNAK1 in Human, Mouse

Observed Molecular Weight

42 kDa

Calculated molecular weight

629.1 kDa

Background of AHNAK1

AHNAK1 (Desmoyokin) is a large (700 kDa) scaffold protein that translocates to the plasma membrane after an increas of extracellular calcium level or upon proteinkinase C activation and regulates extracellular calcium influx mediated by L-type Ca2+ channels. AHNAK1 has been implicated in diverse signal transduction proceses affecting cell differentiation and proliferation. In response to calcium-dependent intercellular contacts AHNAK1 forms multimeric complexes in the plasma membrane, connected with actin and annexin 2/S100A10 assemblies and is thus involved in organization of the plasma membrane architecture. In epithelial cells, AHNAK1 is localized in cytoplasm or is membrane-associated, but in cells of nonepithelial origin AHNAK1 is predominantly nuclear; it has a weak DNA-binding activity and associates with the DNA-ligase IV-XRCC4 complex.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M05378 is guaranteed for IP, IHC-F, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunocytochemistry: Positive control: HeLa human cervix carcinoma cell line, permeabilization is required.
Western blotting: Positive control: HeLa human cervix carcinoma cell line.

Validation Images & Assay Conditions

See full target information AHNAK1

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