|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-MIF Antibody|
|Description||Rabbit IgG polyclonal antibody for Macrophage migration inhibitory factor(MIF) detection. Tested with WB,IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-MIF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1052)|
|Replacement Item||This antibody may replace the following items: sc-16966|sc-16965|sc-48241|sc-20121|sc-271631|sc-47159|sc-53915|sc-130329 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human MIF(98-115aa NYYDMNAANVGWNNSTFA), different from the related rat and mouse sequences by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Macrophage migration inhibitory factor(MIF)|
|Molecular Weight||12476 MW|
|Protein Function||Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti- inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity. .|
|Sequence Similarities||Belongs to the MIF family.|
|Subcellular Localization||Secreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non- classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.|
|Alternative Names||Macrophage migration inhibitory factor;MIF;220.127.116.11;Glycosylation-inhibiting factor;GIF;L-dopachrome isomerase;L-dopachrome tautomerase;18.104.22.168;Phenylpyruvate tautomerase;MIF;GLIF, MMIF;|
|Research Areas|||immunology|innate immunity|macrophage / inflamm.||
Background for Macrophage migration inhibitory factor(MIF)
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-MIF Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat|
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-MIF Antibody Images
Click the images to enlarge.
Lane 1: HELA Cell Lysate
Lane 2: COLO320 Cell Lysate
Lane 3: MM231 Cell Lysate
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,