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- Table of Contents
AIFM1 antibody (PB9366) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 4 Publications
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Flow Cytometry analysis of Hela cells using anti-AIF antibody (PB9366).
Overlay histogram showing Hela cells stained with PB9366 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AIF Antibody (PB9366,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Overlay histogram showing Hela cells stained with PB9366 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AIF Antibody (PB9366,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Anti-AIF/AIFM1 Antibody Picoband®
AIFM1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
AIFM1 antibody was positively detected by WB in:
human MCF-7 whole cell, human K562 whole cell, human A549 whole cell, human HepG2 whole cell, human Hela whole cell, human Jurkat whole cell, human 293T whole cell, human U251 whole cell, rat stomach tissue, rat kidney tissue, rat NRK whole cell, mouse stomach tissue, mouse kidney tissue, mouse pancreas tissue, mouse NIH/3T3 whole cell,
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
AIFM1 antibody was positively detected by IHC in:
Rat Cardiac Muscle tissue, Human Intestinal Cancer tissue, Mouse Cardiac Muscle tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
AIFM1 antibody was positively detected by ICC/IF in:
NIH3T3 cell, MCF-7 cell
AIFM1 antibody was positively detected by ICC in:
SMMC-7721 cell
AIFM1 antibody was positively detected by FC in:
Hela cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Immunocytochemistry
0.5-1μg/ml
Immunocytochemistry/Immunofluorescence
2μg/ml
Flow Cytometry (Fixed)
1-3μg/1x106 cells
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Zebrafish
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-AIF/AIFM1 Antibody Picoband®
View all AIFM1 Antibodies
View all AIFM1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human AIF, identical to the related mouse and rat sequences.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
67 kDa
Calculated Molecular Weight
66.9 kDa
Background of AIFM1
Apoptosis-inducing factor 1, mitochondrial, also known as AIF or PDCD8 is a protein that in humans is encoded by the AIFM1 gene. AIFM1 gene is mapped to Xq26.1 based on an alignment of the AIFM1 sequence with the genomic sequence. This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. A related pseudogene has been identified on chromosome 10.
Cite This Product
Anti-AIF/AIFM1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9366)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
100%
High Homology
Rat
100%
High Homology
Rabbit
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Dog
100%
High Homology
Monkey
100%
High Homology
Zebrafish
94%
High Homology
Drosophila
56%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for AIFM1 antibody
Immunohistochemistry (IHC) protocol for AIFM1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for AIFM1 antibody
Immunocytochemistry (ICC) protocol for AIFM1 antibody
Flow Cytometry (FC) protocol for AIFM1 antibody
Publications
Application:
Species:
Astaxanthin Protects against Alcoholic Liver Injury via Regulating Mitochondrial Redox Balance and Calcium Homeostasis
Published: 2023-12-01
Journal: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Application: IF
Impact Factor: 6.10
Species: Rat
PMID: 38038704
RETRACTED: Icariin induces apoptosis in acute promyelocytic leukemia by targeting PIM1
Published: 2017-06-15
Journal: Pharmacological Reports
Application: WB
Impact Factor: 2.59
Species: Human
PMID: 29128809
Protective Effects of Nigranoic Acid on Cerebral Ischemia–Reperfusion Injury and its Mechanism Involving Apoptotic Signaling Pathway
Published: 2014-08-29
Journal: CELL BIOCHEMISTRY AND BIOPHYSICS
Application: WB
Impact Factor: 2.38
Species: Rat
PMID: 25168103
Protection by bicyclol derivatives against acetaminophen-induced acute liver failure in mice and its active mechanism
Published: 2008-09-05
Journal: LIVER INTERNATIONAL
Application: WB
Impact Factor: 2.56
Species: Mouse
PMID: 18435715
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Product Promise
We promise Anti-AIF/AIFM1 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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PB9366
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 12, 2026