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- Table of Contents
TNFSF10 antibody (A00466-1) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 6 Publications
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IHC analysis of TRAIL using anti-TRAIL antibody (A00466-1).
TRAIL was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TRAIL Antibody (A00466-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
TRAIL was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TRAIL Antibody (A00466-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Anti-TRAIL/TNFSF10 Antibody Picoband®
TNFSF10 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IHC, WB |
Tested Applications
TNFSF10 antibody was positively detected by WB in:
human HL-60 whole cell, human THP-1 whole cell,
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
TNFSF10 antibody was positively detected by IHC in:
human tonsil tissue, mouse spleen tissue, rat liver tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Mouse, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Mouse
Tested
Recommend
Rat
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-TRAIL/TNFSF10 Antibody Picoband®
View all TNFSF10 Antibodies
View all TNFSF10 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human TRAIL recombinant protein (Position: N40-A272).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
35 kDa
Calculated Molecular Weight
32.5 kDa
Background of TNFSF10
TNF-related apoptosis-inducing ligand (TRAIL), is a protein functioning as a ligand that induces the process of cell death called apoptosis. TRAIL has also been designated CD253 (cluster of differentiation 253). It is a member of Tumor Necrosis Factor Ligand Superfamily. In humans, the gene that encodes for TRAIL is located at chromosome 3q26. TRAIL has got 281 amino acid proteins. TRAIL mRNA is expressed at significant levels in most normal tissues. TRAIL binds to the death receptors DR4 (TRAIL-RI) and DR5 (TRAIL-RII) and the receptors DcR1 and DcR2, leading to activation of specific kinases and transcription of genes.
Cite This Product
Anti-TRAIL/TNFSF10 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00466-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
97%
High Homology
Dog
80%
Moderate Homology
Rabbit
79%
Moderate Homology
Sheep
74%
Moderate Homology
Cow
73%
Moderate Homology
Rat
70%
Moderate Homology
Mouse
67%
Low homology
Zebrafish
44%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for TNFSF10 antibody
Immunohistochemistry (IHC) protocol for TNFSF10 antibody
Publications
Application:
Species:
PARP1 contributes to intimal hyperplasia by regulating METTL3-mediated m6A methylation of TRAIL
Published: 2025-10-16
Journal: CELL BIOLOGY AND TOXICOLOGY
Application: WB
Impact Factor: 5.90
Species: Mouse
PMID: 41099892
Snoopligase-catalyzed molecular glue enables efficient generation of hyperoligomerized TRAIL variant with enhanced antitumor effect
Published: 2023-08-29
Journal: JOURNAL OF CONTROLLED RELEASE
Application: ELISA
Impact Factor: 10.80
Species: Mouse
PMID: 37516318
Tumor necrosis factor-related apoptosis inducing ligand overexpression and Taxol treatment suppresses the growth of cervical cancer cells in vitro and in vivo
Published: 2018-02-16
Journal: Oncology Letters
Application: WB
Impact Factor: 1.66
Species: Human
PMID: 29556305
AAV-iRFP labelling of human mesenchymal stem cells for near-infrared fluorescence imaging
Published: 2017-04-28
Journal: BIOSCIENCE REPORTS
Application: WB
Impact Factor: 2.91
Species: Human
PMID: 28377479
Multifunctional cationic polymer decorated and drug intercalated layered silicate (NLS) for early gastric cancer prevention
Published: 2014-01-16
Journal: BIOMATERIALS
Application: WB
Impact Factor: 8.31
Species: Human
PMID: 24439413
Adenoviral mediated transduction of adenoid cystic carcinoma by human TRAIL gene driven with hTERT tumor specific promoter induces apoptosis
Published: 2009-05-01
Journal: CANCER BIOLOGY & THERAPY
Application: IHC
Impact Factor: 2.76
Species: Human
PMID: 19587531
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Product Promise
We promise Anti-TRAIL/TNFSF10 Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00466-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026