Anti-Ajuba Antibody

Ajuba antibody

Boster Bio Anti-Ajuba Antibody (Catalog # A03320). Tested in ELISA, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A03320
Size: 100ug
Reactive Species: Human
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-Ajuba Antibody

View all Ajuba Antibodies

SKU/Catalog Number

A03320

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Ajuba Antibody (Catalog # A03320). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Ajuba Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03320)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 224-239 of Human Ajuba.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03320 is reactive to Ajuba in Human

Applications

A03320 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

57.92kDa

Background of Ajuba

Human AJUBA (also called JUB protein and ajuba homolog isoform 1) is a LIM domain protein suggested to bind and regulate the activity of Aurora A. Aurora A, which is involved in cell cycle regulation, is upregulated during mitosis, localizing to the centrosomes and microtubule regions proximal to the centrosomes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:20,000 - 1:80,000
IP: 1:100
WB: 1:500 - 1:2,500

Validation Images & Assay Conditions

Gene/Protein Information For Ajuba (Source: Uniprot.org, NCBI)

Gene Name

Ajuba

Full Name

LIM domain-containing protein ajuba

Weight

57.92kDa

Superfamily

zyxin/ajuba family

Alternative Names

Ajuba; JUB; jub, ajuba homolog (Xenopus laevis); MGC15563; protein ajuba Ajuba|Jub|ajuba LIM protein|LIM domain-containing protein ajuba|protein ajuba

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Ajuba, check out the Ajuba Infographic

Ajuba infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Ajuba: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-Ajuba Antibody

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-Ajuba antibody A03320. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-02-26

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-26

Question

I see that the anti-Ajuba antibody A03320 works with IP, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-11-11

Answer

You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-11-11

Question

We are currently using anti-Ajuba antibody A03320 for human tissue, and we are well pleased with the IP results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-22

Answer

The anti-Ajuba antibody (A03320) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-22

Question

Can you help my question with product A03320, anti-Ajuba antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2017-10-06

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03320 anti-Ajuba antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-10-06

Question

Is a blocking peptide available for product anti-Ajuba antibody (A03320)?

M. Williams

Verified customer

Asked: 2016-03-09

Answer

We do provide the blocking peptide for product anti-Ajuba antibody (A03320). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2016-03-09

Size

Total: $599

SKU:A03320

Backordered.

Lead time for this item is typically 4-6 days

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A03320
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$599.00

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