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Product Info Summary
| SKU: | EK1694 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse SCGB3A1 ELISA Kit PicoKine®
View all HIN-1/SCGB3A1 ELISA kits
SKU/Catalog Number
EK1694
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse SCGB3A1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Scgb3a1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml .
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse SCGB3A1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1694)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: F22-G104
Sensitivity
<5 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1694 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1694 is reactive to Scgb3a1 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1694 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of HIN-1/SCGB3A1
Secretoglobin family 3A member 1 is a protein that in humans is encoded by the SCGB3A1 gene. This gene encodes a small secreted protein that may function in the inhibition of cell growth. Expression of the related gene in humans is downregulated via DNA methylation in breast cancers and other tumor types. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1694-CAP | Anti-Mouse Scgb3a1 Pre-coated 96-well strip microplate | 1 |
| EK1694-ST | Mouse Scgb3a1 Standard | 2 vials, 10 ng/tube |
| EK1694-DA | Mouse Scgb3a1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.099 | 0.164 | 0.220 | 0.342 | 0.556 | 0.994 | 1.631 | 2.053 |
Data Example Images
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Mouse SCGB3A1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect HIN-1/SCGB3A1, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 15.6 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse SCGB3A1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 37 | 194 | 396 | 36 | 197 | 415 |
| Standard deviation | 2.73 | 12.8 | 21.78 | 3.06 | 13.59 | 24.9 |
| CV (%) | 7.4% | 6.6% | 5.5% | 8.5% | 6.9% | 6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of HIN-1/SCGB3A1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 37 | 39 | 42 | 37 | 38 | 2.04 | 5.3% |
| Sample 2 | 194 | 168 | 184 | 189 | 183 | 9.75 | 5.3% |
| Sample 3 | 396 | 433 | 480 | 419 | 432 | 30.7 | 7.1% |
Protein Target Info & Infographic
Gene/Protein Information For Scgb3a1 (Source: Uniprot.Org, NCBI)
Gene Name
Scgb3a1
Full Name
Secretoglobin family 3A member 1
Weight
10.591kDa
Superfamily
secretoglobin family
Alternative Names
cytokine high in normal-1; Cytokine HIN-1; High in normal 1; HIN1; HIN-1; HIN1Pneumo secretory protein 2; LU105; PNSP2; PnSP-2; PnSP-2Uteroglobin-related protein 2; SCGB3A1; secretoglobin family 3A member 1; secretoglobin, family 3A, member 1; UGRP2; UGRP2MGC87867 Scgb3a1|HIN, HIN-1, LuLe, LuLeu2, Pnsp2, UGR, UGRP2, Utgrp2|secretoglobin, family 3A, member 1|secretoglobin family 3A member 1|cytokine HIN-1|high in normal 1|pneumo secretory protein 2|uteroglobin related protein 2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Scgb3a1, check out the Scgb3a1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Scgb3a1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse SCGB3A1 ELISA Kit PicoKine® (EK1694)
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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6 Customer Q&As for Mouse SCGB3A1 ELISA Kit PicoKine®
Question
Q: is it okay to use citrate plasma as samples in Mouse HIN-1 Picokine® ELISA Kit (Catalog # EK1694)?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of HIN-1 causing degradation of its protein structure. HIN-1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the HIN-1 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-07-22
Question
Q: if the enzyme conjugated HIN-1 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the HIN-1 antigen?
M. Brown
Verified customer
Asked: 2018-12-22
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-12-22
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-10-06
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-10-06
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-04-05
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-04-05
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-03-12
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-03-12
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2017-02-07
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2017-02-07
