Product Info Summary
SKU: | F00520 |
---|---|
Size: | 50ug |
Reactive Species: | Human, Mouse, Rat |
Host: | Mouse |
Application: | Dot blot, ELISA |
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Product info
Product Name
Anti-AKT3 FITC Monoclonal Antibody
SKU/Catalog Number
F00520
Size
50ug
Form
Lyophilized
Description
Boster Bio Anti-AKT3 FITC Monoclonal Antibody (Catalog # F00520). Tested in Dot blot, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store vial at 4°C prior to restoration. Restore with deionized water (or equivalent). For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration. Expiration date is one (1) year from date of opening.
Cite This Product
Anti-AKT3 FITC Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # F00520)
Host
Mouse
Contents
0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01% (w/v) Sodium Azide
Clonality
Monoclonal
Clone Number
Clone: 25F6.F6.D8 IgG1 kappa
Isotype
IgG1 kappa
Immunogen
Anti-AKT3 Antibody was prepared from tissue culture supernatant by Protein A affinity chromatography using a synthetic peptide corresponding to internal residues of human AKT3 protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
F00520 is reactive to AKT3 in Human, Mouse, Rat
Applications
F00520 is guaranteed for Dot blot, ELISA Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
55.775kDa
Background of AKT3
AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. Anti-AKT3 (MOUSE) PE conjugated Monoclonal Antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience, Signal Transduction research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 50µL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: User optimized
Flow Cytometry: User optimized
Fluorochrome Protein Value: 2.7
IHC: User optimized
IF Microscopy: User optimized
WB: User optimized
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of AKT3 expression in GST Tagged recombinant AKT1 (lane 1), GST Tagged recombinant AKT2 (lane 2) and GST Tagged recombinant AKT3 (lane 3). AKT3 at 78KD was detected using mouse anti-AKT3 unconjugated monoclonal antibody at 1:1000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1001).
Click image to see more details
Dot Blot of mouse anti-AKT3 monoclonal antibody FITC conjugated (Catalog # F00520). Antigen: His-tagged AKT3. Load: Lane 1 -100 ng Lane 2 -33.3 ng Lane 3 -11.1 ng Lane 4 -3.70 ng Lane 5 -1.23 ng. Primary antibody: n/a. Secondary antibody: Mouse anti-AKT3 Monoclonal Antibody Fluorescein Conjugated at 1:1,000 for 60 min at RT. Block: MB-070 for 1 HR at RT.
Click image to see more details
Western blot analysis of AKT3 expression in C2C12 Lysate (lane 1), MEF#1 Lysate (lane 2), MEF#2 Lysate (lane 3), A549 Lysate (lane 4), Calu-1 Lysate (lane 5), PC3 Lysate (lane 6), HepG2 Lysate (lane 7), Jurkat Lysate (lane 8), SKOV3 Lysate (lane 9) and 293T Lsyate (lane 10). AKT3 at 56KD was detected using mouse anti-AKT3 unconjugated monoclonal antibody at 1:1000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1001).
Click image to see more details
Western blot analysis of AKT3 expression in Control (lane 1), Rapa Lysate (lane 2), T50 Lysate (lane 3), T250 Lysate (lane 4), Control (lane 5), Rapa Lysate (lane 6), T50 Lysate (lane 7), T250 Lysate (lane 8) and AKT3 null (lane 9). AKT3 at 56KD was detected using mouse anti-AKT3 unconjugated monoclonal antibody at 1:1000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1001).
Click image to see more details
AKT3 was detected in paraffin-embedded sections of human prostate carcinoma tissue using AKT-3 unconjugated antibody at 10
Click image to see more details
AKT3 was detected in paraffin-embedded sections of mouse embryonic kidney tissue using mouse anti-AKT3 unconjugated monoclonal antibody at 2 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1021).
Protein Target Info & Infographic
Gene/Protein Information For AKT3 (Source: Uniprot.org, NCBI)
Gene Name
AKT3
Full Name
RAC-gamma serine/threonine-protein kinase
Weight
55.775kDa
Superfamily
protein kinase superfamily
Alternative Names
Akt3; Akt-3; EC 2.7.11; EC 2.7.11.1; PKB gamma; PKBGDKFZp434N0250; PRKBG; RAC-gamma; v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) AKT3 MPPH, MPPH2, PKB-GAMMA, PKBG, PRKBG, RAC-PK-gamma, RAC-gamma, STK-2 AKT serine/threonine kinase 3 RAC-gamma serine/threonine-protein kinase|PKB gamma|RAC-gamma serine/threonine protein kinase|v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AKT3, check out the AKT3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AKT3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AKT3 FITC Monoclonal Antibody (F00520)
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No publications found for F00520
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Customer Reviews
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Customer Q&As
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2 Customer Q&As for Anti-AKT3 FITC Monoclonal Antibody
Question
I was wanting to use your anti-AKT3 FITC Monoclonal antibody for IHC for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
As indicated on the product datasheet, F00520 anti-AKT3 FITC Monoclonal antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-05
Question
My question regards to test anti-AKT3 FITC Monoclonal antibody F00520 on human liver for research purposes, then I may be interested in using anti-AKT3 FITC Monoclonal antibody F00520 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
C. Anderson
Verified customer
Asked: 2019-02-15
Answer
The products we sell, including anti-AKT3 FITC Monoclonal antibody F00520, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-02-15