Anti-AKT3 FITC Monoclonal Antibody

AKT3 antibody

Boster Bio Anti-AKT3 FITC Monoclonal Antibody (Catalog # F00520). Tested in Dot blot, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: F00520
Size: 50ug
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: Dot blot, ELISA

Product Name

Anti-AKT3 FITC Monoclonal Antibody

View all AKT3 Antibodies

SKU/Catalog Number

F00520

Size

50ug

Form

Lyophilized

Description

Boster Bio Anti-AKT3 FITC Monoclonal Antibody (Catalog # F00520). Tested in Dot blot, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store vial at 4°C prior to restoration. Restore with deionized water (or equivalent). For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-AKT3 FITC Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # F00520)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01% (w/v) Sodium Azide

Clonality

Monoclonal

Clone Number

Clone: 25F6.F6.D8 IgG1 kappa

Isotype

IgG1 kappa

Immunogen

Anti-AKT3 Antibody was prepared from tissue culture supernatant by Protein A affinity chromatography using a synthetic peptide corresponding to internal residues of human AKT3 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

F00520 is reactive to AKT3 in Human, Mouse, Rat

Applications

F00520 is guaranteed for Dot blot, ELISA Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

55.775kDa

Background of AKT3

AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. Anti-AKT3 (MOUSE) PE conjugated Monoclonal Antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience, Signal Transduction research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 50µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: User optimized
Flow Cytometry: User optimized
Fluorochrome Protein Value: 2.7
IHC: User optimized
IF Microscopy: User optimized
WB: User optimized

Validation Images & Assay Conditions

Gene/Protein Information For AKT3 (Source: Uniprot.org, NCBI)

Gene Name

AKT3

Full Name

RAC-gamma serine/threonine-protein kinase

Weight

55.775kDa

Superfamily

protein kinase superfamily

Alternative Names

Akt3; Akt-3; EC 2.7.11; EC 2.7.11.1; PKB gamma; PKBGDKFZp434N0250; PRKBG; RAC-gamma; v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) AKT3 MPPH, MPPH2, PKB-GAMMA, PKBG, PRKBG, RAC-PK-gamma, RAC-gamma, STK-2 AKT serine/threonine kinase 3 RAC-gamma serine/threonine-protein kinase|PKB gamma|RAC-gamma serine/threonine protein kinase|v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on AKT3, check out the AKT3 Infographic

AKT3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for AKT3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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2 Customer Q&As for Anti-AKT3 FITC Monoclonal Antibody

Question

I was wanting to use your anti-AKT3 FITC Monoclonal antibody for IHC for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?

Verified Customer

Verified customer

Asked: 2020-02-05

Answer

As indicated on the product datasheet, F00520 anti-AKT3 FITC Monoclonal antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-02-05

Question

My question regards to test anti-AKT3 FITC Monoclonal antibody F00520 on human liver for research purposes, then I may be interested in using anti-AKT3 FITC Monoclonal antibody F00520 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

C. Anderson

Verified customer

Asked: 2019-02-15

Answer

The products we sell, including anti-AKT3 FITC Monoclonal antibody F00520, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-02-15

Size

Total: $624

SKU:F00520

Backordered.

Lead time for this item is typically 4-6 days

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F00520
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$624.00

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