Anti-AKT3 FITC Monoclonal Antibody

Product Name

Anti-AKT3 FITC Monoclonal Antibody

View all AKT3 Antibodies

SKU/Catalog Number

F00520

Size

50ug

Form

Lyophilized

Description

Boster Bio Anti-AKT3 FITC Monoclonal Antibody (Catalog # F00520). Tested in Dot blot, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store vial at 4°C prior to restoration. Restore with deionized water (or equivalent). For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration. Expiration date is one (1) year from date of opening. (Ship on wet ice.)

Cite This Product

Anti-AKT3 FITC Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # F00520)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2, antibody formulated with stabilizing protein (10 mg/mL) - Immunoglobulin and Protease free, 0.01% (w/v) Sodium Azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

Clone: 25F6.F6.D8 IgG1 kappa

Isotype

IgG1 kappa

Immunogen

Anti-AKT3 Antibody was prepared from tissue culture supernatant by Protein A affinity chromatography using a synthetic peptide corresponding to internal residues of human AKT3 protein.

Reactive Species

F00520 is reactive to AKT3 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

55.8 kDa

Background of AKT3

AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. Anti-AKT3 (MOUSE) PE conjugated Monoclonal Antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience, Signal Transduction research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

F00520 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: User optimized
Flow Cytometry: User optimized
Fluorochrome Protein Value: 2.7
IHC: User optimized
IF Microscopy: User optimized
WB: User optimized
Anti-AKT3 FITC Antibody has been tested by ELISA and dot blot and is suitable for Flow Cytometry, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT3 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.

Validation Images & Assay Conditions

See full target information AKT3

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