Mouse DcTRAILR1/TNFRSF23 ELISA Kit PicoKine®

DcTRAILR1/TNFRSF23 ELISA kit for Mouse

Mouse DcTRAILR1/TNFRSF23 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tnfrsf23 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK2008
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

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Product Name

Mouse DcTRAILR1/TNFRSF23 ELISA Kit PicoKine®

View all DcTRAILR1/TNFRSF23 ELISA kits

SKU/Catalog Number

EK2008

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse DcTRAILR1/TNFRSF23 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tnfrsf23 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse DcTRAILR1/TNFRSF23 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2008)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: M30-C155

Sensitivity

<10 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2008 is reactive to Tnfrsf23 in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EK2008 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of DcTRAILR1/TNFRSF23

Mouse TNFRSF23, also known as TNFRH1 and DcTRAIL R1, is a GPI-anchored mouse TNF receptor superfamily member that lacks a cytoplasmic death domain and does not induce apoptosis. This gene is mapped to 7 F5; 7 88.28 Cm. This gene encodes a member of the tumor necrosis factor superfamily of proteins. The encoded receptor has been shown to bind to the ligand TRAIL (tumor necrosis factor-related apoptosis-inducing ligand), but to have no signaling capacity. This gene shows elevated expression in mice with diet-induced fatty liver disease. This gene and other family members are present in a gene cluster on chromosome 7. Alternative splicing results in multiple transcript variants.

Kit Components

Catalog Number Description Quantity
EK2008-CAP Anti-Mouse Tnfrsf23 Pre-coated 96-well strip microplate 1
EK2008-ST Mouse Tnfrsf23 Standard 2 vials, 10 ng/tube
EK2008-DA Mouse Tnfrsf23 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0670.1110.1470.2310.3910.7001.3452.226

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect DcTRAILR1/TNFRSF23, Dilution ratio of 1:1, concentration in serum is around 120pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse DcTRAILR1/TNFRSF23 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3111845233124468
Standard deviation1.495.5522.151.686.7031.36
CV (%)4.8%4.7%4.9%5.1%5.4%6.7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of DcTRAILR1/TNFRSF23 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 131323734332.296.9%
Sample 21181211151071155.214.5%
Sample 34524604624604583.840.8%
*number of samples for each test n=16.

Gene/Protein Information For Tnfrsf23 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9ER63

Gene ID

79201

Gene Name

Tnfrsf23

Full Name

Tumor necrosis factor receptor superfamily member 23

Weight

19.595kDa

Alternative Names

DcTRAIL R1; DcTRAILR1; SOB; TNFRH1; TNFRSF23

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Tnfrsf23, check out the Tnfrsf23 Infographic

Tnfrsf23 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Tnfrsf23: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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7 Customer Q&As for Mouse DcTRAILR1/TNFRSF23 ELISA Kit PicoKine®

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2020-05-10

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-05-10

Question

Q: how to analyze ELISA data? I have performed DcTRAILR1 level in plasma.

Verified Customer

Verified customer

Asked: 2020-03-22

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-03-22

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification DcTRAILR1 using the EK2008 Mouse DcTRAILR1 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-09-26

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2019-09-26

Question

Q: What is the optimal O.D. value for DcTRAILR1 ELISA kit? I used your DcTRAILR1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain DcTRAILR1 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2018-09-28

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this DcTRAILR1 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of DcTRAILR1 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2018-09-28

Question

Q: if the enzyme conjugated DcTRAILR1 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the DcTRAILR1 antigen?

Verified Customer

Verified customer

Asked: 2018-05-19

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-05-19

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2017-12-10

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2017-12-10

Question

Q: which procedure should I follow in order to thaw whole blood sample for DcTRAILR1 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2016-04-19

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test DcTRAILR1 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2016-04-19

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