Mouse DcTRAILR2/TNFRSF22 ELISA Kit PicoKine®

DcTRAILR2/TNFRSF22 ELISA kit for Mouse

Mouse DcTRAILR2/TNFRSF22 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tnfrsf22 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK2007
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

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Product Name

Mouse DcTRAILR2/TNFRSF22 ELISA Kit PicoKine®

View all DcTRAILR2/TNFRSF22 ELISA kits

SKU/Catalog Number

EK2007

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse DcTRAILR2/TNFRSF22 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tnfrsf22 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse DcTRAILR2/TNFRSF22 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2007)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: E42-R198

Sensitivity

<10 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2007 is reactive to Tnfrsf22 in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EK2007 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of DcTRAILR2/TNFRSF22

TNFRSF22 interacts via a complex receptor system to preferentially induce apoptosis of tumor cells in vivo and in vitro. This gene is mapped to 7 F5; 7 88.27 cM. The mouse decoy TRAIL R2 (TNFRSF22) gene encodes a 198 amino acid (aa) residues type I transmembrane protein with a putative 39 aa signal peptide, a 136 aa extracellular domain, a 18 aa transmembrane region, and a 5 aa cytoplasmic tail. An alternatively spliced variant encoding a secreted soluble receptor of 180 aa has also been described. The transmembrane and soluble receptors are known as mDcTRAIL R2L and mDcTRAIL R2S, respectively. Both mDcTRAIL R2 isoforms and the GPI-linked mDcTRAIL R1 bind TRAIL specifically but are incapable of triggering signal transduction. Mouse DcTRAIL R1 and mDcTRAIL R2 share 71% sequence identity. The two receptors have similar structural modules in their CRDs, which differ from those found in the CRDs of mTNF RH3. Mouse DcTRAIL R1 preferentially binds mTRAIL while mDcTRAIL R2 binds both human and mTRAIL. mRNA of mouse DcTRAIL R1 and -2 are expressed at low levels in many mouse tissues and at higher levels in several mouse cell lines.

Kit Components

Catalog Number Description Quantity
EK2007-CAP Anti-Mouse Tnfrsf22 Pre-coated 96-well strip microplate 1
EK2007-ST Mouse Tnfrsf22 Standard 2 vials, 10 ng/tube
EK2007-DA Mouse Tnfrsf22 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0160.0360.0820.1610.3370.7341.5682.544

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect DcTRAILR2/TNFRSF22, Dilution ratio of 1:1, concentration in serum is around 40pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse DcTRAILR2/TNFRSF22 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2910246931116475
Standard deviation1.425.4125.332.057.8933.25
CV (%)4.9%5.3%5.4%6.6%6.8%7.0%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of DcTRAILR2/TNFRSF22 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 129343132311.805.8%
Sample 21021051051121063.673.4%
Sample 346950849550749415.723.1%
*number of samples for each test n=16.

Gene/Protein Information For Tnfrsf22 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9ER62

Gene ID

79202

Gene Name

Tnfrsf22

Full Name

Tumor necrosis factor receptor superfamily member 22

Weight

22.375kDa

Alternative Names

DcTRAIL R2; DcTRAILR2; SOBa; TNFRH2; TNFRSF22

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Tnfrsf22, check out the Tnfrsf22 Infographic

Tnfrsf22 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Tnfrsf22: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for EK2007

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6 Customer Q&As for Mouse DcTRAILR2/TNFRSF22 ELISA Kit PicoKine®

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement DcTRAILR2 using the EK2007 Mouse DcTRAILR2 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-04-20

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.

Boster Scientific Support

Answered: 2020-04-20

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2020-01-26

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-01-26

Question

Q: Can DcTRAILR2 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2018-08-19

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2018-08-19

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

P. Miller

Verified customer

Asked: 2018-07-03

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-07-03

Question

Q: What is the optimal O.D. value for DcTRAILR2 ELISA kit? I used your DcTRAILR2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain DcTRAILR2 even though the O.D. values are not very high?

M. Maharaj

Verified customer

Asked: 2016-07-10

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this DcTRAILR2 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of DcTRAILR2 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2016-07-10

Question

Q: we need your suggestion regarding the dilution ratio of serum samples for detection of DcTRAILR2 in Mouse plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.

W. Bhasin

Verified customer

Asked: 2015-10-12

Answer

A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the DcTRAILR2 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2015-10-12

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