Anti-ALDH1A1 Rabbit Monoclonal Antibody

Aldehyde Dehydrogenase 1-A1/ALDH1A1 antibody

Boster Bio Anti-ALDH1A1 Rabbit Monoclonal Antibody catalog # M01392-1. Tested in WB, IHC, ICC/IF, Flow Cytometry, IP applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: M01392-1
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IP, IF, IHC, ICC, WB

Product Name

Anti-ALDH1A1 Rabbit Monoclonal Antibody

View all Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibodies

SKU/Catalog Number

M01392-1

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-ALDH1A1 Rabbit Monoclonal Antibody catalog # M01392-1. Tested in WB, IHC, ICC/IF, Flow Cytometry, IP applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ALDH1A1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01392-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AHA-1

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human ALDH1A1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01392-1 is reactive to ALDH1A1 in Human, Mouse, Rat

Applications

M01392-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

54.862kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For ALDH1A1 (Source: Uniprot.org, NCBI)

Gene Name

ALDH1A1

Full Name

Retinal dehydrogenase 1

Weight

54.862kDa

Superfamily

aldehyde dehydrogenase family

Alternative Names

acetaldehyde dehydrogenase 1; ALDC; aldehyde dehydrogenase 1 family, member A1; aldehyde dehydrogenase 1, soluble; Aldehyde Dehydrogenase 1A1; Aldehyde Dehydrogenase 1-A1; Aldehyde dehydrogenase family 1 member A1; Aldehyde dehydrogenase, cytosolic; aldehyde dehydrogenase, liver cytosolic; ALDH class 1; ALDH1; ALDH11; ALDH1A1; ALDH-E1; ALHDII; EC 1.2.1; EC 1.2.1.36; MGC2318; PUMB1; RALDH 1; RALDH1; retinal dehydrogenase 1; retinaldehyde dehydrogenase 1 ALDH1A1 ALDC, ALDH-E1, ALDH1, ALDH11, HEL-9, HEL-S-53e, HEL12, PUMB1, RALDH1 aldehyde dehydrogenase 1 family member A1 retinal dehydrogenase 1|ALDH class 1|ALHDII|RALDH 1|acetaldehyde dehydrogenase 1|aldehyde dehydrogenase 1, soluble|aldehyde dehydrogenase, liver cytosolic|epididymis luminal protein 12|epididymis luminal protein 9|epididymis secretory sperm binding protein Li 53e|retinaldehyde dehydrogenase 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ALDH1A1, check out the ALDH1A1 Infographic

ALDH1A1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ALDH1A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M01392-1 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Li Y,Chen F,Chen J,Chan S,He Y,Liu W,Zhang G.Disulfiram/Copper Induces Antitumor Activity against Both Nasopharyngeal Cancer Cells and Cancer-Associated Fibroblasts through ROS/MAPK and Ferroptosis Pathways.Cancers (Basel).2020 Jan 6;12(1):138.doi:10.3390
Species: Human,Mouse
M01392-1 usage in article: APP:WB, SAMPLE:NPC CELL LINES AND NP69 CELL, DILUTION:1:100

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16 Customer Q&As for Anti-ALDH1A1 Rabbit Monoclonal Antibody

Question

Is this M01392-1 anti-ALDH1A1 Rabbit Monoclonal antibody reactive to the isotypes of ALDH1A1?

Verified Customer

Verified customer

Asked: 2020-04-14

Answer

The immunogen of M01392-1 anti-ALDH1A1 Rabbit Monoclonal antibody is A synthesized peptide derived from human ALDH1A1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-04-14

Question

Would anti-ALDH1A1 Rabbit Monoclonal antibody M01392-1 work for WB with erythrocyte?

Verified Customer

Verified customer

Asked: 2020-04-09

Answer

According to the expression profile of erythrocyte, ALDH1A1 is highly expressed in erythrocyte. So, it is likely that anti-ALDH1A1 Rabbit Monoclonal antibody M01392-1 will work for WB with erythrocyte.

Boster Scientific Support

Answered: 2020-04-09

Question

I would like using your anti-ALDH1A1 Rabbit Monoclonal antibody for cellular aldehyde metabolic process studies. Has this antibody been tested with western blotting on hepg2 cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-11-01

Answer

We appreciate your inquiry. This M01392-1 anti-ALDH1A1 Rabbit Monoclonal antibody is validated on hepg2 cell lysate. It is guaranteed to work for IP, IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-11-01

Question

I see that the anti-ALDH1A1 Rabbit Monoclonal antibody M01392-1 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-10-17

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-10-17

Question

We bought anti-ALDH1A1 Rabbit Monoclonal antibody for IF on nasal cavity epithelium in the past. I am using mouse, and I plan to use the antibody for IHC next. My question regards examining nasal cavity epithelium as well as liver in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2019-08-26

Answer

I viewed the website and datasheets of our anti-ALDH1A1 Rabbit Monoclonal antibody and it seems that M01392-1 has been validated on mouse in both IF and IHC. Thus M01392-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-08-26

Question

My question regards to test anti-ALDH1A1 Rabbit Monoclonal antibody M01392-1 on rat erythrocyte for research purposes, then I may be interested in using anti-ALDH1A1 Rabbit Monoclonal antibody M01392-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-08-19

Answer

The products we sell, including anti-ALDH1A1 Rabbit Monoclonal antibody M01392-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-08-19

Question

Does M01392-1 anti-ALDH1A1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-08-16

Answer

It shows on the product datasheet, M01392-1 anti-ALDH1A1 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-08-16

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for erythrocyte using anti-ALDH1A1 Rabbit Monoclonal antibody M01392-1. Let me know if you need anything else.

N. Gonzalez

Verified customer

Asked: 2019-07-04

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-07-04

Question

Do you have a BSA free version of anti-ALDH1A1 Rabbit Monoclonal antibody M01392-1 available?

Verified Customer

Verified customer

Asked: 2019-07-02

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-ALDH1A1 Rabbit Monoclonal antibody M01392-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-07-02

Question

We have seen staining in mouse liver. What should we do? Is anti-ALDH1A1 Rabbit Monoclonal antibody supposed to stain liver positively?

Verified Customer

Verified customer

Asked: 2019-05-31

Answer

From what I have seen in literature liver does express ALDH1A1. From what I have seen in Uniprot.org, ALDH1A1 is expressed in nasal cavity epithelium, liver, lens, colon, erythrocyte, among other tissues. Regarding which tissues have ALDH1A1 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Erythrocyte, Pubmed ID: 2776714, 19608861
Lens, Pubmed ID: 19296407
Liver, Pubmed ID: 2987944, 6723659, 8214422, 24275569

Boster Scientific Support

Answered: 2019-05-31

Question

We are currently using anti-ALDH1A1 Rabbit Monoclonal antibody M01392-1 for rat tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-04-26

Answer

The anti-ALDH1A1 Rabbit Monoclonal antibody (M01392-1) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-04-26

Question

My boss were happy with the WB result of your anti-ALDH1A1 Rabbit Monoclonal antibody. However we have observed positive staining in lens cytoplasm using this antibody. Is that expected? Could you tell me where is ALDH1A1 supposed to be expressed?

N. Walker

Verified customer

Asked: 2019-02-05

Answer

From literature, lens does express ALDH1A1. Generally ALDH1A1 expresses in cytoplasm, cytosol. Regarding which tissues have ALDH1A1 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Erythrocyte, Pubmed ID: 2776714, 19608861
Lens, Pubmed ID: 19296407
Liver, Pubmed ID: 2987944, 6723659, 8214422, 24275569

Boster Scientific Support

Answered: 2019-02-05

Question

Please see the WB image, lot number and protocol we used for erythrocyte using anti-ALDH1A1 Rabbit Monoclonal antibody M01392-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-11-09

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-11-09

Question

Is a blocking peptide available for product anti-ALDH1A1 Rabbit Monoclonal antibody (M01392-1)?

P. Baker

Verified customer

Asked: 2017-09-21

Answer

We do provide the blocking peptide for product anti-ALDH1A1 Rabbit Monoclonal antibody (M01392-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-09-21

Question

My question regarding product M01392-1, anti-ALDH1A1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

M. Singh

Verified customer

Asked: 2014-02-27

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01392-1 anti-ALDH1A1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2014-02-27

Question

I was wanting to use your anti-ALDH1A1 Rabbit Monoclonal antibody for WB for rat erythrocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat erythrocyte identification?

L. Parker

Verified customer

Asked: 2014-02-06

Answer

It shows on the product datasheet, M01392-1 anti-ALDH1A1 Rabbit Monoclonal antibody has been tested for IP, IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat erythrocyte in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2014-02-06

Size

Total: $370

SKU:M01392-1

Backordered.

Lead time for this item is typically 3-4 weeks

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M01392-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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