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Product Info Summary
| SKU: | M01392-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ALDH1A1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01392-1
BM4034 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ALDH1A1 Rabbit Monoclonal Antibody catalog # M01392-1. Tested in WB, IHC, ICC/IF, Flow Cytometry, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ALDH1A1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01392-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AHA-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ALDH1A1
Reactive Species
M01392-1 is reactive to ALDH1A1 in Human, Mouse, Rat
Observed Molecular Weight
50 kDa
Calculated molecular weight
54.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01392-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20
Positive Control
WB: human HepG2 whole cell, human A549 whole cell, rat kidney tissue, mouse liver tissue
IHC: human liver cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of ALDH1A1 using anti-ALDH1A1 antibody (M01392-1).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: rat kidney tissue lysates,
Lane 4: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH1A1 antigen affinity purified monoclonal antibody (M01392-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ALDH1A1 at approximately 50 kDa. The expected band size for ALDH1A1 is at 55 kDa.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human liver cancer, using ALDH1A1 Antibody.
Specific Publications For Anti-ALDH1A1 Rabbit Monoclonal Antibody (M01392-1)
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Customer Q&As
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16 Customer Q&As for Anti-ALDH1A1 Rabbit Monoclonal Antibody
Question
Is this M01392-1 anti-ALDH1A1 Rabbit Monoclonal antibody reactive to the isotypes of ALDH1A1?
Verified Customer
Verified customer
Asked: 2020-04-14
Answer
The immunogen of M01392-1 anti-ALDH1A1 Rabbit Monoclonal antibody is A synthesized peptide derived from human ALDH1A1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-14
Question
Would anti-ALDH1A1 Rabbit Monoclonal antibody M01392-1 work for WB with erythrocyte?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
According to the expression profile of erythrocyte, ALDH1A1 is highly expressed in erythrocyte. So, it is likely that anti-ALDH1A1 Rabbit Monoclonal antibody M01392-1 will work for WB with erythrocyte.
Boster Scientific Support
Answered: 2020-04-09
Question
I would like using your anti-ALDH1A1 Rabbit Monoclonal antibody for cellular aldehyde metabolic process studies. Has this antibody been tested with western blotting on hepg2 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
We appreciate your inquiry. This M01392-1 anti-ALDH1A1 Rabbit Monoclonal antibody is validated on hepg2 cell lysate. It is guaranteed to work for IP, IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-11-01
Question
I see that the anti-ALDH1A1 Rabbit Monoclonal antibody M01392-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-10-17
Question
We bought anti-ALDH1A1 Rabbit Monoclonal antibody for IF on nasal cavity epithelium in the past. I am using mouse, and I plan to use the antibody for IHC next. My question regards examining nasal cavity epithelium as well as liver in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
I viewed the website and datasheets of our anti-ALDH1A1 Rabbit Monoclonal antibody and it seems that M01392-1 has been validated on mouse in both IF and IHC. Thus M01392-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-08-26
Question
My question regards to test anti-ALDH1A1 Rabbit Monoclonal antibody M01392-1 on rat erythrocyte for research purposes, then I may be interested in using anti-ALDH1A1 Rabbit Monoclonal antibody M01392-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
The products we sell, including anti-ALDH1A1 Rabbit Monoclonal antibody M01392-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-19
Question
Does M01392-1 anti-ALDH1A1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
It shows on the product datasheet, M01392-1 anti-ALDH1A1 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-16
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for erythrocyte using anti-ALDH1A1 Rabbit Monoclonal antibody M01392-1. Let me know if you need anything else.
N. Gonzalez
Verified customer
Asked: 2019-07-04
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-04
Question
Do you have a BSA free version of anti-ALDH1A1 Rabbit Monoclonal antibody M01392-1 available?
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-ALDH1A1 Rabbit Monoclonal antibody M01392-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-02
Question
We have seen staining in mouse liver. What should we do? Is anti-ALDH1A1 Rabbit Monoclonal antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
From what I have seen in literature liver does express ALDH1A1. From what I have seen in Uniprot.org, ALDH1A1 is expressed in nasal cavity epithelium, liver, lens, colon, erythrocyte, among other tissues. Regarding which tissues have ALDH1A1 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Erythrocyte, Pubmed ID: 2776714, 19608861
Lens, Pubmed ID: 19296407
Liver, Pubmed ID: 2987944, 6723659, 8214422, 24275569
Boster Scientific Support
Answered: 2019-05-31
Question
We are currently using anti-ALDH1A1 Rabbit Monoclonal antibody M01392-1 for rat tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-04-26
Answer
The anti-ALDH1A1 Rabbit Monoclonal antibody (M01392-1) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-26
Question
My boss were happy with the WB result of your anti-ALDH1A1 Rabbit Monoclonal antibody. However we have observed positive staining in lens cytoplasm using this antibody. Is that expected? Could you tell me where is ALDH1A1 supposed to be expressed?
N. Walker
Verified customer
Asked: 2019-02-05
Answer
From literature, lens does express ALDH1A1. Generally ALDH1A1 expresses in cytoplasm, cytosol. Regarding which tissues have ALDH1A1 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Erythrocyte, Pubmed ID: 2776714, 19608861
Lens, Pubmed ID: 19296407
Liver, Pubmed ID: 2987944, 6723659, 8214422, 24275569
Boster Scientific Support
Answered: 2019-02-05
Question
Please see the WB image, lot number and protocol we used for erythrocyte using anti-ALDH1A1 Rabbit Monoclonal antibody M01392-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-11-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-09
Question
Is a blocking peptide available for product anti-ALDH1A1 Rabbit Monoclonal antibody (M01392-1)?
P. Baker
Verified customer
Asked: 2017-09-21
Answer
We do provide the blocking peptide for product anti-ALDH1A1 Rabbit Monoclonal antibody (M01392-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-09-21
Question
My question regarding product M01392-1, anti-ALDH1A1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Singh
Verified customer
Asked: 2014-02-27
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01392-1 anti-ALDH1A1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-02-27
Question
I was wanting to use your anti-ALDH1A1 Rabbit Monoclonal antibody for WB for rat erythrocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat erythrocyte identification?
L. Parker
Verified customer
Asked: 2014-02-06
Answer
It shows on the product datasheet, M01392-1 anti-ALDH1A1 Rabbit Monoclonal antibody has been tested for IP, IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat erythrocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-02-06
