Product Info Summary
SKU: | PA1004-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Alkaline Phosphatase/ALPL Antibody
SKU/Catalog Number
PA1004-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Alkaline Phosphatase/ALPL Antibody catalog # PA1004-1. Tested in WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Alkaline Phosphatase/ALPL Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1004-1)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of rat Alkaline Phosphatase, different from the related mouse sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PA1004-1 is reactive to ALPL in Mouse, Rat
Applications
PA1004-1 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
57 kDa
Calculated molecular weight
57.305kDa
Background of ALPL
Alkaline phosphatase, tissue-nonspecific isozyme is an enzyme that in humans is encoded by the ALPL gene. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. Liver/bone/kidney alkaline phosphatase, also known as tissue-nonspecific alkaline phosphatase, acts physiologically as a lipid-anchored phosphoethanolamine (PEA) and pyridoxal-5-prime-phosphate (PLP) ectophosphatase Mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to a disorder known as hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Rat, Mouse
Validation Images & Assay Conditions
Click image to see more details
All lanes: Anti ALPL (PA1004-1) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Predicted bind size: 57KD
Observed bind size: 57KD
Protein Target Info & Infographic
Gene/Protein Information For ALPL (Source: Uniprot.org, NCBI)
Gene Name
ALPL
Full Name
Alkaline phosphatase, tissue-nonspecific isozyme
Weight
57.305kDa
Superfamily
alkaline phosphatase family
Alternative Names
Akp2; Alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase, liver/bone/kidney; alkaline phosphatase, tissue-nonspecific isozyme; alkaline phosphomonoesterase; ALPL; APTNAP; AP-TNAP; EC 3.1.3.1; FLJ40094; FLJ93059; glycerophosphatase; HOPS; liver/bone/kidney-type alkaline phosphatase; MGC161443; tissue-nonspecific ALP; TNAP; TNSALP; TNSALPMGC167935 ALPL AP-TNAP, APTNAP, HOPS, TNALP, TNAP, TNSALP alkaline phosphatase, biomineralization associated alkaline phosphatase, tissue-nonspecific isozyme|alkaline phosphatase liver/bone/kidney isozyme|alkaline phosphatase, liver/bone/kidney|liver/bone/kidney-type alkaline phosphatase|tissue non-specific alkaline phosphatase|tissue-nonspecific ALP
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ALPL, check out the ALPL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ALPL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Alkaline Phosphatase/ALPL Antibody (PA1004-1)
Hello CJ!
PA1004-1 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
(+)-Cholesten-3-one induces osteogenic differentiation of bone marrow mesenchymal stem cells by activating vitamin D receptor
Liu B, Deng C, Zhang Y, Zhang J. Neural Regen Res. 2012 Jul 5;7(19):1463-8. Doi: 10.3969/J.Issn.1673-5374.2012.19.003. Wnt3A Expression During The Differentiation Of Adipose-Derived Stem Cells Into Cholinergic Neurons.
Su Y, Wang F, Qi H, Zhao Sg, Li X, Cui H. Mol Vis. 2011 Jan 22;17:247-56. Small Interfering Rna Targeting Of S Phase Kinase-Interacting Protein 2 Inhibits Cell Proliferation Of Pterygium Fibroblasts.
Li Q, Xia Yy, Tang Jc, Wang Ry, Bei Cy, Zeng Y. Artif Cells Blood Substit Immobil Biotechnol. 2011 Jun;39(3):137-42. Doi: 10.3109/10731199.2010.502880. Epub 2010 Jul 23. In Vitro And In Vivo Biocompatibility Investigation Of Diamond-Like Carbon Co...
Zhao Jw, Gao Zl, Mei H, Li Yl, Wang Y. Am J Med Sci. 2011 Jun;341(6):460-8. Doi: 10.1097/Maj.0B013E31820865D5. Differentiation Of Human Mesenchymal Stem Cells: The Potential Mechanism For Estrogen-Induced Preferential Osteoblast Versus Adipocyte D...
Zhang P, Wu X, Hu C, Wang P, Li X. In Vitro Cell Dev Biol Anim. 2012 Jan;48(1):30-6. Doi: 10.1007/S11626-011-9471-Y. Epub 2011 Dec 17. Rho Kinase Inhibitor Y-27632 And Accutase Dramatically Increase Mouse Embryonic Stem Cell Derivation.
Shen Z, Ren Y, Ye D, Guo J, Kang C, Ding H. Eur J Histochem. 2011 Mar 21;55(1):E9. Doi: 10.4081/Ejh.2011.E9. Significance And Relationship Between Dj-1 Gene And Surviving Gene Expression In Laryngeal Carcinoma.
Lu Gh, Song Wt, Wang C, Yan Zh. Chemosphere. 2010 Aug;80(9):982-90. Doi: 10.1016/J.Chemosphere.2010.05.038. Epub 2010 Jun 19. Assessment Of In Vivo Estrogenic Response And The Identification Of Environmental Estrogens In The Yangtze River (Nanjing...
Shen Z, Zhan G, Deng H, Ren Y, Ye D, Xiao B, Guo J. J Laryngol Otol. 2013 Dec;127(12):1194-202. Doi: 10.1017/S0022215113003174. Epub 2013 Dec 3. Microrna-519A Demonstrates Significant Tumour Suppressive Activity In Laryngeal Squamous Cells By Targ...
Liu J, Lu G, Wu D, Yan Z. Ecotoxicol Environ Saf. 2014 Apr;102:12-7. Doi: 10.1016/J.Ecoenv.2014.01.014. Epub 2014 Feb 1. A Multi-Biomarker Assessment Of Single And Combined Effects Of Norfloxacin And Sulfamethoxazole On Male Goldfish (Carassius Au...
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