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- Table of Contents
ALPL antibody (PA1004-2) performs well in WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 19 Publications
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All lanes: Anti ALPL (PA1004-2) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: HT1080 Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 57KD
Observed bind size: 57KD
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: HT1080 Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 57KD
Observed bind size: 57KD
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Anti-Alkaline Phosphatase/ALPL Antibody Picoband®
ALPL antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | WB |
Tested Applications
ALPL antibody was positively detected by WB in:
Human Placenta Tissue, HT1080 Whole Cell, JURKAT Whole Cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Alkaline Phosphatase/ALPL Antibody Picoband®
View all ALPL Antibodies
View all ALPL Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Alkaline Phosphatase, different from the related mouse and rat sequences by three amino acids.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
57 kDa
Calculated Molecular Weight
57.3 kDa
Background of ALPL
Alkaline phosphatase (ALPL) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. ALPL is the alkaline phosphatase of skin fibroblasts,the tissue-nonspecific type, and that it is active toward millimolar concentrations of the putative natural substrates phosphoethanolamine (PEA) and pyridoxal-5-prime-phosphate (PLP). ALPL gene exists in single copy in the haploid genome and is composed of 12 exons distributed over more than 50 kb.Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.
Cite This Product
Anti-Alkaline Phosphatase/ALPL Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1004-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Mouse
80%
Moderate Homology
Rat
80%
Moderate Homology
Rabbit
80%
Moderate Homology
Dog
80%
Moderate Homology
Cow
73%
Moderate Homology
Sheep
73%
Moderate Homology
Zebrafish
60%
Low homology
Drosophila
40%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ALPL antibody
Publications
Application:
(+)-Cholesten-3-one induces osteogenic differentiation of bone marrow mesenchymal stem cells by activating vitamin D receptor
PMID: 29218094
Liu B, Deng C, Zhang Y, Zhang J. Neural Regen Res. 2012 Jul 5;7(19):1463-8. Doi: 10.3969/J.Issn.1673-5374.2012.19.003. Wnt3A Expression During The Differentiation Of Adipose-Derived Stem Cells Into Cholinergic Neurons.
PMID: 25657680
Su Y, Wang F, Qi H, Zhao Sg, Li X, Cui H. Mol Vis. 2011 Jan 22;17:247-56. Small Interfering Rna Targeting Of S Phase Kinase-Interacting Protein 2 Inhibits Cell Proliferation Of Pterygium Fibroblasts.
PMID: 21270971
Li Q, Xia Yy, Tang Jc, Wang Ry, Bei Cy, Zeng Y. Artif Cells Blood Substit Immobil Biotechnol. 2011 Jun;39(3):137-42. Doi: 10.3109/10731199.2010.502880. Epub 2010 Jul 23. In Vitro And In Vivo Biocompatibility Investigation Of Diamond-Like Carbon Co...
PMID: 20653335
Zhao Jw, Gao Zl, Mei H, Li Yl, Wang Y. Am J Med Sci. 2011 Jun;341(6):460-8. Doi: 10.1097/Maj.0B013E31820865D5. Differentiation Of Human Mesenchymal Stem Cells: The Potential Mechanism For Estrogen-Induced Preferential Osteoblast Versus Adipocyte D...
PMID: 21289500
Zhang P, Wu X, Hu C, Wang P, Li X. In Vitro Cell Dev Biol Anim. 2012 Jan;48(1):30-6. Doi: 10.1007/S11626-011-9471-Y. Epub 2011 Dec 17. Rho Kinase Inhibitor Y-27632 And Accutase Dramatically Increase Mouse Embryonic Stem Cell Derivation.
PMID: 22179675
Shen Z, Ren Y, Ye D, Guo J, Kang C, Ding H. Eur J Histochem. 2011 Mar 21;55(1):E9. Doi: 10.4081/Ejh.2011.E9. Significance And Relationship Between Dj-1 Gene And Surviving Gene Expression In Laryngeal Carcinoma.
PMID: 21556124
Lu Gh, Song Wt, Wang C, Yan Zh. Chemosphere. 2010 Aug;80(9):982-90. Doi: 10.1016/J.Chemosphere.2010.05.038. Epub 2010 Jun 19. Assessment Of In Vivo Estrogenic Response And The Identification Of Environmental Estrogens In The Yangtze River (Nanjing...
PMID: 20646735
Shen Z, Zhan G, Deng H, Ren Y, Ye D, Xiao B, Guo J. J Laryngol Otol. 2013 Dec;127(12):1194-202. Doi: 10.1017/S0022215113003174. Epub 2013 Dec 3. Microrna-519A Demonstrates Significant Tumour Suppressive Activity In Laryngeal Squamous Cells By Targ...
PMID: 24300127
Liu J, Lu G, Wu D, Yan Z. Ecotoxicol Environ Saf. 2014 Apr;102:12-7. Doi: 10.1016/J.Ecoenv.2014.01.014. Epub 2014 Feb 1. A Multi-Biomarker Assessment Of Single And Combined Effects Of Norfloxacin And Sulfamethoxazole On Male Goldfish (Carassius Au...
PMID: 24580816
Li M, Yan Z, Han W, Zhang Y. Cell Immunol. 2006 Feb;239(2):136-43. Epub 2006 Jun 30. Mimotope Vaccination For Epitope-Specific Induction Of Anti-Cd20 Antibodies.
PMID: 16814270
Guan W, Wang Y, Hou L, Chen L, Li X, Yue W, Ma Y. Poult Sci. 2010 Feb;89(2):312-7. Doi: 10.3382/Ps.2009-00413. Derivation And Characteristics Of Pluripotent Embryonic Germ Cells In Duck.
PMID: 20075284
Lai G, Yan F, Wu J, Leng C, Ju H. Anal Chem. 2011 Apr 1;83(7):2726-32. Doi: 10.1021/Ac103283P. Epub 2011 Mar 3. Ultrasensitive Multiplexed Immunoassay With Electrochemical Stripping Analysis Of Silver Nanoparticles Catalytically Deposited By Gold ...
PMID: 21370869
Wang W, Huang B, Feng Zg, Chen Xp, Tang Wx, Chen Zw. Z Naturforsch C. 2010 Nov-Dec;65(11-12):726-32. Expression, Purification, And Characterization Of Functional Recombinant Human Daintain/Aif-1 In Escherichia Coli.
PMID: 21319716
Zhang Y, Yang J, Jiang S, Fang C, Xiong L, Cheng H, Xia Y. J Clin Immunol. 2012 Dec;32(6):1270-8. Doi: 10.1007/S10875-012-9724-X. Epub 2012 Jun 24. The Lupus-Derived Anti-Double-Stranded Dna Igg Contributes To Myofibroblast-Like Phenotype In Mesan...
PMID: 22730054
Guo P, Shi Zl, Liu A, Lin T, Bi Fg, Shi Mm, Yan Sg. Int J Mol Sci. 2014 Nov 5;15(11):20117-33. Doi: 10.3390/Ijms151120117. Cartilage Oligomeric Matrix Protein Gene Multilayers Inhibit Osteogenic Differentiation And Promote Chondrogenic Differentia...
PMID: 25380520
Song Wt, Lu Gh, Wang C, Zhang Hz, Xu S, Qin J. Bull Environ Contam Toxicol. 2010 Apr;84(4):406-12. Doi: 10.1007/S00128-010-9944-9. Epub 2010 Mar 6. Study On Environmental Estrogen Pollution In Yangtze River (Nanjing Section) By An In Vivo Bioassay.
PMID: 20213193
Xu F, Dong Y, Huang X, Li M, Qin L, Ren Y, Guo F, Chen A, Huang S. Mol Med Rep. 2014 Oct;10(4):1935-41. Doi: 10.3892/Mmr.2014.2430. Epub 2014 Jul 30. Decreased Osteoclastogenesis, Osteoblastogenesis And Low Bone Mass In A Mouse Model Of Type 2 Dia...
PMID: 25109926
Feng L, Zhu M, Zhang M, Jia X, Cheng X, Ding S, Zhu Q. Int Immunopharmacol. 2013 Feb;15(2):206-16. Doi: 10.1016/J.Intimp.2012.11.015. Epub 2012 Dec 5. Amelioration Of Compound 4,4'-Diphenylmethane-Bis(Methyl)Carbamate On High Mobility Group Box1-M...
PMID: 23219582
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PA1004-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Sep 16, 2026