Click image to see more details
Product Info Summary
Customers Who Bought This Also Bought
Anti-Alkaline Phosphatase/ALPL Antibody
Boster Bio Anti-Alkaline Phosphatase/ALPL Antibody catalog # PA1004-2. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Alkaline Phosphatase/ALPL Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1004-2)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
A synthetic peptide corresponding to a sequence at the N-terminus of human Alkaline Phosphatase (31-45aa DQAQETLKYALELQK), different from the related mouse and rat sequences by three amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
PA1004-2 is reactive to ALPL in Human
PA1004-2 is guaranteed for WB Boster Guarantee
Background of ALPL
Alkaline phosphatase (ALPL) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. ALPL is the alkaline phosphatase of skin fibroblasts,the tissue-nonspecific type, and that it is active toward millimolar concentrations of the putative natural substrates phosphoethanolamine (PEA) and pyridoxal-5-prime-phosphate (PLP). ALPL gene exists in single copy in the haploid genome and is composed of 12 exons distributed over more than 50 kb.Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
Submit A Review
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
All lanes: Anti ALPL (PA1004-2) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: HT1080 Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 57KD
Observed bind size: 57KD
Gene/Protein Information For ALPL (Source: Uniprot.Org, NCBI)
Alkaline phosphatase, tissue-nonspecific isozyme
alkaline phosphatase family
Akp2; Alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase, liver/bone/kidney; alkaline phosphatase, tissue-nonspecific isozyme; alkaline phosphomonoesterase; ALPL; APTNAP; AP-TNAP; EC 22.214.171.124; FLJ40094; FLJ93059; glycerophosphatase; HOPS; liver/bone/kidney-type alkaline phosphatase; MGC161443; tissue-nonspecific ALP; TNAP; TNSALP; TNSALPMGC167935 ALPL AP-TNAP, APTNAP, HOPS, TNALP, TNAP, TNSALP alkaline phosphatase, biomineralization associated alkaline phosphatase, tissue-nonspecific isozyme|alkaline phosphatase liver/bone/kidney isozyme|alkaline phosphatase, liver/bone/kidney|liver/bone/kidney-type alkaline phosphatase|tissue non-specific alkaline phosphatase|tissue-nonspecific ALP*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on ALPL, check out the ALPL Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ALPL: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
PA1004-2 has been cited in 19 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
(+)-Cholesten-3-one induces osteogenic differentiation of bone marrow mesenchymal stem cells by activating vitamin D receptor
Liu B, Deng C, Zhang Y, Zhang J. Neural Regen Res. 2012 Jul 5;7(19):1463-8. Doi: 10.3969/J.Issn.1673-5374.2012.19.003. Wnt3A Expression During The Differentiation Of Adipose-Derived Stem Cells Into Cholinergic Neurons.
Su Y, Wang F, Qi H, Zhao Sg, Li X, Cui H. Mol Vis. 2011 Jan 22;17:247-56. Small Interfering Rna Targeting Of S Phase Kinase-Interacting Protein 2 Inhibits Cell Proliferation Of Pterygium Fibroblasts.
Li Q, Xia Yy, Tang Jc, Wang Ry, Bei Cy, Zeng Y. Artif Cells Blood Substit Immobil Biotechnol. 2011 Jun;39(3):137-42. Doi: 10.3109/10731199.2010.502880. Epub 2010 Jul 23. In Vitro And In Vivo Biocompatibility Investigation Of Diamond-Like Carbon Co...
Zhao Jw, Gao Zl, Mei H, Li Yl, Wang Y. Am J Med Sci. 2011 Jun;341(6):460-8. Doi: 10.1097/Maj.0B013E31820865D5. Differentiation Of Human Mesenchymal Stem Cells: The Potential Mechanism For Estrogen-Induced Preferential Osteoblast Versus Adipocyte D...
Zhang P, Wu X, Hu C, Wang P, Li X. In Vitro Cell Dev Biol Anim. 2012 Jan;48(1):30-6. Doi: 10.1007/S11626-011-9471-Y. Epub 2011 Dec 17. Rho Kinase Inhibitor Y-27632 And Accutase Dramatically Increase Mouse Embryonic Stem Cell Derivation.
Shen Z, Ren Y, Ye D, Guo J, Kang C, Ding H. Eur J Histochem. 2011 Mar 21;55(1):E9. Doi: 10.4081/Ejh.2011.E9. Significance And Relationship Between Dj-1 Gene And Surviving Gene Expression In Laryngeal Carcinoma.
Lu Gh, Song Wt, Wang C, Yan Zh. Chemosphere. 2010 Aug;80(9):982-90. Doi: 10.1016/J.Chemosphere.2010.05.038. Epub 2010 Jun 19. Assessment Of In Vivo Estrogenic Response And The Identification Of Environmental Estrogens In The Yangtze River (Nanjing...
Shen Z, Zhan G, Deng H, Ren Y, Ye D, Xiao B, Guo J. J Laryngol Otol. 2013 Dec;127(12):1194-202. Doi: 10.1017/S0022215113003174. Epub 2013 Dec 3. Microrna-519A Demonstrates Significant Tumour Suppressive Activity In Laryngeal Squamous Cells By Targ...
Liu J, Lu G, Wu D, Yan Z. Ecotoxicol Environ Saf. 2014 Apr;102:12-7. Doi: 10.1016/J.Ecoenv.2014.01.014. Epub 2014 Feb 1. A Multi-Biomarker Assessment Of Single And Combined Effects Of Norfloxacin And Sulfamethoxazole On Male Goldfish (Carassius Au...
Li M, Yan Z, Han W, Zhang Y. Cell Immunol. 2006 Feb;239(2):136-43. Epub 2006 Jun 30. Mimotope Vaccination For Epitope-Specific Induction Of Anti-Cd20 Antibodies.
Guan W, Wang Y, Hou L, Chen L, Li X, Yue W, Ma Y. Poult Sci. 2010 Feb;89(2):312-7. Doi: 10.3382/Ps.2009-00413. Derivation And Characteristics Of Pluripotent Embryonic Germ Cells In Duck.
Lai G, Yan F, Wu J, Leng C, Ju H. Anal Chem. 2011 Apr 1;83(7):2726-32. Doi: 10.1021/Ac103283P. Epub 2011 Mar 3. Ultrasensitive Multiplexed Immunoassay With Electrochemical Stripping Analysis Of Silver Nanoparticles Catalytically Deposited By Gold ...
Wang W, Huang B, Feng Zg, Chen Xp, Tang Wx, Chen Zw. Z Naturforsch C. 2010 Nov-Dec;65(11-12):726-32. Expression, Purification, And Characterization Of Functional Recombinant Human Daintain/Aif-1 In Escherichia Coli.
Zhang Y, Yang J, Jiang S, Fang C, Xiong L, Cheng H, Xia Y. J Clin Immunol. 2012 Dec;32(6):1270-8. Doi: 10.1007/S10875-012-9724-X. Epub 2012 Jun 24. The Lupus-Derived Anti-Double-Stranded Dna Igg Contributes To Myofibroblast-Like Phenotype In Mesan...
Guo P, Shi Zl, Liu A, Lin T, Bi Fg, Shi Mm, Yan Sg. Int J Mol Sci. 2014 Nov 5;15(11):20117-33. Doi: 10.3390/Ijms151120117. Cartilage Oligomeric Matrix Protein Gene Multilayers Inhibit Osteogenic Differentiation And Promote Chondrogenic Differentia...
Song Wt, Lu Gh, Wang C, Zhang Hz, Xu S, Qin J. Bull Environ Contam Toxicol. 2010 Apr;84(4):406-12. Doi: 10.1007/S00128-010-9944-9. Epub 2010 Mar 6. Study On Environmental Estrogen Pollution In Yangtze River (Nanjing Section) By An In Vivo Bioassay.
Xu F, Dong Y, Huang X, Li M, Qin L, Ren Y, Guo F, Chen A, Huang S. Mol Med Rep. 2014 Oct;10(4):1935-41. Doi: 10.3892/Mmr.2014.2430. Epub 2014 Jul 30. Decreased Osteoclastogenesis, Osteoblastogenesis And Low Bone Mass In A Mouse Model Of Type 2 Dia...
Feng L, Zhu M, Zhang M, Jia X, Cheng X, Ding S, Zhu Q. Int Immunopharmacol. 2013 Feb;15(2):206-16. Doi: 10.1016/J.Intimp.2012.11.015. Epub 2012 Dec 5. Amelioration Of Compound 4,4'-Diphenylmethane-Bis(Methyl)Carbamate On High Mobility Group Box1-M...
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Have you used Anti-Alkaline Phosphatase/ALPL Antibody?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review Anti-Alkaline Phosphatase/ALPL Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question