Anti-Alkaline Phosphatase/ALPL Antibody

Boster Bio Anti-Alkaline Phosphatase/ALPL Antibody catalog # PA1004. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 22 publication(s).

Product Info Summary

SKU: PA1004
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Alkaline Phosphatase/ALPL Antibody

See all ALPL products

SKU/Catalog Number

PA1004

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Alkaline Phosphatase/ALPL Antibody catalog # PA1004. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Alkaline Phosphatase/ALPL Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1004)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Alkaline Phosphatase (21-35aa,EKEKDPKYWRDQAQE), different from the related rat and mouse sequences by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

PA1004 is reactive to ALPL in Human, Mouse, Rat

Applications

PA1004 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For ALPL (Source: Uniprot.Org, NCBI)

Uniprot ID

P05186

Gene ID

249

Gene Name

ALPL

Full Name

Alkaline phosphatase, tissue-nonspecific isozyme

Weight

57.305kDa

Superfamily

alkaline phosphatase family

Alternative Names

Akp2; Alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase, liver/bone/kidney; alkaline phosphatase, tissue-nonspecific isozyme; alkaline phosphomonoesterase; ALPL; APTNAP; AP-TNAP; EC 3.1.3.1; FLJ40094; FLJ93059; glycerophosphatase; HOPS; liver/bone/kidney-type alkaline phosphatase; MGC161443; tissue-nonspecific ALP; TNAP; TNSALP; TNSALPMGC167935 ALPL AP-TNAP, APTNAP, HOPS, TNALP, TNAP, TNSALP alkaline phosphatase, biomineralization associated alkaline phosphatase, tissue-nonspecific isozyme|alkaline phosphatase liver/bone/kidney isozyme|alkaline phosphatase, liver/bone/kidney|liver/bone/kidney-type alkaline phosphatase|tissue non-specific alkaline phosphatase|tissue-nonspecific ALP

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ALPL, check out the ALPL Infographic

ALPL infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ALPL: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1004 has been cited in 22 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Hou,C.,Wang,X.,Jiang,W.,Bian,Z.,Zhu,L.,& Li,M.(2021).Peptide 11R‑VIVIT promotes fracture healing in osteoporotic rats.International Journal of Molecular Medicine,48,162.https://doi.org/10.3892/ijmm.2021.4995
Species: Rat
PA1004 usage in article: APP:WB, SAMPLE:BMSCS, DILUTION:1:1000

Zhang Y,Yin C,Cheng Y,Huang X,Liu K,Cheng G,Li Z.Electrospinning Nanofiber-Reinforced Aerogels for the Treatment of Bone Defects.Adv Wound Care (New Rochelle).2020 Aug;9(8):441-452.doi:10.1089/wound.2018.0879.Epub 2019 Jun 5.PMID:32857019;PMCID:PMC7382394
Species: Mouse
PA1004 usage in article: APP:IF, SAMPLE:MC3T3-E1 CELL, DILUTION:1:200

(+)-Cholesten-3-one induces osteogenic differentiation of bone marrow mesenchymal stem cells by activating vitamin D receptor

Zhang Y, Wei QS, Ding WB, Zhang LL, Wang HC, Zhu YJ, He W, Chai YN, Liu YW. PLoS One. 2017 Aug 10;12(8):e0182678. doi: 10.1371/journal.pone.0182678. eCollection 2017. Increased microRNA-93-5p inhibits osteogenic differentiation by targeting bone m...

Liu B, Deng C, Zhang Y, Zhang J. Neural Regen Res. 2012 Jul 5;7(19):1463-8. Doi: 10.3969/J.Issn.1673-5374.2012.19.003. Wnt3A Expression During The Differentiation Of Adipose-Derived Stem Cells Into Cholinergic Neurons.

Su Y, Wang F, Qi H, Zhao Sg, Li X, Cui H. Mol Vis. 2011 Jan 22;17:247-56. Small Interfering Rna Targeting Of S Phase Kinase-Interacting Protein 2 Inhibits Cell Proliferation Of Pterygium Fibroblasts.

Li Q, Xia Yy, Tang Jc, Wang Ry, Bei Cy, Zeng Y. Artif Cells Blood Substit Immobil Biotechnol. 2011 Jun;39(3):137-42. Doi: 10.3109/10731199.2010.502880. Epub 2010 Jul 23. In Vitro And In Vivo Biocompatibility Investigation Of Diamond-Like Carbon Co...

Zhao Jw, Gao Zl, Mei H, Li Yl, Wang Y. Am J Med Sci. 2011 Jun;341(6):460-8. Doi: 10.1097/Maj.0B013E31820865D5. Differentiation Of Human Mesenchymal Stem Cells: The Potential Mechanism For Estrogen-Induced Preferential Osteoblast Versus Adipocyte D...

Zhang P, Wu X, Hu C, Wang P, Li X. In Vitro Cell Dev Biol Anim. 2012 Jan;48(1):30-6. Doi: 10.1007/S11626-011-9471-Y. Epub 2011 Dec 17. Rho Kinase Inhibitor Y-27632 And Accutase Dramatically Increase Mouse Embryonic Stem Cell Derivation.

Shen Z, Ren Y, Ye D, Guo J, Kang C, Ding H. Eur J Histochem. 2011 Mar 21;55(1):E9. Doi: 10.4081/Ejh.2011.E9. Significance And Relationship Between Dj-1 Gene And Surviving Gene Expression In Laryngeal Carcinoma.

Lu Gh, Song Wt, Wang C, Yan Zh. Chemosphere. 2010 Aug;80(9):982-90. Doi: 10.1016/J.Chemosphere.2010.05.038. Epub 2010 Jun 19. Assessment Of In Vivo Estrogenic Response And The Identification Of Environmental Estrogens In The Yangtze River (Nanjing...

Shen Z, Zhan G, Deng H, Ren Y, Ye D, Xiao B, Guo J. J Laryngol Otol. 2013 Dec;127(12):1194-202. Doi: 10.1017/S0022215113003174. Epub 2013 Dec 3. Microrna-519A Demonstrates Significant Tumour Suppressive Activity In Laryngeal Squamous Cells By Targ...

Liu J, Lu G, Wu D, Yan Z. Ecotoxicol Environ Saf. 2014 Apr;102:12-7. Doi: 10.1016/J.Ecoenv.2014.01.014. Epub 2014 Feb 1. A Multi-Biomarker Assessment Of Single And Combined Effects Of Norfloxacin And Sulfamethoxazole On Male Goldfish (Carassius Au...

Li M, Yan Z, Han W, Zhang Y. Cell Immunol. 2006 Feb;239(2):136-43. Epub 2006 Jun 30. Mimotope Vaccination For Epitope-Specific Induction Of Anti-Cd20 Antibodies.

Guan W, Wang Y, Hou L, Chen L, Li X, Yue W, Ma Y. Poult Sci. 2010 Feb;89(2):312-7. Doi: 10.3382/Ps.2009-00413. Derivation And Characteristics Of Pluripotent Embryonic Germ Cells In Duck.

Lai G, Yan F, Wu J, Leng C, Ju H. Anal Chem. 2011 Apr 1;83(7):2726-32. Doi: 10.1021/Ac103283P. Epub 2011 Mar 3. Ultrasensitive Multiplexed Immunoassay With Electrochemical Stripping Analysis Of Silver Nanoparticles Catalytically Deposited By Gold ...

Wang W, Huang B, Feng Zg, Chen Xp, Tang Wx, Chen Zw. Z Naturforsch C. 2010 Nov-Dec;65(11-12):726-32. Expression, Purification, And Characterization Of Functional Recombinant Human Daintain/Aif-1 In Escherichia Coli.

Zhang Y, Yang J, Jiang S, Fang C, Xiong L, Cheng H, Xia Y. J Clin Immunol. 2012 Dec;32(6):1270-8. Doi: 10.1007/S10875-012-9724-X. Epub 2012 Jun 24. The Lupus-Derived Anti-Double-Stranded Dna Igg Contributes To Myofibroblast-Like Phenotype In Mesan...

Guo P, Shi Zl, Liu A, Lin T, Bi Fg, Shi Mm, Yan Sg. Int J Mol Sci. 2014 Nov 5;15(11):20117-33. Doi: 10.3390/Ijms151120117. Cartilage Oligomeric Matrix Protein Gene Multilayers Inhibit Osteogenic Differentiation And Promote Chondrogenic Differentia...

Song Wt, Lu Gh, Wang C, Zhang Hz, Xu S, Qin J. Bull Environ Contam Toxicol. 2010 Apr;84(4):406-12. Doi: 10.1007/S00128-010-9944-9. Epub 2010 Mar 6. Study On Environmental Estrogen Pollution In Yangtze River (Nanjing Section) By An In Vivo Bioassay.

Xu F, Dong Y, Huang X, Li M, Qin L, Ren Y, Guo F, Chen A, Huang S. Mol Med Rep. 2014 Oct;10(4):1935-41. Doi: 10.3892/Mmr.2014.2430. Epub 2014 Jul 30. Decreased Osteoclastogenesis, Osteoblastogenesis And Low Bone Mass In A Mouse Model Of Type 2 Dia...

Feng L, Zhu M, Zhang M, Jia X, Cheng X, Ding S, Zhu Q. Int Immunopharmacol. 2013 Feb;15(2):206-16. Doi: 10.1016/J.Intimp.2012.11.015. Epub 2012 Dec 5. Amelioration Of Compound 4,4'-Diphenylmethane-Bis(Methyl)Carbamate On High Mobility Group Box1-M...

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PA1004
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