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- Table of Contents
ALPL antibody (PA1004) performs well in IF, IHC, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 9 Publications
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IHC analysis of ALPL using anti-ALPL antibody (PA1004).
ALPL was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ALPL Antibody (PA1004) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
ALPL was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ALPL Antibody (PA1004) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Alkaline Phosphatase/ALPL Antibody Picoband®
ALPL antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | IF, IHC, ICC, WB |
Tested Applications
ALPL antibody was positively detected by WB in:
human Hela whole cell, human 293T whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ALPL antibody was positively detected by IHC in:
human parotid acinar cell carcinoma tissue, human breast cancer tissue, human acinic cell carcinoma tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
ALPL antibody was positively detected by IHC in:
U2OS cell
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Alkaline Phosphatase/ALPL Antibody Picoband®
View all ALPL Antibodies
View all ALPL Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Alkaline Phosphatase, different from the related rat and mouse sequences by two amino acids.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
80 kDa
Calculated Molecular Weight
57.3 kDa
Background of ALPL
Alkaline phosphatase (ALPL) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. ALPL is the alkaline phosphatase of skin fibroblasts ,the tissue-nonspecific type, and that it is active toward millimolar concentrations of the putative natural substrates phosphoethanolamine (PEA) and pyridoxal-5-prime-phosphate (PLP). ALPL gene exists in single copy in the haploid genome and is composed of 12 exons distributed over more than 50 kb.Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.
Cite This Product
Anti-Alkaline Phosphatase/ALPL Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1004)
Protocols
Western Blot protocol for ALPL antibody
Immunohistochemistry (IHC) protocol for ALPL antibody
Publications
Application:
Species:
Oral Delivery of Bioencapsulated CTB-Pro-IGF-1 Enhances Bone Regeneration in a Pre-Clinical Rat Model of Jaw Osteoradionecrosis
Published: 2026-07-05
Journal: ORAL DISEASES
Application: IHC
Impact Factor: 3.10
Species: Rat
PMID: 42403027
Network pharmacological study and in vitro studies validation-Molecular dynamics simulation of Cistanche deserticola in promoting periodontitis and bone remodeling
Published: 2024-05-21
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: WB
Impact Factor: 4.80
Species: Mouse
PMID: 38776853
WISP2 downregulation inhibits the osteogenic differentiation of BMSCs in congenital scoliosis by regulating Wnt/β-catenin pathway
Published: 2023-06-09
Journal: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
Application: WB
Impact Factor: 6.20
Species: Human
PMID: 37302424
Peptide 11R‑VIVIT promotes fracture healing in osteoporotic rats
Published: 2021-07-02
Journal: INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
Application: WB
Impact Factor: 4.10
Species: Rat
PMID: 34278442
Less Vertebral Bone Mass after Treatment with Macitentan in Mice: A Pilot Study
Published: 2019-02-19
Journal: Biomed Research International
Application: IHC
Impact Factor: 2.20
Species: Mouse
PMID: 30911541
Bone morphogenetic protein 7 promotes odontogenic differentiation of dental pulp stem cells in vitro
Published: 2018-03-16
Journal: LIFE SCIENCES
Application: ICC
Impact Factor: 3.23
Species: Human
PMID: 29555587
Preparation, physicochemical properties and biocompatibility of PBLG/PLGA/bioglass composite scaffolds
Published: 2016-09-30
Journal: Materials Science & Engineering C-Materials for Biological Applications
Impact Factor: 3.42
PMID: 27987675
A comparison of osteocyte bioactivity in fine particulate bone powder grafts vs larger bone grafts in a rat bone repair model
Published: 2014-05-15
Journal: ACTA HISTOCHEMICA
Application: IHC
Impact Factor: 1.76
Species: Rat
PMID: 24835853
Prevention of Osteoporosis in Mice after Ovariectomy via Allograft of Microencapsulated Ovarian Cells
Published: 2010-01-25
Journal: Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology
Application: IHC
Impact Factor: 1.49
Species: Mouse
PMID: 19957338
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Product Promise
We promise Anti-Alkaline Phosphatase/ALPL Antibody Picoband® performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PA1004
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026