Product Info Summary
SKU: | PA1004 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Alkaline Phosphatase/ALPL Antibody
SKU/Catalog Number
PA1004
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Alkaline Phosphatase/ALPL Antibody catalog # PA1004. Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Alkaline Phosphatase/ALPL Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1004)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Alkaline Phosphatase, different from the related rat and mouse sequences by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PA1004 is reactive to ALPL in Human
Applications
PA1004 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
80 kDa
Calculated molecular weight
57.305kDa
Background of ALPL
Alkaline phosphatase (ALPL) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. ALPL is the alkaline phosphatase of skin fibroblasts ,the tissue-nonspecific type, and that it is active toward millimolar concentrations of the putative natural substrates phosphoethanolamine (PEA) and pyridoxal-5-prime-phosphate (PLP). ALPL gene exists in single copy in the haploid genome and is composed of 12 exons distributed over more than 50 kb.Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ALPL using anti-ALPL antibody (PA1004).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALPL antigen affinity purified polyclonal antibody (Catalog # PA1004) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALPL at approximately 80 kDa. The expected band size for ALPL is at 57 kDa.
Click image to see more details
Figure 2. IHC analysis of ALPL using anti-ALPL antibody (PA1004).
ALPL was detected in a paraffin-embedded section of human parotid acinar cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ALPL Antibody (PA1004) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For ALPL (Source: Uniprot.org, NCBI)
Gene Name
ALPL
Full Name
Alkaline phosphatase, tissue-nonspecific isozyme
Weight
57.305kDa
Superfamily
alkaline phosphatase family
Alternative Names
Akp2; Alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase, liver/bone/kidney; alkaline phosphatase, tissue-nonspecific isozyme; alkaline phosphomonoesterase; ALPL; APTNAP; AP-TNAP; EC 3.1.3.1; FLJ40094; FLJ93059; glycerophosphatase; HOPS; liver/bone/kidney-type alkaline phosphatase; MGC161443; tissue-nonspecific ALP; TNAP; TNSALP; TNSALPMGC167935 ALPL AP-TNAP, APTNAP, HOPS, TNALP, TNAP, TNSALP alkaline phosphatase, biomineralization associated alkaline phosphatase, tissue-nonspecific isozyme|alkaline phosphatase liver/bone/kidney isozyme|alkaline phosphatase, liver/bone/kidney|liver/bone/kidney-type alkaline phosphatase|tissue non-specific alkaline phosphatase|tissue-nonspecific ALP
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ALPL, check out the ALPL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ALPL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Alkaline Phosphatase/ALPL Antibody (PA1004)
Hello CJ!
PA1004 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Less Vertebral Bone Mass after Treatment with Macitentan in Mice: A Pilot Study
Preparation, physicochemical properties and biocompatibility of PBLG/PLGA/bioglass composite scaffolds
Prevention of Osteoporosis in Mice after Ovariectomy via Allograft of Microencapsulated Ovarian Cells
Prevention of Osteoporosis in Mice after Ovariectomy via Allograft of Microencapsulated Ovarian Cells
Prevention of Osteoporosis in Mice after Ovariectomy via Allograft of Microencapsulated Ovarian Cells
Hou,C.,Wang,X.,Jiang,W.,Bian,Z.,Zhu,L.,& Li,M.(2021).Peptide 11R‑VIVIT promotes fracture healing in osteoporotic rats.International Journal of Molecular Medicine,48,162.https://doi.org/10.3892/ijmm.2021.4995
Species: Rat
PA1004 usage in article: APP:WB, SAMPLE:BMSCS, DILUTION:1:1000
Zhang Y,Yin C,Cheng Y,Huang X,Liu K,Cheng G,Li Z.Electrospinning Nanofiber-Reinforced Aerogels for the Treatment of Bone Defects.Adv Wound Care (New Rochelle).2020 Aug;9(8):441-452.doi:10.1089/wound.2018.0879.Epub 2019 Jun 5.PMID:32857019;PMCID:PMC7382394
Species: Mouse
PA1004 usage in article: APP:IF, SAMPLE:MC3T3-E1 CELL, DILUTION:1:200
(+)-Cholesten-3-one induces osteogenic differentiation of bone marrow mesenchymal stem cells by activating vitamin D receptor
Zhang Y, Wei QS, Ding WB, Zhang LL, Wang HC, Zhu YJ, He W, Chai YN, Liu YW. PLoS One. 2017 Aug 10;12(8):e0182678. doi: 10.1371/journal.pone.0182678. eCollection 2017. Increased microRNA-93-5p inhibits osteogenic differentiation by targeting bone m...
Liu B, Deng C, Zhang Y, Zhang J. Neural Regen Res. 2012 Jul 5;7(19):1463-8. Doi: 10.3969/J.Issn.1673-5374.2012.19.003. Wnt3A Expression During The Differentiation Of Adipose-Derived Stem Cells Into Cholinergic Neurons.
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