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- Table of Contents
PRKAA1 antibody (M00994) performs well in Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 12 Publications
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Immunofluorescent analysis of Hela cells, using AMPK alpha 1 Antibody .
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Anti-AMPK alpha 1 PRKAA1 Rabbit Monoclonal Antibody
PRKAA1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IP, IF, IHC, ICC, WB |
Tested Applications
PRKAA1 antibody was positively detected by WB in:
human Hela whole cell, human U-87MG whole cell, human PC-3 whole cell, human A549- WT whole cell, human A549-PRKAA1 KO whole cell, rat PC-12 whole cell, rat RH35 whole cell, mouse RAW2647 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
PRKAA1 antibody was positively detected by IHC in:
Human lung adenocarcinoma tissue, Human tonsil tissue, Human pituitary tumor tissue, human kidney tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
PRKAA1 antibody was positively detected by ICC/IF in:
Hela cell
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
IP
FC
Human
Tested
Tested
Recommend
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Product Information
Product Name
Anti-AMPK alpha 1 PRKAA1 Rabbit Monoclonal Antibody
View all PRKAA1 Antibodies
View all PRKAA1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
CDD-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human AMPK alpha 1
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
64 kDa
Calculated Molecular Weight
64.0 kDa
Cite This Product
Anti-AMPK alpha 1 PRKAA1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00994)
Protocols
Western Blot protocol for PRKAA1 antibody
Immunohistochemistry (IHC) protocol for PRKAA1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for PRKAA1 antibody
Publications
Application:
Species:
Function of AMPK/mTOR Signaling in TGF-β1-Induced Pterygium Fibroblast Proliferation and Transdifferentiation
Published: 2025-02-23
Journal: CURRENT EYE RESEARCH
Application: WB
Impact Factor: 2.00
Species: Human
PMID: 39988428
F-53B disrupts energy metabolism by inhibiting the V-ATPase-AMPK axis in neuronal cells
Published: 2025-01-04
Journal: JOURNAL OF HAZARDOUS MATERIALS
Application: WB
Impact Factor: 11.30
Species: Human,Mouse
PMID: 39793390
Dapagliflozin promotes browning of white adipose tissue through the FGFR1-LKB1-AMPK signaling pathway
Published: 2024-04-21
Journal: MOLECULAR BIOLOGY REPORTS
Application: WB
Impact Factor: 2.60
Species: Mouse
PMID: 38644407
Anti-fatigue effects and mechanism of Syngnathus schlegeli peptides supplementation on exercise-fatigued mice
Published: 2023-10-17
Journal: Journal of Functional Foods
Application: WB
Impact Factor: 5.60
Species: Mouse
Lower dose of metformin combined with artesunate induced autophagy-dependent apoptosis of glioblastoma by activating ROS-AMPK-mTOR axis
Published: 2023-07-01
Journal: EXPERIMENTAL CELL RESEARCH
Application: WB
Impact Factor: 3.70
Species: Human
PMID: 37399981
PRKAA1 induces aberrant mitophagy in a PINK1/Parkin-dependent manner, contributing to fluoride-induced developmental neurotoxicity
Published: 2023-03-14
Journal: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
Application: WB
Impact Factor: 6.80
Species: Rat,Human
PMID: 36924562
Quinacrine attenuates diet-induced obesity by inhibiting adipogenesis via activation of AMPK signaling
Published: 2022-11-30
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: WB
Impact Factor: 5.20
Species: Mouse
PMID: 36460132
Microencapsulated hawthorn berry polyphenols alleviate exercise fatigue in mice by regulating AMPK signaling pathway and balancing intestinal microflora
Published: 2022-09-14
Journal: Journal of Functional Foods
Application: WB
Impact Factor: 5.22
Species: Mouse
The Function behind the Relation between Lipid Metabolism and Vimentin on H9N2 Subtype AIV Replication
Published: 2022-08-18
Journal: Viruses-Basel
Application: WB
Impact Factor: 5.82
Species: Human
PMID: 36016436
The effects of Formoterol in preventing adipogenesis and obesity are mediated by PPARγ/C/EBPα axis and AMPK/PGC-1α pathway
Published: 2022-07-04
Journal: BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Application: WB
Impact Factor: 2.34
Species: Mouse
PMID: 35786714
Acyclovir alleviates insulin resistance via activating PKM1 in diabetic mice
Published: 2022-06-22
Journal: LIFE SCIENCES
Application: WB
Impact Factor: 6.78
Species: Mouse
PMID: 35751919
Transient Receptor Potential Melastatin 8 (TRPM8) Channel Regulates Proliferation and Migration of Breast Cancer Cells by Activating the AMPK-ULK1 Pathway to Enhance Basal Autophagy
Published: 2020-12-04
Journal: Frontiers in Oncology
Impact Factor: 4.85
PMID: 33344232
Review
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Product Promise
We promise Anti-AMPK alpha 1 PRKAA1 Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M00994
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 14, 2026