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Product Info Summary
| SKU: | A00994-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-AMPK alpha 1/PRKAA1 Antibody Picoband®
SKU/Catalog Number
A00994-3
Size
100 μg/vial
Form
Lyophilized
Description
Energy-stress signaling: AMPK (PRKAA1) senses AMP/ATP and phosphorylates metabolic enzymes to restore energy balance. Assay context: rabbit polyclonal primary (WB/ELISA; human/mouse/rat); observed ~64 kDa; typical dilutions WB 1:500–1:2000. Frequently profiled with adipokine Leptin and chemokines like CXCL10 to interrogate metabolic–immune interactions; may be related to vesicle biology via ALIX/PDCD6IP in secretory pathway studies (putative).
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AMPK alpha 1/PRKAA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00994-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human AMPK alpha 1 recombinant protein (Position: F377-R446).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00994-3 is reactive to PRKAA1 in Human, Mouse, Rat
Observed Molecular Weight
64 kDa
Calculated molecular weight
64.0 kDa
Background of PRKAA1
5'-AMP-activated protein kinase catalytic subunit alpha-1 is an enzyme that in humans is encoded by the PRKAA1 gene. The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00994-3 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human SiHa whole cell lysates, human Hela whole cell lysates, human RT4 whole cell lysates, human A549 whole cell lysates, rat brain tissue lysates, rat PC-12 whole cell lysates, mouse brain tissue lysates, mouse NIH/3T3 whole cell lysates
Validation Images & Assay Conditions
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Western blot analysis of AMPK alpha 1 using anti-AMPK alpha 1 antibody (A00994-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SiHa whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMPK alpha 1 antigen affinity purified polyclonal antibody (Catalog # A00994-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMPK alpha 1 at approximately 64 kDa. The expected band size for AMPK alpha 1 is at 64 kDa.
Specific Publications For Anti-AMPK alpha 1/PRKAA1 Antibody Picoband® (A00994-3)
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1 Customer Q&As for Anti-AMPK alpha 1/PRKAA1 Antibody Picoband®
Question
We are currently using anti-AMPK alpha 1/PRKAA1 antibody A00994-3 for rat tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
N. Wu
Verified customer
Asked: 2014-06-27
Answer
The anti-AMPK alpha 1/PRKAA1 antibody (A00994-3) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-06-27
