Anti-AMPK alpha1 PRKAA1 Monoclonal Antibody

Boster Bio Anti-AMPK alpha1 PRKAA1 Monoclonal Antibody catalog # A00994-2. Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: A00994-2
Size: 100μl
Reactive Species: Human, Monkey, Mouse, Rat
Host: Mouse
Application: Flow Cytometry, IF, IHC, WB

Product Name

Anti-AMPK alpha1 PRKAA1 Monoclonal Antibody

See all AMPK alpha 1 products

SKU/Catalog Number

A00994-2

Size

100μl

Form

Liquid

Description

Boster Bio Anti-AMPK alpha1 PRKAA1 Monoclonal Antibody catalog # A00994-2. Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-AMPK alpha1 PRKAA1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00994-2)

Host

Mouse

Contents

Ascitic fluid containing 0.03% sodium azide.

Clone Number

2B7

Reactive Species

A00994-2 is reactive to PRKAA1 in Human, Monkey, Mouse, Rat

Applications

A00994-2 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000
IHC, 1:200-1:1000
IF, 1:200-1:1000
FC, 1:200-1:400

Validation Images & Assay Conditions

Gene/Protein Information For PRKAA1 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q13131

Gene ID

5562

Gene Name

PRKAA1

Full Name

5'-AMP-activated protein kinase catalytic subunit alpha-1

Weight

64.009kDa

Superfamily

protein kinase superfamily

Alternative Names

AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase, catalytic, alpha-1,5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK alpha 1; AMPK alpha 1,5'-AMP-activated protein kinase, catalytic alpha-1 chain; AMPK subunit alpha-1; AMPK; AMPK1; AMPKa1; EC 2.7.11; EC 2.7.11.1; MGC33776; MGC57364; PRKAA1; protein kinase, AMP-activated, alpha 1 catalytic subunit PRKAA1 AMPK, AMPKa1 protein kinase AMP-activated catalytic subunit alpha 1 5-AMP-activated protein kinase catalytic subunit alpha-1|5-AMP-activated protein kinase, catalytic alpha-1 chain|ACACA kinase|AMP -activate kinase alpha 1 subunit|AMP-activated protein kinase, catalytic, alpha-1|AMPK alpha 1|AMPK subunit alpha-1|HMGCR kinase|acetyl-CoA carboxylase kinase|hydroxymethylglutaryl-CoA reductase kinase|protein kinase, AMP-activated, alpha 1 catalytic subunit|tau-protein kinase PRKAA1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PRKAA1, check out the PRKAA1 Infographic

PRKAA1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PRKAA1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A00994-2 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Hou S, Zheng F, Li Y, Gao L, Zhang J. Int J Mol Sci. 2014 Aug 26;15(9):15026-43. Doi: 10.3390/Ijms150915026. The Protective Effect Of Glycyrrhizic Acid On Renal Tubular Epithelial Cell Injury Induced By High Glucose.

Have you used Anti-AMPK alpha1 PRKAA1 Monoclonal Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-AMPK alpha1 PRKAA1 Monoclonal Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

5 Customer Q&As for Anti-AMPK alpha1 PRKAA1 Monoclonal Antibody

Question

Do you have a BSA free version of anti-AMPK alpha1 Monoclonal antibody A00994-2 available?

Verified Customer

Verified customer

Asked: 2020-02-24

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-AMPK alpha1 Monoclonal antibody A00994-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-02-24

Question

Does anti-AMPK alpha1 Monoclonal antibody A00994-2 work for WB with liver?

Verified Customer

Verified customer

Asked: 2020-01-06

Answer

According to the expression profile of liver, PRKAA1 is highly expressed in liver. So, it is likely that anti-AMPK alpha1 Monoclonal antibody A00994-2 will work for WB with liver.

Boster Scientific Support

Answered: 2020-01-06

Question

I have a question about product A00994-2, anti-AMPK alpha1 Monoclonal antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-07-31

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00994-2 anti-AMPK alpha1 Monoclonal antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-07-31

Question

Will A00994-2 anti-AMPK alpha1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

G. Anderson

Verified customer

Asked: 2017-03-06

Answer

You can see on the product datasheet, A00994-2 anti-AMPK alpha1 Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-03-06

Question

We are interested in to test anti-AMPK alpha1 Monoclonal antibody A00994-2 on rat liver for research purposes, then I may be interested in using anti-AMPK alpha1 Monoclonal antibody A00994-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

J. Miller

Verified customer

Asked: 2013-10-02

Answer

The products we sell, including anti-AMPK alpha1 Monoclonal antibody A00994-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2013-10-02

Size

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
A00994-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.