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Product Info Summary
| SKU: | A00994-2 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Mouse |
| Application: | ELISA, Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-AMPK alpha1 PRKAA1 Monoclonal Antibody
SKU/Catalog Number
A00994-2
Size
100μl
Form
Liquid
Description
Boster Bio Anti-AMPK alpha1 PRKAA1 Monoclonal Antibody catalog # A00994-2. Tested in WB, IHC, IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat, Monkey.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AMPK alpha1 PRKAA1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00994-2)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
2B7
Isotype
IgG1
Immunogen
Purified recombinant fragment of human AMPKα1 expressed in E. Coli.
Reactive Species
A00994-2 is reactive to PRKAA1 in Human, Monkey, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
64.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00994-2 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Validation Images & Assay Conditions
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Flow cytometric analysis of PC-2 cells using AMPKα1 Monoclonal Antibody (green) and negative control (purple).
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Western Blot analysis using AMPKα1 Monoclonal Antibody against Jurkat (1), HeLa (2), HepG2 (3), MCF-7 (4), Cos7 (5), NIH/3T3 (6), K562 (7), HEK293 (8), and PC-12 (9) cell lysate.
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Immunohistochemistry analysis of paraffin-embedded ovarian cancer (left) and brain tissues (right) with DAB staining using AMPKα1 Monoclonal Antibody.
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Immunofluorescence analysis of NTERA-2 cells using AMPKα1 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Specific Publications For Anti-AMPK alpha1 PRKAA1 Monoclonal Antibody (A00994-2)
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Customer Q&As
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5 Customer Q&As for Anti-AMPK alpha1 PRKAA1 Monoclonal Antibody
Question
Do you have a BSA free version of anti-AMPK alpha1 Monoclonal antibody A00994-2 available?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-AMPK alpha1 Monoclonal antibody A00994-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-24
Question
Does anti-AMPK alpha1 Monoclonal antibody A00994-2 work for WB with liver?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
According to the expression profile of liver, PRKAA1 is highly expressed in liver. So, it is likely that anti-AMPK alpha1 Monoclonal antibody A00994-2 will work for WB with liver.
Boster Scientific Support
Answered: 2020-01-06
Question
I have a question about product A00994-2, anti-AMPK alpha1 Monoclonal antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00994-2 anti-AMPK alpha1 Monoclonal antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-31
Question
Will A00994-2 anti-AMPK alpha1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
G. Anderson
Verified customer
Asked: 2017-03-06
Answer
You can see on the product datasheet, A00994-2 anti-AMPK alpha1 Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-03-06
Question
We are interested in to test anti-AMPK alpha1 Monoclonal antibody A00994-2 on rat liver for research purposes, then I may be interested in using anti-AMPK alpha1 Monoclonal antibody A00994-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Miller
Verified customer
Asked: 2013-10-02
Answer
The products we sell, including anti-AMPK alpha1 Monoclonal antibody A00994-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-10-02
