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- Table of Contents
PRKAA1 antibody (A00994-6) performs well in ELISA, Flow Cytometry, WB and shows reactivity with Human, Monkey, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 13 Publications
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Flow Cytometry analysis of SiHa cells using anti-AMPK alpha 1/PRKAA1 antibody (A00994-6).
Overlay histogram showing SiHa cells stained with A00994-6 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AMPK alpha 1/PRKAA1 Antibody (A00994-6, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing SiHa cells stained with A00994-6 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AMPK alpha 1/PRKAA1 Antibody (A00994-6, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-AMPK alpha 1/PRKAA1 Antibody Picoband®
PRKAA1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Monkey, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, WB |
Tested Applications
PRKAA1 antibody was positively detected by WB in:
human Jurkat whole cell, human Hela whole cell, human MCF-7 whole cell, monkey Cos-7 whole cell, human K572 whole cell, human HEK293 whole cell, rat brain tissue, rat heart tissue, mouse brain tissue, mouse heart tissue, mouse intestines tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
PRKAA1 antibody was positively detected by FC in:
SiHa cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.25μg/ml
Human, Mouse, Rat, Monkey
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
ELISA
Human
Tested
Recommend
Monkey
Tested
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Dog
Predicted
Predicted
Rabbit
Predicted
Predicted
Bovine
Predicted
Predicted
Sheep
Predicted
Predicted
Product Information
Product Name
Anti-AMPK alpha 1/PRKAA1 Antibody Picoband®
View all PRKAA1 Antibodies
View all PRKAA1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human AMPK alpha 1/PRKAA1 recombinant protein (Position: D359-P539).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
64-66 kDa
Calculated Molecular Weight
64.0 kDa
Background of PRKAA1
5'-AMP-activated protein kinase catalytic subunit alpha-1 is an enzyme that in humans is encoded by the PRKAA1 gene. The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.
Cite This Product
Anti-AMPK alpha 1/PRKAA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00994-6)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
99%
High Homology
Dog
99%
High Homology
Rabbit
98%
High Homology
Cow
98%
High Homology
Sheep
98%
High Homology
Rat
97%
High Homology
Mouse
97%
High Homology
Zebrafish
77%
Moderate Homology
Drosophila
43%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for PRKAA1 antibody
Flow Cytometry (FC) protocol for PRKAA1 antibody
Publications
Application:
Species:
ACE2 ameliorates DOX-induced cardiotoxicity by suppressing excessive autophagy via the AMPK/mTOR signaling pathway
Published: 2026-06-01
Journal: BIOCHEMICAL PHARMACOLOGY
Application: WB
Impact Factor: 6.50
Species: Mouse
PMID: 42229735
Effect of metformin on airway inflammation in obese asthmatic mice and study of the mechanism involved
Published: 2025-12-19
Journal: Scientific Reports
Application: WB
Impact Factor: 3.90
Species: Mouse
PMID: 41419506
Unraveling the role of abnormal AMPK and CRMP-2 phosphorylation in developmental fluoride neurotoxicity: Implications for synaptic damage and neurological disorders
Published: 2025-04-15
Journal: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
Application: WB
Impact Factor: 6.10
Species: Rat
PMID: 40239547
F-53B disrupts energy metabolism by inhibiting the V-ATPase-AMPK axis in neuronal cells
Published: 2025-01-04
Journal: JOURNAL OF HAZARDOUS MATERIALS
Application: WB
Impact Factor: 11.30
Species: Human,Mouse
PMID: 39793390
Brown adipose tissue-derived FGF21 mediates the cardioprotection of dexmedetomidine in myocardial ischemia/reperfusion injury
Published: 2024-08-07
Journal: Scientific Reports
Application: WB
Impact Factor: 3.80
Species: Mouse
PMID: 39112671
PRKAA1 induces aberrant mitophagy in a PINK1/Parkin-dependent manner, contributing to fluoride-induced developmental neurotoxicity
Published: 2023-03-14
Journal: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
Application: WB
Impact Factor: 6.80
Species: Rat,Human
PMID: 36924562
The Function behind the Relation between Lipid Metabolism and Vimentin on H9N2 Subtype AIV Replication
Published: 2022-08-18
Journal: Viruses-Basel
Application: WB
Impact Factor: 5.82
Species: Human
PMID: 36016436
Fructus Zanthoxyli extract improves glycolipid metabolism disorder of type 2 diabetes mellitus via activation of AMPK/PI3K/Akt pathway: Network pharmacology and experimental validation
Published: 2022-07-26
Journal: Journal of Integrative Medicine-JIM
Application: WB,IF
Impact Factor: 3.95
Species: Human
PMID: 35965234
Exogenous spermine attenuates diabetic kidney injury in rats by inhibiting AMPK/mTOR signaling pathway
Published: 2021-01-27
Journal: INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
Application: WB
Impact Factor: 4.10
Species: Rat
PMID: 33537831
Exogenous Hydrogen Sulfide Ameliorates Diabetic Myocardial Fibrosis by Inhibiting Cell Aging Through SIRT6/AMPK Autophagy
Published: 2020-07-31
Journal: Frontiers in Pharmacology
Application: WB
Impact Factor: 4.23
Species: Rat
PMID: 32903815
Silica nanoparticles induce spermatocyte cell autophagy through microRNA-494 targeting AKT in GC-2spd cells
Published: 2019-09-11
Journal: ENVIRONMENTAL POLLUTION
Application: WB
Impact Factor: 5.71
Species: Mouse
PMID: 31541822
Effect of metformin on ossification and inflammation of fibroblasts in ankylosing spondylitis: An in vitro study
Published: 2017-07-11
Journal: JOURNAL OF CELLULAR BIOCHEMISTRY
Application: WB
Impact Factor: 3.09
Species: Human
PMID: 28696014
Inhibitory effect of a genistein derivative on pigmentation of guinea pig skin
Published: 2017-01-23
Journal: RSC Advances
Application: IHC
Impact Factor: 3.11
Species: Pig
PMID: 35544430
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Product Promise
We promise Anti-AMPK alpha 1/PRKAA1 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00994-6
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026