Anti-Ankyrin erythroid/ANK/ANK1 Antibody Picoband™ (monoclonal, 5H2E8)

Ankyrin 1 antibody

Boster Bio Anti-Ankyrin erythroid/ANK/ANK1 Antibody Picoband™ (monoclonal, 5H2E8) catalog # M02716-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: M02716-1
Size: 100 μg/vial
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry, WB

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Product Name

Anti-Ankyrin erythroid/ANK/ANK1 Antibody Picoband™ (monoclonal, 5H2E8)

View all Ankyrin 1 Antibodies

SKU/Catalog Number

M02716-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Ankyrin erythroid/ANK/ANK1 Antibody Picoband™ (monoclonal, 5H2E8) catalog # M02716-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Ankyrin erythroid/ANK/ANK1 Antibody Picoband™ (monoclonal, 5H2E8) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02716-1)

Host

Mouse

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Monoclonal

Clone Number

5H2E8

Isotype

Mouse IgG2a

Immunogen

E.coli-derived human Ankyrin erythroid/ANK/ANK1 recombinant protein (Position: N1300-Q1844).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M02716-1 is reactive to ANK1 in Human

Applications

M02716-1 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

206 kDa

Calculated molecular weight

206.265kDa

Background of Ankyrin 1

Ankyrin 1, erythrocytic, also known as ANK1, is a protein that in humans is encoded by the ANK1 gene. Ankyrins are a family of proteins that link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of all patients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving rise to different isoforms of ankyrin 1 have been described. Truncated muscle-specific isoforms of ankyrin 1 resulting from usage of an alternate promoter have also been identified.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry, 1-3 μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For ANK1 (Source: Uniprot.org, NCBI)

Gene Name

ANK1

Full Name

Ankyrin-1

Weight

206.265kDa

Alternative Names

ANK; ANK-1; ankyrin 1, erythrocytic; ankyrin-1; ankyrin-R; Erythrocyte ankyrin; SPH1; SPH2 ANK1 ANK, SPH1, SPH2 ankyrin 1 ankyrin-1|ANK-1|ankyrin 1, erythrocytic|ankyrin-R|erythrocyte ankyrin

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ANK1, check out the ANK1 Infographic

ANK1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ANK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
M02716-1

100μg

$370
M02716-1-10ug

10μg sample (liquid)

$99
M02716-1-Biotin

100 μg Biotin conjugated

$570
M02716-1-Cy3

100 μg Cy3 conjugated

$570
M02716-1-Dylight488

100 μg Dylight488 conjugated

$570
M02716-1-Dylight550

100 μg Dylight550 conjugated

$570
M02716-1-Dylight594

100 μg Dylight594 conjugated

$570
M02716-1-FITC

100 μg FITC conjugated

$570
M02716-1-HRP

100 μg HRP conjugated

$570
M02716-1-APC

100 μg APC conjugated

$670
M02716-1-PE

100 μg PE conjugated

$670
M02716-1-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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M02716-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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