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Product Info Summary
| SKU: | A03253-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Thrombospondin 2/THBS2 Antibody Picoband®
SKU/Catalog Number
A03253-1
Size
100 μg/vial
Form
Lyophilized
Description
THBS2 (thrombospondin-2) is an extracellular matricellular glycoprotein mediating cell–cell/cell–matrix interactions and described on-page as inhibiting tumor growth/angiogenesis and modulating adhesion/migration; UniProt also notes CD36-ligand anti-angiogenic activity (ECM organization; negative regulation of angiogenesis). Assay context: WB with the A03253-1 antibody, commonly interpreted via comparative banding/isoforms in Western blotting workflows. Often contextualized alongside ECM remodeling markers such as MMP3, Human MMP-9 ELISA, and ECM inhibitors like Human TIMP-3 ELISA; collagen crosslinking/fibrosis context can be compared with LOXL2.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Thrombospondin 2/THBS2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03253-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of mouse Thrombospondin 2, which shares 86.6% amino acid (aa) sequence identity with human Thrombospondin 2.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03253-1 is reactive to Thbs2 in Mouse, Rat
Observed Molecular Weight
160 kDa
Calculated molecular weight
129.9 kDa
Background of Thbs2
Thrombospondin-2 (THBS2) is a protein that in humans is encoded by the THBS2 gene. The protein encoded by this gene belongs to the thrombospondin family. The THBS2 is mapped to 6q27 and it is located on chromosome 17. The gene was transcribed in fibroblasts, smooth muscle cells, and an osteosarcoma cell line. It functions as a protein inhibitor of tumor growth and angiogenesis and modulates the cell surface properties of mesenchymal cells and be involved in cell adhesion and migration.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03253-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Positive Control
WB: rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
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Western blot analysis of Thrombospondin 2 using anti-Thrombospondin 2 antibody (A03253-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thrombospondin 2 antigen affinity purified polyclonal antibody (Catalog # A03253-1) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Thrombospondin 2 at approximately 160KD. The expected band size for Thrombospondin 2 is at 130KD.
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Involvement of thrombospondin-2 (THBS2) and microRNA (miR)-128 in hydrogen peroxide-upregulated matrix metalloproteinase-3 (MMP-3) in prostate cancer cells. Comparison of THBS2 ( a ) and miR-128, -134, and -330 ( b ) in PC3 and DU145 prostate cancer cells with or without 50 μM hydrogen peroxide treatment for 48 h through Western blot analysis ( a ) and a real-time RT-PCR ( b ), respectively. Real-time RT-PCR of THBS2 mRNA expression level and ELISA of MMP-3 levels in conditioned media of PC3 transfected with miRNA mimics or inhibitors ( c ). Data are representative of at least three independent experiments and are shown as the mean ± SD. *Student’s t -test * p ≤ 0.05; ** p ≤ 0.001 compared with the control group.
Index in PubMed under a CC BY license. PMID: 28831065
Specific Publications For Anti-Thrombospondin 2/THBS2 Antibody Picoband® (A03253-1)
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Customer Q&As
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1 Customer Q&As for Anti-Thrombospondin 2/THBS2 Antibody Picoband®
Question
Can A03253-1 bind to exosomal THBS2?
Verified customer
Asked: 2021-01-13
Answer
The Anti-Thrombospondin 2/THBS2 Antibody Picoband™ (A03253-1) hasn't been validated to bind to exosomal THBS2.
Boster Scientific Support
Answered: 2021-01-14
